Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A34 F1 I1 R1
|
18 |
56.1 |
3547650 |
96.9% |
3437672 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
1,779,616 |
G→A |
S74S (TCC→TCT) |
thrS ← |
threonyl‑tRNA synthetase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 1,779,616 | 0 | G | A | 100.0%
| 258.5
/ NA
| 65 | S74S (TCC→TCT) | thrS | threonyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (34/31); total (34/31) |
CAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATC > REL606/1779543‑1779688
|
cAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGa > 1:729932/1‑76 (MQ=255)
aaTAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAagag > 2:22250/1‑76 (MQ=255)
tAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTg > 2:1767328/1‑76 (MQ=255)
tAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTg > 2:774246/1‑76 (MQ=255)
tAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTg > 2:39833/1‑76 (MQ=255)
aaCCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGa > 1:656489/1‑76 (MQ=255)
ccGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACg < 2:333159/76‑1 (MQ=255)
cGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGa > 1:1332424/1‑76 (MQ=255)
cGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGa > 2:621562/1‑76 (MQ=255)
ggCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAAt < 2:1560413/76‑1 (MQ=255)
gCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATg < 2:1613044/76‑1 (MQ=255)
cGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGAt > 1:1764566/1‑76 (MQ=255)
cGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGAt < 1:338931/76‑1 (MQ=255)
cGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGAt < 1:930140/76‑1 (MQ=255)
gATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGAtc > 1:1430973/1‑76 (MQ=255)
tGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTcc > 2:1056545/1‑76 (MQ=255)
gCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCa < 1:106042/76‑1 (MQ=255)
ccATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAg > 1:1252753/1‑76 (MQ=255)
cATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGAGACGAATGATCTCCAGa < 2:423385/76‑1 (MQ=255)
aTTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGAc > 1:1229785/1‑76 (MQ=255)
tttGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACct > 1:273806/1‑76 (MQ=255)
tttGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACct > 1:1657731/1‑76 (MQ=255)
tttGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACct > 1:759428/1‑76 (MQ=255)
ttGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACctt < 2:932169/76‑1 (MQ=255)
tGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACcttc < 2:1385816/76‑1 (MQ=255)
gTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACcttctt < 2:579788/76‑1 (MQ=255)
gTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACcttctt < 1:445908/76‑1 (MQ=255)
tATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCttcttc > 1:1583531/1‑76 (MQ=255)
aTGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCg < 1:1620238/76‑1 (MQ=255)
tGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGt < 1:39318/76‑1 (MQ=255)
cGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCt < 2:1004484/76‑1 (MQ=255)
cGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCt > 2:345126/1‑76 (MQ=255)
cGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCt > 1:351720/1‑76 (MQ=255)
cGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCGTCTTCGTCt < 2:1127104/76‑1 (MQ=255)
gCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCttt < 1:113379/76‑1 (MQ=255)
gCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCttt < 1:942334/76‑1 (MQ=255)
gCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCttt < 2:404641/76‑1 (MQ=255)
gttgttTAATCGCGTGCTCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGt > 1:1344667/1‑76 (MQ=255)
gttgttTAATCGCGTGCTCTAACAGGCGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCggg > 1:1340812/1‑75 (MQ=255)
gttgttTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGt < 2:1631058/76‑1 (MQ=255)
ttgttTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTa < 2:1641511/76‑1 (MQ=255)
gttTAATCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAAt < 2:506758/76‑1 (MQ=255)
aaTCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATc > 2:1020736/1‑76 (MQ=255)
tCGCGTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGa > 2:427444/1‑76 (MQ=255)
cgcgTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGAc < 1:1368462/76‑1 (MQ=255)
cgcgTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGAc > 1:889340/1‑76 (MQ=255)
cgcgTGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGAc < 2:480035/76‑1 (MQ=255)
cgcgTGCCCTAACAGGTGCGCACAAGAGGGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGAc > 2:215622/1‑76 (MQ=255)
tGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGtt > 1:1095391/1‑76 (MQ=255)
tGCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGtt > 1:430258/1‑76 (MQ=255)
gCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTtg > 2:1722868/1‑76 (MQ=255)
gCCCTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTtg > 2:460069/1‑76 (MQ=255)
cccTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTtgt < 1:954166/76‑1 (MQ=255)
ccTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTtgtg > 2:467000/1‑76 (MQ=255)
cTAACAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGc > 1:1246205/1‑76 (MQ=255)
aaCAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGcgt < 1:1238595/76‑1 (MQ=255)
aaCAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGcgt > 2:386495/1‑76 (MQ=255)
aaCAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGcgt > 1:242885/1‑76 (MQ=255)
aCAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGcgtc < 2:1397124/76‑1 (MQ=255)
aCAGGTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGcgtc < 2:1260490/76‑1 (MQ=255)
gTGCGCACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGttt < 2:1083403/76‑1 (MQ=255)
cgcACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCa < 1:547358/76‑1 (MQ=255)
cgcACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCa < 1:981109/76‑1 (MQ=255)
gcACAAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCaa < 1:952358/76‑1 (MQ=255)
acaAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATc > 1:23975/1‑76 (MQ=255)
acaAGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATc > 1:1649873/1‑76 (MQ=255)
|
CAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATGATCTCCAGACCTTCTTCGTCTTTGGCGGTAATGATCGACAGTTGTGCGTCGTTTTCAATC > REL606/1779543‑1779688
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
