Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A101 F1 I1 R1 20 160.9 10398134 92.8% 9649468 75.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 2,060,017 C→T G127S (GGC→AGC)  wcaG ← bifunctional GDP‑fucose synthetase: GDP‑4‑dehydro‑6‑deoxy‑D‑mannose epimerase/ GDP‑4‑dehydro‑6‑L‑deoxygalactose reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6062,060,0170CT98.0% 370.2 / ‑4.1 101G127S (GGC→AGC) wcaGbifunctional GDP‑fucose synthetase: GDP‑4‑dehydro‑6‑deoxy‑D‑mannose epimerase/ GDP‑4‑dehydro‑6‑L‑deoxygalactose reductase
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base T (34/65);  total (36/65)
Fisher's exact test for biased strand distribution p-value = 1.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 115 total aligned reads displayed.

TTGTAAGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCCCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACGATCCGAGAAACA  >  REL606/2059944‑2060088
                                                                         |                                                                        
ttGTAAGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGctct                                                                        <  1:5109703/76‑1 (MQ=255)
 tGTAAGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTg                                                                       <  2:1443821/76‑1 (MQ=255)
 tGTAAGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTg                                                                       <  2:193118/76‑1 (MQ=255)
 tGTAAGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTg                                                                       <  2:3715218/76‑1 (MQ=255)
  gTAAGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGctcttc                                                                      >  1:4821441/1‑74 (MQ=255)
  gTAAGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGc                                                                      >  1:2959896/1‑76 (MQ=255)
  gTAAGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGc                                                                      <  2:1056456/76‑1 (MQ=255)
  gTAAGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGc                                                                      <  2:1129873/76‑1 (MQ=255)
  gTAAGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGc                                                                      >  1:3514407/1‑76 (MQ=255)
  gTAAGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGc                                                                      >  2:4440087/1‑76 (MQ=255)
   tAAGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGca                                                                     <  1:1565950/76‑1 (MQ=255)
    aaGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGcaa                                                                    <  1:2002541/76‑1 (MQ=255)
    aaGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGcaa                                                                    >  2:2277019/1‑76 (MQ=255)
     aGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGcaac                                                                   >  2:4036504/1‑76 (MQ=255)
     aGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGcaac                                                                   <  1:2428682/76‑1 (MQ=255)
      gATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGcaaca                                                                  <  1:3068364/76‑1 (MQ=255)
       aTTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGcaacaa                                                                 <  2:4431252/76‑1 (MQ=255)
       aTTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGcaacaa                                                                 >  2:3540231/1‑76 (MQ=255)
        ttCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCaacaac                                                                >  2:1754214/1‑76 (MQ=255)
         tCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACt                                                               >  1:1033500/1‑76 (MQ=255)
         tCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACt                                                               >  2:1620130/1‑76 (MQ=255)
              caGTTTGATCCTGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGGGCTCTGCAACAACTCGCtt                                                          >  1:135987/1‑76 (MQ=255)
              caGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCtt                                                          <  2:3708006/76‑1 (MQ=255)
              caGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCtt                                                          >  1:4117467/1‑76 (MQ=255)
               aGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCttt                                                         >  2:2062999/1‑76 (MQ=255)
               aGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCttt                                                         <  2:4669734/76‑1 (MQ=255)
                gTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTc                                                        <  1:3124382/76‑1 (MQ=255)
                 tttGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCt                                                       >  2:1161162/1‑76 (MQ=255)
                  ttGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTg                                                      >  1:1040552/1‑76 (MQ=255)
                   tGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCCGCGTGCTCCGCAACAACTCGCTTTCCGc                                                     >  1:4631677/1‑76 (MQ=255)
                   tGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGc                                                     >  1:357344/1‑76 (MQ=255)
                   tGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGc                                                     >  1:4363966/1‑76 (MQ=255)
                    gATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGcc                                                    >  1:3436295/1‑76 (MQ=255)
                    gATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGcc                                                    <  2:2560340/76‑1 (MQ=255)
                       cccGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATc                                                 >  2:3779974/1‑76 (MQ=255)
                       cccGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATc                                                 <  1:1583036/76‑1 (MQ=255)
                       cccGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATc                                                 <  1:4379992/76‑1 (MQ=255)
                       cccGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATc                                                 >  2:3636516/1‑76 (MQ=255)
                        ccGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCg                                                >  2:2762652/1‑76 (MQ=255)
                         cagCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCgg                                               >  1:42070/3‑76 (MQ=255)
                          ggCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGc                                              <  2:4987454/76‑1 (MQ=255)
                           gCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCCCTGCAACAACTCGCTTTCTGCCATCGGCt                                             >  1:976717/1‑76 (MQ=255)
                             gATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑Gt                                          <  1:3911836/76‑1 (MQ=255)
                             gATTTTGGCAATAGCATAAGGCTCGTTAGTCAGCTCCAGCGAGCTCTGAAACAACTCGCTTTCTGGCATCGGCT‑gg                                          >  1:405244/1‑75 (MQ=255)
                               ttttGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCCTCGGCT‑gggt                                        >  1:1809986/1‑73 (MQ=255)
                               ttttGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑gggt                                        >  2:653752/1‑73 (MQ=255)
                               ttttGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑Gttt                                        <  1:5029003/76‑1 (MQ=255)
                               ttttGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑Gttt                                        <  1:2277019/76‑1 (MQ=255)
