Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A34 F1 I1 R1
|
18 |
56.1 |
3547650 |
96.9% |
3437672 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,250,438 |
A→C |
S271A (TCA→GCA) |
infB ← |
translation initiation factor IF‑2 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,250,438 | 0 | A | C | 98.4%
| 255.1
/ ‑3.0
| 62 | S271A (TCA→GCA) | infB | translation initiation factor IF‑2 |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (32/29); total (33/29) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.43e-01 |
GCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGAC > REL606/3250366‑3250510
|
gCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTc < 2:403854/76‑1 (MQ=255)
cGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCa < 2:1680075/76‑1 (MQ=255)
cGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCa < 2:11544/76‑1 (MQ=255)
gaagaaCCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAg < 1:969664/76‑1 (MQ=255)
aagaaCCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGc < 1:34504/76‑1 (MQ=255)
aaCCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCgtg > 2:813396/1‑76 (MQ=255)
aaCCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCgtg > 2:1036332/1‑76 (MQ=255)
aaCCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCgtg > 1:1676521/1‑76 (MQ=255)
aCCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCgtgt > 1:1049269/1‑76 (MQ=255)
ccTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGtgtt < 1:7400/76‑1 (MQ=255)
cTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGtgttt < 2:1490216/76‑1 (MQ=255)
tACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGtgtttgtt < 2:1736279/76‑1 (MQ=255)
tACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGtgtttgtt < 2:1324966/76‑1 (MQ=255)
cGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGc < 2:639886/76‑1 (MQ=255)
gTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGcc < 2:365120/76‑1 (MQ=255)
tttCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCtt > 1:357868/1‑76 (MQ=255)
ttCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCttt > 1:1186147/1‑76 (MQ=255)
ttCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCttt < 2:1203945/76‑1 (MQ=255)
ccGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTttct < 1:717151/76‑1 (MQ=255)
ccGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTttct > 1:846273/1‑76 (MQ=255)
cGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTttctt < 1:1080501/76‑1 (MQ=255)
gCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTttcttc < 2:980136/76‑1 (MQ=255)
tttACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGa > 2:518980/1‑76 (MQ=255)
cttACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGa < 2:346230/75‑1 (MQ=255)
ttACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGAc > 1:216828/1‑76 (MQ=255)
ttACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGAc > 1:1610569/1‑76 (MQ=255)
tACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGAcg < 1:669324/76‑1 (MQ=255)
tACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGAcg < 2:392738/76‑1 (MQ=255)
aCCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGAcgc > 1:1470707/1‑76 (MQ=255)
aCCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGAcgc > 1:827706/1‑76 (MQ=255)
ccGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGAcgcg > 1:1194174/1‑76 (MQ=255)
cGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACgcgc > 1:727856/1‑76 (MQ=255)
cGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACgcgc > 1:25328/1‑76 (MQ=255)
ccACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCgctg > 2:1264741/1‑76 (MQ=255)
ccACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCgctg > 1:1006642/1‑76 (MQ=255)
gTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCttt < 2:1181659/76‑1 (MQ=255)
gTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCttt < 2:1357984/76‑1 (MQ=255)
tACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTc < 2:645890/76‑1 (MQ=255)
tACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTc < 2:1364982/76‑1 (MQ=255)
cgttgctgcGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTc > 2:1403384/4‑76 (MQ=255)
aCTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCNTTCTTCGGACGCGCTGCTTTcg < 2:246472/76‑1 (MQ=255)
ctgctgCGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTcgc < 2:1613717/76‑1 (MQ=255)
ctgcGCGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGtt < 2:622822/76‑1 (MQ=255)
gcgcGTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTAc > 2:698391/1‑76 (MQ=255)
gTGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGAcc > 1:121953/1‑76 (MQ=255)
tGCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGAcca > 1:1043443/1‑76 (MQ=255)
gCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGAccac > 1:803242/1‑76 (MQ=255)
gCTTCTTCACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGAccac > 1:365640/1‑76 (MQ=255)
cttcACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGAccacggcc > 2:992057/1‑76 (MQ=255)
cttcACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGAccacggcc > 1:1311398/1‑76 (MQ=255)
ttcACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGAccacggcca < 1:373077/76‑1 (MQ=255)
tcACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGAccacggccac < 2:900256/76‑1 (MQ=255)
cACGATCAGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTCCGCGTTACGAccacggccacg > 1:958476/1‑76 (MQ=255)
cACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTAAGAccacggccacg > 1:563880/1‑76 (MQ=255)
aGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCt < 1:61435/76‑1 (MQ=255)
aGCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCt > 1:811025/1‑76 (MQ=255)
gCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGgccgccct > 1:992312/1‑74 (MQ=255)
gCTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCtt < 2:1592045/76‑1 (MQ=255)
cTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTc > 1:772137/1‑76 (MQ=255)
cTTTTGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTc > 1:1352302/1‑76 (MQ=255)
tttGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGa > 2:369997/1‑76 (MQ=255)
ttGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGAc > 2:742413/1‑76 (MQ=255)
ttGCTTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCCCCGAc > 1:616602/1‑76 (MQ=255)
|
GCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGAC > REL606/3250366‑3250510
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
