Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A131 F1 I1 R1 13 244.3 15300636 95.6% 14627408 76.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 4,162,570 T→A I572N (ATC→AAC)  rpoB → DNA‑directed RNA polymerase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6064,162,5700TA99.2% 956.2 / ‑0.6 236I572N (ATC→AAC) rpoBDNA‑directed RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base A (123/110);  total (125/111)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.49e-01

Only 100 of 239 total aligned reads displayed.

CGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGA  >  REL606/4162497‑4162642
                                                                         |                                                                          
cGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACa                                                                          >  1:2219465/1‑76 (MQ=255)
 gagaCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACaa                                                                         <  1:55643/76‑1 (MQ=255)
 gagaCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACaa                                                                         <  1:7346874/76‑1 (MQ=255)
    aCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑ctc                                                                    >  1:3001584/1‑76 (MQ=255)
    aCGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑ctc                                                                    <  1:7568937/76‑1 (MQ=255)
     cGTACCCCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑ctct                                                                   <  2:4772372/76‑1 (MQ=255)
      gTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑ctctc                                                                  >  2:5202615/1‑76 (MQ=255)
       tACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑ctctct                                                                 <  2:2910145/76‑1 (MQ=255)
         cacCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGt                                                               >  2:1194196/1‑76 (MQ=255)
           cccGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTcc                                                             >  1:1330218/1‑76 (MQ=255)
           cccGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTcc                                                             >  1:4845265/1‑76 (MQ=255)
           cccGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTcc                                                             >  1:3616058/1‑76 (MQ=255)
              gACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCgtg                                                          >  2:2172426/1‑76 (MQ=255)
                cTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTa                                                        <  2:3094323/76‑1 (MQ=255)
                cTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTa                                                        <  2:4369090/76‑1 (MQ=255)
                cTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTa                                                        >  1:5793868/1‑76 (MQ=255)
                 tCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAACTCTCTCTGTCCgtgt                                                         >  2:3185122/1‑76 (MQ=255)
                 tCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTAc                                                       <  1:6394330/76‑1 (MQ=255)
                 tCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGCCCGTGTAc                                                       >  1:1019152/1‑76 (MQ=255)
                  cACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACg                                                      >  1:4515883/1‑76 (MQ=255)
                   aCTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGc                                                     <  2:1917090/76‑1 (MQ=255)
                   aCTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGc                                                     >  1:7070470/1‑76 (MQ=255)
                    cTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGca                                                    >  1:4663259/1‑76 (MQ=255)
                    cTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGca                                                    >  2:7438266/1‑76 (MQ=255)
                     tACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGcac                                                   >  1:7538064/1‑76 (MQ=255)
                     tACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGcac                                                   <  2:2857415/76‑1 (MQ=255)
                      aCGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGcaca                                                  >  1:6942202/1‑76 (MQ=255)
                       cGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAg                                                 <  2:4632328/76‑1 (MQ=255)
                       cGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAg                                                 >  2:1393406/1‑76 (MQ=255)
                       cGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAg                                                 <  2:3202729/76‑1 (MQ=255)
                        ggTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGa                                                <  2:1122687/76‑1 (MQ=255)
                         gTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGAc                                               <  1:7203063/76‑1 (MQ=255)
                          tCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACt                                              <  2:3304923/76‑1 (MQ=255)
                           cgcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTa                                             >  1:2865036/1‑76 (MQ=255)
                           cgcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTa                                             >  1:825480/1‑76 (MQ=255)
                           cgcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTa                                             >  1:4569011/1‑76 (MQ=255)
                           cgcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTa                                             >  1:4260520/1‑76 (MQ=255)
                            gcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTaa                                            >  2:548130/1‑76 (MQ=255)
                            gcgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTaa                                            <  1:7382334/76‑1 (MQ=255)
                             cgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAAc                                           <  1:5133472/76‑1 (MQ=255)
                             cgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAAc                                           >  1:3042383/1‑76 (MQ=255)
                             cgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAAc                                           >  2:3783594/1‑76 (MQ=255)
                             cgTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAAc                                           >  2:593898/1‑76 (MQ=255)
                              gTATGTCCAATCGAAACCCCTGAAGTTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACg                                          <  1:2197316/76‑1 (MQ=255)
                              gTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACg                                          <  2:2667893/76‑1 (MQ=255)
                               tATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGa                                         >  2:1005246/1‑76 (MQ=255)
                                aTGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGaa                                        <  1:976170/76‑1 (MQ=255)
                                aTGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGaa                                        <  1:6047997/76‑1 (MQ=255)
                                aTGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGaa                                        <  2:1520806/76‑1 (MQ=255)
