breseq  version 0.33.1  revision 8505477f25b3 mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 191 shown, sorted by frequency from high to low)
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,515,909	0	A	C	57.1%	17.3 / 58.6	42	V260G (GTG→GGG)	ypfI	predicted hydrolase
*	REL606	4,180,249	0	T	G	48.8%	39.5 / 14.9	43	G24G (GGT→GGG)	yjaH	hypothetical protein
*	REL606	433	0	T	G	46.6%	63.5 / 32.4	58	V33G (GTG→GGG)	thrA	bifunctional aspartokinase I/homeserine dehydrogenase I
*	REL606	1,747,903	0	G	T	43.0%	304.0 / inf	291	A8S (GCA→TCA)	ydiL	hypothetical protein
*	REL606	2,304,407	0	C	A	41.9%	71.6 / 83.5	74	Q266K (CAA→AAA)	yfaD	hypothetical protein
*	REL606	4,392,556	0	C	T	41.8%	90.5 / 72.6	91	L43L (CTA→TTA)	aidB	isovaleryl CoA dehydrogenase
*	REL606	3,378,885	0	T	G	41.1%	246.1 / 55.6	141	T129P (ACC→CCC)	rplB	50S ribosomal protein L2
*	REL606	1,854,401	0	A	C	40.8%	64.7 / 16.8	50	K88N (AAA→AAC)	yeaO	hypothetical protein
*	REL606	3,472,700	0	C	G	39.3%	53.1 / 42.1	56	A290G (GCG→GGG)	yhgA	predicted transposase
*	REL606	1,118,209	0	G	T	39.1%	33.1 / 39.1	46	intergenic (‑444/‑311)	csgD/csgB	DNA‑binding transcriptional activator in two‑component regulatory system/curlin nucleator protein, minor subunit in curli complex
*	REL606	2,171,670	0	A	G	38.3%	77.4 / 35.0	60	S520P (TCC→CCC)	bglX	beta‑D‑glucoside glucohydrolase, periplasmic
*	REL606	3,774,914	0	C	G	38.3%	175.1 / 109.6	120	A20P (GCC→CCC)	yicJ	predicted transporter
*	REL606	2,376,348	0	T	G	37.5%	144.3 / 17.9	89	T63P (ACC→CCC)	folC	bifunctional folylpolyglutamate synthase/ dihydrofolate synthase
*	REL606	159,654	0	A	C	36.1%	350.9 / 27.8	169	G27G (GGT→GGG)	yadN	predicted fimbrial‑like adhesin protein
*	REL606	3,783,565	0	A	C	36.0%	150.9 / 34.4	89	T744P (ACA→CCA)	ECB_03521	conserved hypothetical protein
*	REL606	2,730,462	0	C	G	35.7%	29.1 / 10.5	28	A608A (GCG→GCC)	hypF	carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases
*	REL606	2,044,357	0	A	C	35.3%	223.0 / 64.6	119	V284G (GTG→GGG)	rfbD	dTDP‑4‑dehydrorhamnose reductase subunit, NAD(P)‑binding, of dTDP‑L‑rhamnose synthase
*	REL606	3,096,354	0	A	C	34.9%	131.4 / 39.4	83	V163G (GTG→GGG)	sufI	repressor protein for FtsI
*	REL606	1,485,450	0	A	G	34.3%	238.7 / 91.7	137	G193G (GGA→GGG)	ydcV	predicted spermidine/putrescine transporter subunit
*	REL606	955,035	0	A	C	34.0%	206.8 / 10.2	94	T192P (ACC→CCC)	lolA	outer‑membrane lipoprotein carrier protein precursor

Marginal new junction evidence (sorted from low to high skew)
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	312594 =	234 (1.490)	77 (0.490)	10/150	10.4	21.0%	intergenic (+202/‑220)	yahG/yahI	hypothetical protein/predicted carbamate kinase‑like protein
	?	REL606	= 2583282	345 (2.190)					coding (741/741 nt)	yfhQ	predicted methyltransferase
*	?	REL606	281214 =	176 (1.120)	5 (0.030)	3/138	13.6	2.9%	coding (304/591 nt)	ykgK	predicted regulator
	?	REL606	281564 =	170 (1.180)					intergenic (‑47/‑728)	ykgK/ykgL	predicted regulator/hypothetical protein
*	?	REL606	= 2105023	180 (1.140)	8 (0.050)	3/148	14.1	4.2%	coding (1103/1119 nt)	ECB_01993	hypothetical protein
	?	REL606	= 2105232	187 (1.210)					intergenic (+193/+19)	ECB_01993/ECB_01994	hypothetical protein/hypothetical protein