Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A27 F1 I1 R1
|
17 |
37.2 |
2333115 |
96.5% |
2251455 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
1,415,090 |
T→C |
H30R (CAC→CGC) |
ydaE ← |
conserved protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 1,415,090 | 0 | T | C | 96.8%
| 119.7
/ ‑3.4
| 31 | H30R (CAC→CGC) | ydaE | conserved protein |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (8/22); total (8/23) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.37e-01 |
ATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTAAGCACAT > REL606/1415017‑1415161
|
aTGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATANTCNCGGTTCGTCTTTTGNTAAAATCCCgcgc > 2:1049897/1‑76 (MQ=255)
gtgCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCAt < 2:1001/76‑1 (MQ=255)
ggTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCACAtgaagtgaag > 1:796647/1‑76 (MQ=255)
tCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCttt < 2:413684/76‑1 (MQ=255)
tCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCttt < 2:413669/76‑1 (MQ=255)
cGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTc > 2:917277/1‑76 (MQ=255)
cGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTc < 2:1078970/76‑1 (MQ=255)
ggCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCa < 1:1008919/76‑1 (MQ=255)
ggCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCa < 1:727029/76‑1 (MQ=255)
gCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCaa < 2:1158062/76‑1 (MQ=255)
acaAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATa < 2:279892/76‑1 (MQ=255)
acaAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCAATa < 1:424832/76‑1 (MQ=255)
aGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCAATAgct > 2:615437/1‑76 (MQ=255)
ttCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCAATAgctttg > 1:44246/1‑76 (MQ=255)
aCTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCAATAgctttgctt > 2:378996/1‑76 (MQ=255)
cTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCAATAgctttgcttt < 2:294574/76‑1 (MQ=255)
tGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCCTTCTTTCAATAGCTTTGCTttc > 2:44149/1‑76 (MQ=255)
cAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTttctt < 2:711361/76‑1 (MQ=255)
aaTATTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTttcttc < 2:1032208/76‑1 (MQ=255)
atatTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCa < 2:673911/76‑1 (MQ=255)
tatTTTCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCaa < 1:199941/76‑1 (MQ=255)
ttttCTCGGTTCGTCTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACg < 2:606653/76‑1 (MQ=255)
ttttCTCGGTTCGTCTTTTGATAAAAGCCCGCGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACg < 2:606639/76‑1 (MQ=255)
cTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAg > 1:276842/1‑76 (MQ=255)
cTTTTGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTNTTTGCAAAg < 1:24289/76‑1 (MQ=255)
ttttGATATAATCCCGCGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTGTTGCAAAgg > 1:42131/1‑76 (MQ=255)
tttGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAggt < 2:651947/76‑1 (MQ=255)
tttGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAggt < 2:651956/76‑1 (MQ=255)
tttGATAAAATCCCGCGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAggt < 2:958067/76‑1 (MQ=255)
aTAAAATCCCGCGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTa < 2:605900/76‑1 (MQ=255)
tCCCGCGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTAAGcaca < 1:213354/76‑1 (MQ=255)
cccGCGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTAAGCACat < 2:476002/76‑1 (MQ=255)
|
ATGTGCCATCTGGTCTTTTTCGGCACAAGCTTCACTGCAATATTTTCTCGGTTCGTCTTTTGATAAAATCCCGTGCATGAAGTGAAGCATTCTTTCAATAGCTTTGCTTTCTTCAACGTCTTTTTTGCAAAGGTGGTAAGCACAT > REL606/1415017‑1415161
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
