Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A43 F1 I1 R1
|
17 |
61.8 |
3886062 |
97.4% |
3785024 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,162,570 |
T→A |
I572N (ATC→AAC) |
rpoB → |
DNA‑directed RNA polymerase subunit beta |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,162,570 | 0 | T | A | 100.0%
| 277.9
/ NA
| 69 | I572N (ATC→AAC) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (35/34); total (35/34) |
GAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGA > REL606/4162498‑4162642
|
gagaCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACaa > 2:1370599/1‑76 (MQ=255)
gagaCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACaa > 2:98094/1‑76 (MQ=255)
cGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTTAACAActct < 1:1680539/76‑1 (MQ=255)
cGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAActct < 2:1325558/76‑1 (MQ=255)
cGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAActct > 2:459008/1‑76 (MQ=255)
cGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAActct < 2:1648349/76‑1 (MQ=255)
cGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAActct > 2:1797889/1‑76 (MQ=255)
cGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAActca < 2:500955/76‑2 (MQ=255)
gTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAActctc > 1:854044/1‑76 (MQ=255)
acacCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTg > 2:1726071/1‑76 (MQ=255)
aCTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCgtgt < 1:331524/76‑1 (MQ=255)
cTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTa < 2:1466795/76‑1 (MQ=255)
cACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTAca < 1:1704563/76‑2 (MQ=255)
cACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACg > 1:1269628/1‑76 (MQ=255)
cACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACg > 2:574050/1‑76 (MQ=255)
cACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACg > 1:1593438/1‑76 (MQ=255)
cACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACg > 1:1381048/1‑76 (MQ=255)
cACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACg < 2:749754/76‑1 (MQ=255)
cTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGcc > 2:1185474/1‑75 (MQ=255)
ggTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGa > 1:793107/1‑76 (MQ=255)
cgcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTa < 1:1282816/76‑1 (MQ=255)
cgcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTa < 1:1408996/76‑1 (MQ=255)
gcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTaa < 1:992175/76‑1 (MQ=255)
cgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAAc > 1:1774550/1‑76 (MQ=255)
cgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAAc > 2:866104/1‑76 (MQ=255)
gTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACg < 1:743253/76‑1 (MQ=255)
gTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACg < 1:589078/76‑1 (MQ=255)
tATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGa > 1:1017267/1‑76 (MQ=255)
tATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGa > 1:283119/1‑76 (MQ=255)
tGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAAt < 1:53039/76‑1 (MQ=255)
tGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAAt > 2:1107940/1‑76 (MQ=255)
gTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATa < 1:991375/76‑1 (MQ=255)
tCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATAc < 1:440449/76‑1 (MQ=255)
tCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATAc < 1:1224365/76‑1 (MQ=255)
tCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATAc < 1:1914059/76‑1 (MQ=255)
tCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATAc < 1:954076/76‑1 (MQ=255)
ccAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACg > 1:1172368/1‑76 (MQ=255)
aaTCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGc > 1:1415986/1‑76 (MQ=255)
aaTCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGc < 1:116186/76‑1 (MQ=255)
tCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCtt < 1:505195/76‑1 (MQ=255)
tCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCtt < 1:862250/76‑1 (MQ=255)
tCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCtt < 1:305034/76‑1 (MQ=255)
tCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCtt < 2:459407/76‑1 (MQ=255)
cGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTc > 1:549714/1‑76 (MQ=255)
cGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTc > 1:1093762/1‑76 (MQ=255)
aaaCCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGACTACGGCTTCCt > 2:1504406/1‑76 (MQ=255)
aaaCCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCt > 1:1852809/1‑76 (MQ=255)
aaaCCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCt > 2:1504390/1‑76 (MQ=255)
ccccTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTga > 2:636891/1‑76 (MQ=255)
cTGAAGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGAc > 1:105321/1‑76 (MQ=255)
aaGGTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTTAGACTcc > 1:1051633/1‑76 (MQ=255)
ggTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGt < 1:925579/76‑1 (MQ=255)
ggTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGt < 2:340375/76‑1 (MQ=255)
ggTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGt > 2:587729/1‑76 (MQ=255)
gTCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTa < 2:79274/76‑1 (MQ=255)
tCCGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTAt > 2:1100255/1‑76 (MQ=255)
ccGAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATc > 1:340475/1‑76 (MQ=255)
gAACATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGt > 2:1289917/1‑76 (MQ=255)
cATCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTaaa < 1:1379641/76‑1 (MQ=255)
aTCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAg > 2:1683263/1‑76 (MQ=255)
tCGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGt < 1:256962/76‑1 (MQ=255)
cGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTg < 1:1251055/76‑1 (MQ=255)
cGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTg > 1:591143/1‑76 (MQ=255)
cGGTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTg > 1:69079/1‑76 (MQ=255)
ggTCTGAACAACTCTCTGTCCGTGTACGCTCAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGa < 2:330607/76‑1 (MQ=255)
ggTCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGa < 2:35964/76‑1 (MQ=255)
tCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGAcc < 1:597792/76‑1 (MQ=255)
tCTGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGAcc < 1:134018/76‑1 (MQ=255)
tGAACAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGa > 1:458849/1‑76 (MQ=255)
|
GAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGA > REL606/4162498‑4162642
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
