mutation predictions | marginal predictions | summary statistics | genome diff | command line log
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breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| Predicted mutations | |||||
|---|---|---|---|---|---|
| evidence | position | mutation | annotation | gene | description |
| RA | 474,629 | A→G | L226P (CTG→CCG) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| RA | 500,385 | T→G | D90E (GAT→GAG) | ylbH → | conserved protein, rhs‑like |
| RA | 649,574 | T→C | K410E (AAA→GAA) | mrdA ← | transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2) |
| RA | 796,336 | (T)8→7 | intergenic (‑7/‑419) | ECB_00737 ← / → ECB_00738 | hypothetical protein/hypothetical protein |
| RA | 1,290,813 | C→T | Q260* (CAG→TAG) | hnr → | response regulator of RpoS |
| RA | 1,329,516 | C→T | H33Y (CAC→TAC) | topA → | DNA topoisomerase I |
| JC | 1,733,297 | (CGTATGAA)1→2 | coding (333/1413 nt) | pykF → | pyruvate kinase |
| RA | 2,060,017 | C→T | G127S (GGC→AGC) | wcaG ← | bifunctional GDP‑fucose synthetase: GDP‑4‑dehydro‑6‑deoxy‑D‑mannose epimerase/ GDP‑4‑dehydro‑6‑L‑deoxygalactose reductase |
| RA | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
| RA | 2,519,900 | C→A | R230L (CGC→CTC) | dapA ← | dihydrodipicolinate synthase |
| JC | 3,014,094 | (ACAGGC)3→2 | coding (675‑680/1137 nt) | ECB_02815 → | Capsule polysaccharide export inner‑membrane protein kpsE |
| RA | 3,145,750 | C→T | intergenic (+81/‑114) | dnaG → / → rpoD | DNA primase/RNA polymerase sigma factor |
| RA | 3,285,721 | A→C | intergenic (‑27/+203) | yhbL ← / ← arcB | isoprenoid biosynthesis protein with amidotransferase‑like domain/hybrid sensory histidine kinase in two‑component regulatory system with ArcA |
| RA | 3,341,748 | A→G | intergenic (‑392/‑7) | envR ← / → acrE | DNA‑binding transcriptional regulator/cytoplasmic membrane lipoprotein |
| RA | 3,762,741 | A→T | K662I (AAA→ATA) | spoT → | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
| RA | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
| MC JC | 3,894,997 | Δ6,934 bp | IS150‑mediated | rbsD–[yieO] | rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO] |
| RA | 3,911,972 | (T)7→8 | intergenic (‑173/‑150) | yifB ← / → ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| RA | 4,162,513 | C→T | T553I (ACT→ATT) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| JC JC | 4,524,522 | IS186 (+) +6 bp | coding (494‑499/549 nt) | fimA → | major type 1 subunit fimbrin (pilin) |
| Unassigned missing coverage evidence | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | REL606 | 546975–547700 | 593358 | 45659–46384 | 64 [57] | [58] 60 | [insB‑6]–[fepA] | [insB‑6],insA‑6,nmpC,ybcR,ybcS,ybcT,ybcU,ECB_00510,nohB,ECB_00512,ECB_00513,ECB_00514,ECB_00515,ECB_00516,ECB_00517,appY,ompT,envY,ybcH,nfrA,nfrB,nfrB,ECB_00524,yhhI,ECB_00526,ECB_00527,ECB_00528,ECB_00529,ECB_00530,cusS,cusR,cusC,ylcC,cusB,cusA,pheP,ybdG,nfnB,ybdF,ybdJ,ybdK,insJ‑1,insK‑1,insB‑7,insA‑7,insK‑1,hokE,insL‑3,entD,[fepA] |
| * | * | ÷ | REL606 | 593583 | 619843–619116 | 25534–26261 | 59 [57] | [57] 60 | [fepA]–insA‑8 | [fepA],fes,ybdZ,entF,fepE,fepC,fepG,fepD,ybdA,fepB,entC,entE,entB,entA,ybdB,cstA,cstA,ybdD,ybdH,ybdL,ybdM,ybdN,insB‑8,insA‑8 |