Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A138 F1 I1 R1
|
17 |
163.5 |
10262604 |
95.7% |
9821312 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,163,127 |
C→T |
R758C (CGT→TGT) |
rpoB → |
DNA‑directed RNA polymerase subunit beta |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,163,127 | 0 | C | T | 98.7%
| 631.1
/ ‑1.5
| 154 | R758C (CGT→TGT) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (91/61); total (92/62) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01 |
Only 100 of 155 total aligned reads displayed.
TATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACG > REL606/4163054‑4163199
|
tATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAGCCTGACCAAATACACCTGt > 1:1192775/1‑76 (MQ=255)
tATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGt > 1:3956248/1‑76 (MQ=255)
tATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGt < 1:3030523/76‑1 (MQ=255)
aTCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGtt > 2:3501633/1‑76 (MQ=255)
aTCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGtt > 1:4502274/1‑76 (MQ=255)
aTCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGtt > 1:3006935/1‑76 (MQ=255)
aTCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGtt > 1:703317/1‑76 (MQ=255)
cAAAGTTAACGAAGACGAGATGTATCCGTGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCt < 2:2869588/76‑1 (MQ=255)
cAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCt < 2:4981133/76‑1 (MQ=255)
cAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCt < 2:2706840/76‑1 (MQ=255)
cAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCt > 2:2091969/1‑76 (MQ=255)
aaaGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTa > 1:4262222/1‑76 (MQ=255)
aaaGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTa < 2:3947991/76‑1 (MQ=255)
aaaGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTa < 2:3300601/76‑1 (MQ=255)
aaaGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTa < 1:1449106/76‑1 (MQ=255)
aGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAAc > 1:1912704/1‑76 (MQ=255)
aGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAAc < 2:5111847/76‑1 (MQ=255)
aGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAAc > 2:1176772/1‑76 (MQ=255)
gTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAAcc < 1:2421043/76‑1 (MQ=255)
gTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAAcc > 1:3331735/1‑76 (MQ=255)
tAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAg > 2:2845190/1‑76 (MQ=255)
aaCGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGa < 1:4659263/76‑1 (MQ=255)
aaCGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGa > 1:4561542/1‑76 (MQ=255)
cGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAac > 2:1787511/1‑76 (MQ=255)
gAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAaca > 1:3926854/1‑76 (MQ=255)
gAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAaca > 2:2780663/1‑76 (MQ=255)
aaGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAacac > 2:525875/1‑76 (MQ=255)
aaGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAacac < 1:3118122/76‑1 (MQ=255)
aGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACAcc > 2:1219205/1‑76 (MQ=255)
gACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACAcca < 2:1377069/76‑2 (MQ=255)
gataTGCATCCGGATGAAGCAGGTATCGACATCTACATCCTGACCAAATACCCCTGTTCTAACCAGAACACCTTTa < 2:1347659/73‑1 (MQ=255)
gagaTGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTa > 2:3084689/1‑76 (MQ=255)
gagaTGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTa > 2:2527127/1‑76 (MQ=255)
gagaTGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTa > 2:2439852/1‑76 (MQ=255)
gaTGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATc < 1:3973505/76‑1 (MQ=255)
aTGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCa < 1:4413575/76‑1 (MQ=255)
aTGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCa < 1:103998/76‑1 (MQ=255)
tGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCaa < 2:253540/76‑1 (MQ=255)
tGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCaa > 2:4018622/1‑76 (MQ=255)
tGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCaa < 1:3754394/76‑1 (MQ=255)
tATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAAcc > 2:158293/1‑76 (MQ=255)
tATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAAcc > 2:3063547/1‑76 (MQ=255)
aTCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCa > 2:3906376/1‑76 (MQ=255)
ccGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGa > 1:4626635/1‑76 (MQ=255)
ccGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGa > 2:2612821/1‑76 (MQ=255)
cGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGAt < 2:577332/76‑1 (MQ=255)
gggTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATg < 1:1815886/76‑1 (MQ=255)
gAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCgt < 2:580661/76‑1 (MQ=255)
gAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCgt > 2:832763/1‑76 (MQ=255)
gAAGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCgt > 2:3231429/1‑76 (MQ=255)
aaGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCgtg > 1:954873/1‑76 (MQ=255)
aaGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCgtg > 1:2049941/1‑76 (MQ=255)
aGCAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCgtgt > 1:2749585/1‑76 (MQ=255)
cAGGTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCgtgtgt > 1:1551715/1‑76 (MQ=255)
gTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGtc < 1:1155331/76‑1 (MQ=255)
gTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGtc < 1:4282160/76‑1 (MQ=255)
gTATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGtc > 1:3466290/1‑76 (MQ=255)
tATCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGtct > 1:1032408/1‑76 (MQ=255)
aTCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACGCCTGTATCAACCAGATGCCGTGTGTGtctc > 1:767166/1‑76 (MQ=255)
aTCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGtctc < 1:2595159/76‑1 (MQ=255)
aTCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGtctc > 1:4954978/1‑76 (MQ=255)
tCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTctct < 1:4161250/76‑1 (MQ=255)
tCGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTctct > 2:4858518/1‑76 (MQ=255)
cGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTg > 1:2584087/1‑76 (MQ=255)
cGACATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTg < 1:2689516/76‑1 (MQ=255)
cATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGt > 1:240138/1‑76 (MQ=255)
cATCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGt < 1:4275282/76‑1 (MQ=255)
aTCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTg > 2:2042861/1‑76 (MQ=255)
aTCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTg > 2:4042384/1‑76 (MQ=255)
aTCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTg > 1:3931051/1‑76 (MQ=255)
tCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGa > 1:2806126/1‑76 (MQ=255)
tCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGa < 1:124433/76‑1 (MQ=255)
tCTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGa < 1:2052610/76‑1 (MQ=255)
cTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGaa < 1:2207921/76‑1 (MQ=255)
cTACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGaa > 2:4632654/1‑76 (MQ=255)
tACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAAc > 2:1702084/1‑76 (MQ=255)
tACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAAc > 2:3152066/1‑76 (MQ=255)
tACAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAAc > 2:1444030/1‑76 (MQ=255)
aCAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAAcc > 1:1348498/1‑76 (MQ=255)
cAACCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCg > 2:3870223/1‑76 (MQ=255)
cAACCGGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCg > 2:964501/1‑76 (MQ=255)
aCCTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGt > 1:4378763/1‑76 (MQ=255)
cTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTg < 2:3828975/76‑1 (MQ=255)
cTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTg > 2:845176/1‑76 (MQ=255)
cTGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTg < 1:1922930/76‑1 (MQ=255)
tGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGa > 2:2159441/1‑76 (MQ=255)
tGACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGa > 2:4452912/1‑76 (MQ=255)
gACCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGaa > 1:84334/1‑76 (MQ=255)
aCCAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAAc < 2:4228758/76‑1 (MQ=255)
ccAAATACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACg < 1:4785579/76‑1 (MQ=255)
aaaTACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTg > 2:223169/1‑76 (MQ=255)
aaaTACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTg > 1:967073/1‑76 (MQ=255)
aaTACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTgg < 2:1685083/76‑1 (MQ=255)
aaTACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTgg > 1:1505143/1‑76 (MQ=255)
aTACACCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGc > 1:3486694/1‑76 (MQ=255)
cacCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGAc < 1:1441213/76‑1 (MQ=255)
cacCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGAc > 1:2347957/1‑76 (MQ=255)
cacCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGAc > 2:3946946/1‑76 (MQ=255)
acCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACg < 1:4566169/76‑1 (MQ=255)
acCTGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACg > 2:2425573/1‑76 (MQ=255)
|
TATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACG > REL606/4163054‑4163199
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
