Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A43 F1 I1 R1
|
17 |
61.8 |
3886062 |
97.4% |
3785024 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,339,247 |
A→G |
D29G (GAC→GGC) |
fis → |
DNA‑binding protein Fis |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,339,247 | 0 | A | G | 98.7%
| 325.7
/ ‑3.8
| 79 | D29G (GAC→GGC) | fis | DNA‑binding protein Fis |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (41/37); total (42/37) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.26e-01 |
CGCGTAAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGACCTCTATGAGC > REL606/3339174‑3339318
|
cgcgTAAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCt > 2:1147301/1‑76 (MQ=255)
gcgTAAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTc < 1:1503988/76‑1 (MQ=255)
cgTAAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCg > 1:455339/1‑76 (MQ=255)
aaaTTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGtt > 2:541194/1‑76 (MQ=255)
aaaTTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGtt < 2:192181/76‑1 (MQ=255)
aaaTTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGtt < 2:327918/76‑1 (MQ=255)
aaTTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCANAAACCCCTGCGTGGCTCGGTTa < 2:256746/76‑1 (MQ=255)
aaTTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTa > 1:724547/1‑76 (MQ=255)
tCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAACCCCCTGCGTGGCTCGGTTAAAc < 1:395271/76‑1 (MQ=255)
tCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTaaaa < 1:961893/76‑2 (MQ=255)
cTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACa < 1:441838/76‑1 (MQ=255)
tGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAg > 2:64529/1‑76 (MQ=255)
gACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAgg > 2:958842/1‑76 (MQ=255)
gACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAgg < 2:1241287/76‑1 (MQ=255)
gACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAgg > 1:1644256/1‑76 (MQ=255)
aCGTACTGACTGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGc < 1:826372/76‑1 (MQ=255)
aCGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGc < 1:189541/76‑1 (MQ=255)
aCGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGc > 2:1797036/1‑76 (MQ=255)
cGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCa < 1:1080015/76‑1 (MQ=255)
tACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACt > 1:1283623/1‑76 (MQ=255)
tACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACt > 1:498138/1‑76 (MQ=255)
tACTGACCGTTTCTACCGTTAACTATCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACt > 2:1818141/1‑76 (MQ=255)
aCTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTg > 2:1239595/1‑76 (MQ=255)
tGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTgaa > 2:1865156/1‑76 (MQ=255)
tGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTgaa > 2:908667/1‑76 (MQ=255)
gACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTgaag < 1:1845347/76‑1 (MQ=255)
gACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTgaag > 2:475256/1‑76 (MQ=255)
gACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTgaag < 1:1601029/76‑1 (MQ=255)
aCCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTgaaga < 2:1208998/76‑1 (MQ=255)
ccGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTgaagaa > 1:315783/1‑76 (MQ=255)
tttCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTa < 2:1272200/76‑1 (MQ=255)
tttCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTa > 1:1257493/1‑76 (MQ=255)
tCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTAtt < 2:1241019/76‑1 (MQ=255)
tCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTAtt < 2:1241026/76‑1 (MQ=255)
cTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTAttt > 2:203768/1‑76 (MQ=255)
tACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTAtttt < 1:615468/76‑1 (MQ=255)
tACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTAtttt < 1:1750105/76‑1 (MQ=255)
tACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTAtttt > 1:1603591/1‑76 (MQ=255)
aCCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTg < 2:858877/76‑1 (MQ=255)
ccGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGc < 1:752979/76‑1 (MQ=255)
ccGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGc < 1:795757/76‑1 (MQ=255)
cGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCt < 1:871580/76‑1 (MQ=255)
ttAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCa < 1:684187/76‑1 (MQ=255)
aaCTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAAc > 1:1032747/1‑76 (MQ=255)
aCTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACt < 1:743665/76‑1 (MQ=255)
aCTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACt < 1:357872/76‑1 (MQ=255)
ctctCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTg > 2:988785/1‑76 (MQ=255)
ctctCAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTg > 1:647264/1‑76 (MQ=255)
ctcAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGaa < 2:991004/76‑1 (MQ=255)
ctcAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGaa > 1:1639946/1‑76 (MQ=255)
ctcAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGaa > 1:452267/1‑76 (MQ=255)
ctcAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGaa > 2:1544979/1‑76 (MQ=255)
ctcAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGaa < 1:502653/76‑1 (MQ=255)
tcAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAAt > 1:1623484/1‑76 (MQ=255)
cAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATg > 2:1143737/1‑76 (MQ=255)
cAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATg < 2:1827563/76‑1 (MQ=255)
cAGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATg < 2:796963/76‑1 (MQ=255)
aGGATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATgg > 1:657792/1‑76 (MQ=255)
gATCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTc > 1:565960/1‑76 (MQ=255)
tCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAg < 2:90541/76‑1 (MQ=255)
tCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAg < 2:736366/76‑1 (MQ=255)
tCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAg > 1:204700/1‑76 (MQ=255)
tCAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAg < 1:143039/76‑1 (MQ=255)
cAGGTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAgg < 1:1144639/76‑1 (MQ=255)
gTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGAtg < 1:1916623/76‑1 (MQ=255)
gTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGAtg > 1:176923/1‑76 (MQ=255)
gTAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGAtg > 1:488167/1‑76 (MQ=255)
tAACCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGAtgt > 2:785678/1‑76 (MQ=255)
aaCCCAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGAtgtg > 2:1062360/1‑76 (MQ=255)
cccAAAAACCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGaa < 1:1484931/76‑1 (MQ=255)
aaaaaCCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGa > 1:1664397/1‑76 (MQ=255)
aaaaaCCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGa < 2:897080/76‑1 (MQ=255)
aaaaaCCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGa > 1:508397/1‑76 (MQ=255)
aaaaCCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGAc > 2:1431043/1‑76 (MQ=255)
aaaaCCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGAc > 2:1421237/1‑76 (MQ=255)
aaaCCCCTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGAcc > 1:315922/1‑76 (MQ=255)
ccccTGCGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGACctct > 2:558659/1‑76 (MQ=255)
cGTGGCTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGACCTCTATGAGc > 1:148867/1‑76 (MQ=255)
cGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGACCTCTATGAGc > 2:1678379/1‑76 (MQ=255)
|
CGCGTAAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTCAGGATCAGGTAACCCAAAAACCCCTGCGTGACTCGGTTAAACAGGCACTGAAGAACTATTTTGCTCAACTGAATGGTCAGGATGTGAATGACCTCTATGAGC > REL606/3339174‑3339318
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