                                aTGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGaa                                        >  2:553101/1‑76 (MQ=255)
                                 tGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAAt                                       >  1:2722461/1‑76 (MQ=255)
                                 tGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAAt                                       <  2:4362819/76‑1 (MQ=255)
                                 tGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAAt                                       >  2:7619502/1‑76 (MQ=255)
                                  gTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATa                                      <  1:6658768/76‑1 (MQ=255)
                                  gTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATa                                      <  1:6500327/76‑1 (MQ=255)
                                   tCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATAc                                     <  1:892811/76‑1 (MQ=255)
                                   tCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATAc                                     <  2:61119/76‑1 (MQ=255)
                                   tCCAATCGAAACCCCTAAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATAc                                     >  2:6961444/1‑76 (MQ=255)
                                    ccAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACg                                    >  2:6317668/1‑76 (MQ=255)
                                    ccAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACg                                    <  2:245163/76‑1 (MQ=255)
                                    ccAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACg                                    >  2:66374/1‑76 (MQ=255)
                                     cAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACgg                                   >  1:414005/1‑76 (MQ=255)
                                     cAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACgg                                   >  2:6082816/1‑76 (MQ=255)
                                        tCGAAACCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCtt                                <  1:7246585/76‑1 (MQ=255)
                                           aaaCCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTTCt                             >  2:3452062/1‑76 (MQ=255)
                                             aCCCCTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTTCttt                           >  2:5305012/1‑75 (MQ=255)
                                              ccccTGAAGGTCCGAACGTCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTga                          <  2:3513635/76‑1 (MQ=255)
                                               cccTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTgag                         >  1:3645970/1‑76 (MQ=255)
                                                ccTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTgaga                        <  2:6025755/76‑1 (MQ=255)
                                                 cTGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGAc                       <  1:538866/76‑1 (MQ=255)
                                                  tGAAGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACt                      <  2:5283278/76‑1 (MQ=255)
                                                    aaGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTcc                    <  1:1208501/76‑1 (MQ=255)
                                                    aaGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTcc                    <  2:7278853/76‑1 (MQ=255)
                                                    aaGGGCCGAACATCGGTCTGACCAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTcc                    <  1:145376/76‑1 (MQ=255)
                                                     aGGTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTTAGACTccc                   >  2:2804503/1‑75 (MQ=255)
                                                      ggTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGAcccccc                  >  1:3197566/1‑74 (MQ=255)
                                                      ggTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGt                  <  2:1211700/76‑1 (MQ=255)
                                                      ggTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGt                  <  2:4015770/76‑1 (MQ=255)
                                                       gTCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTa                 <  1:3299632/76‑1 (MQ=255)
                                                        tCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTAt                <  2:85531/76‑1 (MQ=255)
                                                        tCCGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTAt                <  1:3698896/76‑1 (MQ=255)
                                                         ccGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATc               <  2:7086101/76‑1 (MQ=255)
                                                          cGAACATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTTCTTGAGACTCCCCATCg              >  2:62753/1‑76 (MQ=255)
                                                            aaCATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTTCGACTCCCTATCGTa            >  1:4454811/1‑76 (MQ=255)
                                                            aaCATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTTAGACTCCGTATCATa            >  2:1749955/1‑76 (MQ=255)
                                                            aaCATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTa            <  1:255655/76‑1 (MQ=255)
                                                             aCATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTTCTTTAGAATCCCGCTCGTaa           >  2:2498950/1‑76 (MQ=255)
                                                             aCATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTaa           <  2:5961465/76‑1 (MQ=255)
                                                              cATCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTaaa          <  1:199577/76‑1 (MQ=255)
                                                                tCGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGt        <  1:3917587/76‑1 (MQ=255)
                                                                 cGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTTCTTGAGACTCCCTATCGTAAAGTg       >  2:3567686/1‑76 (MQ=255)
                                                                 cGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTg       <  1:2027141/76‑1 (MQ=255)
                                                                 cGGTCTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTg       >  1:7466394/1‑76 (MQ=255)
                                                                     cTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTTCTTGAGACTCCGTATCCTAAAATGAccc   >  1:2353422/1‑75 (MQ=255)
                                                                     cTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCg   >  1:1744199/1‑76 (MQ=255)
                                                                     cTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCg   <  2:4948690/76‑1 (MQ=255)
                                                                     cTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCg   <  1:1129735/76‑1 (MQ=255)
                                                                     cTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCg   <  1:6241669/76‑1 (MQ=255)
                                                                     cTGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGAACg   >  2:3855230/1‑76 (MQ=255)
                                                                      tGAACAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGa  >  2:5730128/1‑76 (MQ=255)
                                                                         |                                                                          
CGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAA‑‑CTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGA  >  REL606/4162497‑4162642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A