Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A101 F1 I1 R1
|
20 |
160.9 |
10398134 |
92.8% |
9649468 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,162,513 |
C→T |
T553I (ACT→ATT) |
rpoB → |
DNA‑directed RNA polymerase subunit beta |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,162,513 | 0 | C | T | 99.4%
| 682.3
/ ‑4.7
| 169 | T553I (ACT→ATT) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (92/76); total (93/76) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 |
Only 100 of 179 total aligned reads displayed.
CGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGT > REL606/4162440‑4162585
|
cgtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTc > 2:3296833/1‑76 (MQ=255)
cgtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTc < 2:2439057/76‑1 (MQ=255)
gtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCa < 2:2625654/76‑1 (MQ=255)
tATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCAc > 1:3888197/1‑76 (MQ=255)
aTCTCCTCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACt < 1:4969950/76‑1 (MQ=255)
aTCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACCCCCGATTCACt > 1:173158/1‑76 (MQ=255)
aTCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACt > 2:4885104/1‑76 (MQ=255)
aTCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACt > 1:3509988/1‑76 (MQ=255)
aTCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACt > 2:2323561/1‑76 (MQ=255)
tctcCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTa > 1:1507348/1‑76 (MQ=255)
tctcCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTa < 2:201570/76‑1 (MQ=255)
tctcCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTa < 1:3662987/76‑1 (MQ=255)
tctcCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTa > 2:3135477/1‑76 (MQ=255)
ctcCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTAc < 2:2496935/76‑1 (MQ=255)
tcCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACg > 2:4936255/1‑76 (MQ=255)
ccGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACgg > 2:200691/1‑76 (MQ=255)
ccGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACgg > 2:4360530/1‑76 (MQ=255)
ccGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACgg < 1:4698947/76‑1 (MQ=255)
ccGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTAAgg > 2:2908744/1‑76 (MQ=255)
cACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTcg < 1:397704/76‑1 (MQ=255)
cACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTcg < 1:4271596/76‑1 (MQ=255)
cACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTcg < 2:221096/76‑1 (MQ=255)
cACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTcg > 2:386823/1‑76 (MQ=255)
cACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTcg < 1:2079802/76‑1 (MQ=255)
tCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGt > 2:748842/1‑76 (MQ=255)
ggCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTAt > 2:4106994/1‑76 (MQ=255)
ggCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTAt > 2:2182735/1‑76 (MQ=255)
ggCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTAt < 1:3913251/76‑1 (MQ=255)
ggCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTAt > 2:4643680/1‑76 (MQ=255)
ggCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTAt < 2:3943558/76‑1 (MQ=255)
gCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATg > 1:926431/1‑76 (MQ=255)
gCCAAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTCCGAGACGTACACCCGATTCACTACGGTCGCGTATg < 1:293228/76‑1 (MQ=255)
ccAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTc < 2:708800/76‑1 (MQ=255)
ccAGGCGGTCTGACCCGCGAACGTGCAGGCTTCGGCGTTCGCGACGTACACCCGATTCACTACGCTCGCGAATGTc > 2:3524365/1‑76 (MQ=255)
cAGGCTGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTcc < 2:4752380/76‑1 (MQ=255)
cAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTcc > 2:2939333/1‑76 (MQ=255)
cAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTcc < 2:1882296/76‑1 (MQ=255)
cAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTcc > 2:241282/1‑76 (MQ=255)
cAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTcc < 1:2110310/76‑1 (MQ=255)
aGGCGGTCTGTCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCa < 1:3402581/76‑1 (MQ=255)
aGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCa < 2:3066665/76‑1 (MQ=255)
gCGGTCTGCCCCGTGAACGTGCAGGCTTCGAGGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAAt < 1:4518246/76‑1 (MQ=255)
gCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAAt < 1:2052046/76‑1 (MQ=255)
cGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATc < 2:811130/76‑1 (MQ=255)
cGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATc < 2:3570719/76‑1 (MQ=255)
ggTCTGACCCGTGAGCGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCg > 1:4134038/1‑76 (MQ=255)
ggTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCg > 2:3439359/1‑76 (MQ=255)
gTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGa > 1:1254598/1‑76 (MQ=255)
gTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGa > 2:4455706/1‑76 (MQ=255)
cTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGaaa > 2:3179141/1‑76 (MQ=255)
cTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACCACGGTGGCGTATGTCCGATGGaaa > 1:4412811/1‑76 (MQ=255)
tGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAAc < 2:3836387/76‑1 (MQ=255)
tGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAAc > 1:84490/1‑76 (MQ=255)
gACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAAcc > 1:1812615/1‑76 (MQ=255)
gACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAAcc > 2:4808882/1‑76 (MQ=255)
gACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAAcc > 1:1157054/1‑76 (MQ=255)
ccGTGAACGTGCAGGCTTCGAAGTTTGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCt < 1:1272523/76‑1 (MQ=255)
ccGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCt < 1:1827474/76‑1 (MQ=255)
gTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGa < 2:4200073/76‑1 (MQ=255)
gTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGa < 1:3315876/76‑1 (MQ=255)
tGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGac > 2:1251481/1‑75 (MQ=255)
tGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGaa < 2:4556910/76‑1 (MQ=255)
tGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGaa < 1:4582608/76‑1 (MQ=255)
gAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAg > 1:2731235/1‑76 (MQ=255)
aaCGTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAgg > 1:4186324/1‑76 (MQ=255)
gTGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTcc > 2:3633573/1‑76 (MQ=255)
tGCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCg > 1:3247503/1‑76 (MQ=255)
gCAGGTTTCGAAGTCCGAGCCGTCCACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGa < 1:2759496/76‑1 (MQ=255)
gCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGa > 1:4632475/1‑76 (MQ=255)
gCAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGa > 1:656226/1‑76 (MQ=255)
cAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGaa < 1:3688427/76‑1 (MQ=255)
cAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGaa > 2:4627804/1‑76 (MQ=255)
cAGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGaa > 1:286550/1‑76 (MQ=255)
aGGCTTCGAAGTTCGAGACGTACGCCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAAc < 1:1035966/76‑1 (MQ=255)
aGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAAc < 1:2401405/76‑1 (MQ=255)
aGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAAc < 2:2539168/76‑1 (MQ=255)
aGGCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAAc < 1:1185407/76‑1 (MQ=255)
aGGCTTCAAAGTGCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAAc < 1:4798099/76‑1 (MQ=255)
gCTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACAt > 2:2183924/1‑76 (MQ=255)
cTTCGAAGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATc > 1:2171685/1‑76 (MQ=255)
aaGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCt > 2:1481523/1‑76 (MQ=255)
aaGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCt < 1:106115/76‑1 (MQ=255)
aaGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCt > 2:2847811/1‑76 (MQ=255)
aGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTg < 2:4208988/76‑1 (MQ=255)
aGTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTg > 2:1579340/1‑76 (MQ=255)
aGTGCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTg < 1:160297/76‑1 (MQ=255)
gTTCGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGa > 1:1951387/1‑76 (MQ=255)
cGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCa > 1:4839070/1‑76 (MQ=255)
cGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCa < 1:3244917/76‑1 (MQ=255)
cGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCa < 2:1861052/76‑1 (MQ=255)
cGAGACGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCa < 2:2748249/76‑1 (MQ=255)
cGAGACGGACACCGGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCa < 1:4092924/76‑1 (MQ=255)
agaCGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAAc > 2:4316244/1‑76 (MQ=255)
aCGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAActc > 2:2131754/1‑76 (MQ=255)
aCGTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAActc > 1:1981344/1‑76 (MQ=255)
gTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAActctc < 2:3740171/76‑1 (MQ=255)
gTACACCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAActctc < 2:4930497/76‑1 (MQ=255)
acacCCGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTg > 1:3172219/1‑76 (MQ=255)
cccGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTcc < 1:3933278/76‑1 (MQ=255)
cccGATTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTcc > 2:3526111/1‑76 (MQ=255)
|
CGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGT > REL606/4162440‑4162585
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
