Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A60 F1 I1 R1
|
14 |
61.1 |
3976207 |
96.5% |
3837039 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,665,436 |
(A)7→6 |
coding (405/483 nt) |
yfiB → |
predicted outer membrane lipoprotein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,665,430 | 0 | A | . | 100.0%
| 157.1
/ NA
| 32 | coding (399/483 nt) | yfiB | predicted outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (20/12); total (20/12) |
GGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGAT > REL606/2665355‑2665501
|
ggCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGa > 1:1801455/1‑76 (MQ=255)
gCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGaa < 2:1059198/76‑1 (MQ=255)
cGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGaaa > 2:1317861/1‑76 (MQ=255)
cGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGaaa < 1:935393/76‑1 (MQ=255)
gAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGaaaa > 1:1235467/1‑76 (MQ=255)
gAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGaaaa > 1:488550/1‑76 (MQ=255)
aaTGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGTaaaaa > 2:55546/1‑76 (MQ=255)
aaTGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGaaaaa > 1:817232/1‑76 (MQ=255)
aaTGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGaaaaa > 2:1769140/1‑76 (MQ=255)
aaTGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGaaaaa > 2:90610/1‑76 (MQ=255)
tGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGaaaaaat < 1:1911796/76‑2 (MQ=255)
gTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGaaaaanta < 2:1395422/76‑4 (MQ=255)
gTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGaaaaaata < 1:1696882/76‑3 (MQ=255)
tCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGaaaaaatat < 1:1809333/76‑4 (MQ=255)
ggCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCa < 1:1648128/76‑1 (MQ=255)
cGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAg > 2:1834681/1‑76 (MQ=255)
gATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTGACCACACAGGGTTTAGG‑AAAAAATATCCCATAGc < 1:525455/76‑1 (MQ=255)
gATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGc > 1:759985/1‑76 (MQ=255)
aTGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGcc > 1:1900441/1‑76 (MQ=255)
aTGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTa < 2:10014/76‑1 (MQ=255)
aTGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTa > 1:72237/1‑76 (MQ=255)
cTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAg < 2:1021173/76‑1 (MQ=255)
aCAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAgg > 1:310235/1‑76 (MQ=255)
cAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAggg < 1:1670621/76‑1 (MQ=255)
aaaTTCCACGCAGCAATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGc > 2:925252/1‑76 (MQ=255)
aTTCCACGCAGCAATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCcg > 2:458493/1‑76 (MQ=255)
ttCCACGCAGCAATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCcgc > 1:1086328/1‑76 (MQ=255)
aCGCAGCAATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCg < 1:1669832/76‑1 (MQ=255)
gcagcaATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCgag > 2:1056938/1‑76 (MQ=255)
cagcaATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCgaga < 2:1097654/76‑1 (MQ=255)
agcaATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGaa > 2:678824/1‑76 (MQ=255)
agcaATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGaa < 1:583571/76‑1 (MQ=255)
gcaATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAAc < 1:1558537/76‑1 (MQ=255)
caATCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAAcc > 1:1481481/1‑76 (MQ=255)
aaTCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAAccg > 1:892677/1‑76 (MQ=255)
aTCTCACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAAccgc > 1:1267323/1‑76 (MQ=255)
tctcACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAAccgcc > 1:271618/1‑76 (MQ=255)
tctcACCACACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAAccgcc > 2:1575272/1‑76 (MQ=255)
caccacACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGCCCGCCCAGGGCCGCGCCGAGAACCGCcgcg < 2:1631859/76‑1 (MQ=255)
caccacACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCcgcg < 1:114630/76‑1 (MQ=255)
caccacACAGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCcgcg > 1:848001/1‑76 (MQ=255)
ccacACGGGGTTAAGG‑AAAAAATACCCCATAGCCAGTAACAAGACCGCCCGGGGCCGCGCCGAGAACCGCCGCGTc < 1:649400/76‑1 (MQ=255)
aGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAgtg > 1:18673/1‑76 (MQ=255)
aGGGTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAgcg > 1:1532803/1‑74 (MQ=255)
gTTTAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAgtggtg < 1:730049/76‑1 (MQ=255)
tttAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGa > 2:1898825/1‑76 (MQ=255)
ttAGG‑AAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGAt > 2:788347/1‑76 (MQ=255)
|
GGCGAATGTCGTGGCCGATGCATGGGCTATGGGTGGACAAATTCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGAT > REL606/2665355‑2665501
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
