Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A47 F1 I1 R1
|
17 |
69.1 |
4502028 |
96.3% |
4335452 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,471,518 |
T→A |
I325N (ATC→AAC) |
yjgQ → |
conserved inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,471,518 | 0 | T | A | 98.4%
| 227.6
/ NA
| 61 | I325N (ATC→AAC) | yjgQ | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (44/16); minor base C (0/1); total (44/17) |
| Fisher's exact test for biased strand distribution p-value = 2.79e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 |
TAGCGTACCGATGGGCGTGCGTGTGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTT > REL606/4471447‑4471590
|
tagcgtaCCGATGGGCGTGCGTGTGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTc > 1:2112048/1‑76 (MQ=255)
agcgtaCCGATGGGCGTGCGTGTGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCg < 1:282006/76‑1 (MQ=255)
cgtaCCGATGGGCGTGCGTGTGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGc > 1:318235/1‑76 (MQ=255)
gtaCCGATGGGCGTGCGTGTGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGcc > 2:1894739/1‑76 (MQ=255)
taCCGATGGGCGTGCGTGTGGTCACCGGTATCAGTTTCGGTTTTTTCTTCTACGTACTGGACCAGAACTTCCGccc > 1:617325/1‑76 (MQ=255)
taCCGATGGGCGTGCGTGTGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAAATTCGGccc > 1:1974562/1‑76 (MQ=255)
aCCGATGGGCGTGCGTGTGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGcccc > 1:1381032/1‑75 (MQ=255)
aCCGATGGGCGTGCGTGTGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCg < 1:593291/76‑1 (MQ=255)
cGATGGGCGTGCGTGTGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCt > 1:3960/1‑76 (MQ=255)
aTGGGCGTGCGTGTGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGa > 1:1962390/1‑76 (MQ=255)
tGGGCGTGCGTGTGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCCCTGAc > 2:1118043/1‑76 (MQ=255)
gcgtgcgtGTGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGtt > 1:2136934/1‑76 (MQ=255)
gcgtgTGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGtt > 2:995882/1‑76 (MQ=255)
gcgtgTGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGtt < 2:536516/76‑1 (MQ=255)
gtgtGGTCACCGGTATCAGTTTCGGTTTTTTCTTCTACGTACTGGACCAGAACTTCGGCCCGCCGCCGTTGGTTTa > 1:987330/1‑76 (MQ=255)
gtgtGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTa < 2:1969631/76‑1 (MQ=255)
gtgtGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTa > 2:1505171/1‑76 (MQ=255)
tgtgGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTAt > 1:100401/1‑76 (MQ=255)
gtgGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCCGACGTTGGTTTTTg > 1:2169349/1‑76 (MQ=255)
ggTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGc > 1:2106409/1‑76 (MQ=255)
gacACCGGTATCAATTTCGGTGTTCTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCa < 1:1283817/74‑1 (MQ=255)
gTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCCGACGTTGGTTTATGGCa > 1:207970/1‑76 (MQ=255)
cACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATc < 2:775496/76‑1 (MQ=255)
aCCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATcc > 2:501306/1‑76 (MQ=255)
aCCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATcc < 2:1803225/76‑1 (MQ=255)
cgttATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCccg < 2:231274/73‑1 (MQ=255)
cGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTTTGGCATcccc > 1:1068682/1‑75 (MQ=255)
cGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCccg > 1:64127/1‑76 (MQ=255)
tATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCccgccg > 1:644644/1‑76 (MQ=255)
tATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCCGACGTTGGTTTATGGCATCcccccg > 1:1891981/1‑76 (MQ=255)
aGTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGatca < 2:1954875/76‑1 (MQ=255)
gTTTCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGatcat > 2:164185/1‑76 (MQ=255)
tttCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGgtcatc > 1:114941/1‑76 (MQ=255)
ttCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGGTCATCg > 1:532345/1‑76 (MQ=255)
ttCGGTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGCTCATCg > 1:957336/1‑76 (MQ=255)
ggTTTTTTCTTCTCCGTACTGGACCAGAACTTCGGCCCGCTGTCGTTGGTTTATGGCATCCCGCCGATCATCGgcg < 1:404898/76‑1 (MQ=255)
ggTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGgcg > 2:364738/1‑76 (MQ=255)
ggTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGgcg > 1:566825/1‑76 (MQ=255)
ggTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGgcg > 1:1736002/1‑76 (MQ=255)
ggTTTTGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGgcg > 1:2059855/1‑76 (MQ=255)
ttttGTCTTCTACGTACTGGACCAGACCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCa < 2:1340342/76‑1 (MQ=255)
ttttGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGgcgcc > 1:279218/1‑75 (MQ=255)
ttttGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCa > 1:1839336/1‑76 (MQ=255)
ttGTCTTCTACCTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACt > 1:781991/1‑76 (MQ=255)
tGTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTg > 1:1023732/1‑76 (MQ=255)
gTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGt > 1:1823432/1‑76 (MQ=255)
gTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGt > 1:2112853/1‑76 (MQ=255)
gTCTTCTACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGt > 1:2153001/1‑76 (MQ=255)
tcttctACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGtt < 1:1802507/76‑1 (MQ=255)
tctACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGcc > 1:10469/1‑76 (MQ=255)
tctACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGcc > 2:870503/1‑76 (MQ=255)
ctACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCa < 2:200484/76‑1 (MQ=255)
tACGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCaa > 1:1957217/1‑76 (MQ=255)
aCGTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAg > 1:1785512/1‑76 (MQ=255)
gTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAgcg > 2:1930576/1‑76 (MQ=255)
gTACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAgcg < 1:833128/76‑1 (MQ=255)
tACTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCCAgcgc > 1:1215886/1‑76 (MQ=255)
aCTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGcc < 2:1035709/76‑1 (MQ=255)
cTGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCa < 1:2119082/76‑1 (MQ=255)
tGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAg > 1:1294491/1‑76 (MQ=255)
tGGACCAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCCGCGCACTGTTGCCAAGCGCCAg > 1:865043/1‑76 (MQ=255)
ccAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGcttc < 2:2139497/76‑1 (MQ=255)
ccAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGcttc < 2:69525/76‑1 (MQ=255)
ccAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCCGCGCACTGTTGCCCAGCGCCAGcttc > 2:931721/1‑76 (MQ=255)
cAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGcttct > 2:1189524/1‑76 (MQ=255)
cAGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGcttct < 1:648533/76‑1 (MQ=255)
aGAACTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGcttctt > 1:61378/1‑76 (MQ=255)
aGAACTTCGGCCCGCTGACGTTAGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGcttctt > 1:2219852/1‑76 (MQ=255)
|
TAGCGTACCGATGGGCGTGCGTGTGGTCACCGGTATCAGTTTCGGTTTTGTCTTCTACGTACTGGACCAGATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTT > REL606/4471447‑4471590
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