                                tttGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑Gtttt                                       <  2:574604/76‑1 (MQ=255)
                                tttGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑Gtttt                                       <  2:2654707/76‑1 (MQ=255)
                                 ttGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTg                                      <  1:1345452/76‑1 (MQ=255)
                                 ttGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTg                                      <  2:1406729/76‑1 (MQ=255)
                                   ggCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGcc                                    <  2:4177867/76‑1 (MQ=255)
                                     cAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GGTTTGcctt                                  >  1:5048041/1‑74 (MQ=255)
                                     cAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCCGCT‑GGTTTGCCAg                                  >  1:4578486/1‑76 (MQ=255)
                                        tAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCTGGTTTTGCCGGtt                                >  1:1513910/1‑76 (MQ=255)
                                        tAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGttt                               <  2:2492772/76‑1 (MQ=255)
                                         aGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGTT‑GTTTTGCCAGTTTc                              <  2:4332264/76‑1 (MQ=255)
                                         aGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCTGGTTTTTCCGGttt                               >  2:2135125/1‑76 (MQ=255)
                                         aGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTc                              <  1:1139947/76‑1 (MQ=255)
                                         aGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTc                              <  2:4621668/76‑1 (MQ=255)
                                          gCATAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCg                             <  1:121233/76‑1 (MQ=255)
                                             tAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGt                          <  1:1437211/76‑1 (MQ=255)
                                             tAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGt                          <  1:547606/76‑1 (MQ=255)
                                             tAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGt                          <  2:4036628/76‑1 (MQ=255)
                                             tAAGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGt                          <  1:4876345/76‑1 (MQ=255)
                                              aaGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTa                         <  1:2907583/76‑1 (MQ=255)
                                               aGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAg                        <  2:2977718/76‑1 (MQ=255)
                                               aGGCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAg                        <  1:656359/76‑1 (MQ=255)
                                                 gCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGAt                      <  1:2924108/76‑1 (MQ=255)
                                                 gCTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GGTTTGTCCGTTTCGGGgagaa                      >  1:4562173/1‑75 (MQ=255)
                                                  cTCGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATa                     <  2:199555/76‑1 (MQ=255)
                                                    cGTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GGTTTGCCAGTTTTTGGGAGAGACa                   >  2:4886136/1‑76 (MQ=255)
                                                     gTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAg                  <  1:653752/76‑1 (MQ=255)
                                                     gTTAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAg                  <  1:3238553/76‑1 (MQ=255)
                                                     gTTAGTCGGCTCCAGCGTGCTCGGCAACACCTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAg                  <  1:47151/76‑1 (MQ=255)
                                                     gTTAGGCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAg                  <  2:2992344/76‑1 (MQ=255)
                                                      ttAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAgg                 <  1:2931248/76‑1 (MQ=255)
                                                      ttAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAgg                 <  1:562975/76‑1 (MQ=255)
                                                       tAGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGa                <  2:1754916/76‑1 (MQ=255)
                                                        aGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GGTTTTTCAGTTTTGGGGGGATAAAGGAc               >  1:3342551/1‑76 (MQ=255)
                                                        aGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GGTTTTGCAGCTTTGGGGAAATACAGGAc               >  1:3409645/1‑76 (MQ=255)
                                                        aGTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGACATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGAc               <  1:2657559/76‑1 (MQ=255)
                                                         ggcGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACg              <  2:2931571/74‑1 (MQ=255)
                                                         gTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACg              <  1:887709/76‑1 (MQ=255)
                                                         gTCGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACg              <  1:1620279/76‑1 (MQ=255)
                                                           cGGCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACGAt            <  2:119846/76‑1 (MQ=255)
                                                            ggCTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACGATc           <  1:2209350/76‑1 (MQ=255)
                                                              cTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACGATCCg         <  2:152102/76‑1 (MQ=255)
                                                              cTCCAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACGATCCg         <  1:1638064/76‑1 (MQ=255)
                                                                ccAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACGATCCgag       <  2:39239/76‑1 (MQ=255)
                                                                ccAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GGTTTGTCCGTTTCCGGTGGGAACAGGGCGGTCCcag       >  2:1842624/1‑76 (MQ=255)
                                                                 cAGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACGATCCgaga      <  1:3697463/76‑1 (MQ=255)
                                                                  aGCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACGATCCGAGaa     <  1:3111110/76‑1 (MQ=255)
                                                                   gCGTGCTCTGCAACACCTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACGATCCGAGaaa    <  1:1629757/76‑1 (MQ=255)
                                                                   gCGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACGATCCGAGaaa    <  1:4650494/76‑1 (MQ=255)
                                                                    ggTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACGATCCGAGAAAc   <  2:3385967/75‑1 (MQ=255)
                                                                    cGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACGATCCGAGAAAc   <  2:3363830/76‑1 (MQ=255)
                                                                    cGTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACGATCCGAGAAAc   <  2:2869548/76‑1 (MQ=255)
                                                                     gTGCTCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACGATCCGAGAAAca  <  1:1696448/76‑1 (MQ=255)
                                                                         |                                                                        
TTGTAAGATTCGCACAGTTTGATCCCGGCGATTTTGGCAATAGCATAAGGCTCGTTAGTCGGCTCCAGCGTGCCCTGCAACAACTCGCTTTCTGCCATCGGCT‑GTTTTGCCAGTTTCGGGTAGATACAGGACGATCCGAGAAACA  >  REL606/2059944‑2060088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A