Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F1 I1 R1
|
20 |
54.5 |
3547384 |
95.1% |
3373562 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,165,608 |
(A)8→7 |
coding (1127/2019 nt) |
fadH → |
2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,165,601 | 0 | A | . | 100.0%
| 118.9
/ NA
| 25 | coding (1120/2019 nt) | fadH | 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (14/11); total (14/11) |
GCAAAGTCACCTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCTGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGG > REL606/3165527‑3165671
|
gCAAAGTCCCCTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGaa < 1:763206/76‑1 (MQ=255)
cAAAGTCACCTCGTGCCTGGTGAATCCTCGCGCCTGCCNCGTAACCAAAATGCCAATCCTTCCCGCCGTGCAGaaa < 1:1720861/76‑1 (MQ=255)
aaaGTCACCTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGaaaa > 1:1055605/1‑76 (MQ=255)
aaaGTCACCTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGaaaa > 2:456970/1‑76 (MQ=255)
aaGTCCCCTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGaaaaa < 2:1542049/76‑1 (MQ=255)
tCACCTCGTGGCTGGTGTATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCAGTGCAGaaaaaaat > 1:143158/1‑75 (MQ=255)
aCCTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGaaaaaaatct > 1:1279424/1‑73 (MQ=255)
gTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCgg > 2:1307890/1‑76 (MQ=255)
ggTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGGTGGTCg > 1:478410/1‑76 (MQ=255)
gAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGGTGGTCGGTg > 2:1693155/1‑76 (MQ=255)
aTCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGGTGGTCGGTGCg > 2:1114050/1‑76 (MQ=255)
cTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGGGGGTCGGTGCGGGa > 1:1174737/1‑76 (MQ=255)
gcCTGCCACGAATCGAAAATGCCAATCCTCCCCGCCGTGCTG‑ACAAAAATCTGGCGGTGGTCGGTGCTGGACctgc < 2:35184/76‑1 (MQ=255)
gcCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGGTGGTCGGTGCGGGACctgc > 1:365140/1‑76 (MQ=255)
tGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGGTGGTCGGTGCGGGACCTgctgg < 1:1322601/76‑1 (MQ=255)
gCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGGTGGTCGGTGCGGGACCTgctggg > 2:553317/1‑76 (MQ=255)
ccACGAAACCAAAACGCTACTCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGCTGGCCGGTGCGGGACCTgctgggc < 1:79648/76‑1 (MQ=255)
cACGAAACCAAAATTCCAACGCTTCACGCGGTGCAG‑AAAAAAATCTGGCGTTGGTCGGTGCGGGACCCgctgggct < 2:23939/76‑1 (MQ=255)
cACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGGGGGTCGGGGCGGGACCTgctgggcg > 2:679547/1‑75 (MQ=255)
gAAACCAAAATGCCAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGGTGGTCGGTGCGGGACCTGCTGGGCTGGc < 2:233491/76‑1 (MQ=255)
aaaCCAAAATGCCAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGGTGGTCGGTGCGGGACCTGCTGGGCTGGCg > 2:1350506/1‑76 (MQ=255)
ccAAAATGCCAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGGTGGTCGGTGCGGGACCTGCTGGGCTGGCGttt < 2:1052430/76‑1 (MQ=255)
aaTGCCAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGGTGGTCGGTGCGGGACCTGCTGGGCTGGCGTTTGCCa < 2:1351521/76‑1 (MQ=255)
aaTGCCAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGGTGGTCGGTGCGGGACCTGCTGGGCTGGCGTTTGCCa > 1:1388526/1‑76 (MQ=255)
aaTGCCAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGGTGGTCGGTGCGGGACCTGCTGGGCTGGCGTTTGCCa < 2:657834/76‑1 (MQ=255)
ccAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGGTGGTCGGTGCGGGACCTGCTGGGCTGGCGTTTGCCATTaa < 2:1344368/76‑1 (MQ=255)
ccAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGGTGGTCGGTGCGGGACCTGCTGGGCTGGCGTTTGCCATTaa < 1:367696/76‑1 (MQ=255)
ccAATCCTGCCCGCCGTGCAG‑AAAAAAATCTGGCGGTGGTCGGTGCGGGACCTGCTGGGCTGGCGTTTGCCATTaa < 1:1413778/76‑1 (MQ=255)
cTTCCCGCCGTGAAG‑AAAAAAATCTGGCGGTGGACGGTGCGGGACCTGCTGGGCTGGCGTTNGCCATTAACgcggc > 1:1517397/1‑76 (MQ=255)
ttCCCGCCGTGCAG‑AAAAAAATCTGGTGGTGGTCGGTGCGGGACCTGCTGGGCTGGCGTTTGCCATTAACgcggcg > 2:907657/1‑76 (MQ=255)
cgccgTGCAG‑AAAAAAATCTGGCGGTGGTCGGTGCGGGACCTGCTGGGCTGGCGTTTGCCATTAACGCGGCGgcgc < 2:1613438/76‑1 (MQ=255)
gTGCAG‑AAAAAAATCTGGCGGTGGTCGGTGCGGGACCTGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTgg > 2:491258/1‑76 (MQ=255)
gTGCAG‑AAAAAAATCTGGCGGTGGTCGGTGAGGGACCTGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTgg > 2:1460163/1‑76 (MQ=255)
|
GCAAAGTCACCTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCTGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGG > REL606/3165527‑3165671
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
