Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A120 F1 I1 R1
|
20 |
248.8 |
16334864 |
93.0% |
15191423 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,163,133 |
A→C |
N760H (AAC→CAC) |
rpoB → |
DNA‑directed RNA polymerase subunit beta |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,163,133 | 0 | A | C | 97.9%
| 1005.6
/ 4.6
| 233 | N760H (AAC→CAC) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base A (4/1); new base C (120/108); total (124/109) |
| Fisher's exact test for biased strand distribution p-value = 3.75e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 |
Only 100 of 236 total aligned reads displayed.
AGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGC > REL606/4163060‑4163206
|
aGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCAc < 2:846561/76‑1 (MQ=255)
aGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCAc > 2:3817007/1‑76 (MQ=255)
gTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCAcc < 2:765796/76‑1 (MQ=255)
ttAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCa > 1:2500829/1‑76 (MQ=255)
ttAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCa > 1:546794/1‑76 (MQ=255)
ttAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCa > 1:6781587/1‑76 (MQ=255)
ttAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCa > 1:2069650/1‑76 (MQ=255)
ttAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCa > 1:1291617/1‑76 (MQ=255)
tAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAg > 1:163911/1‑76 (MQ=255)
tAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAg < 1:3058280/76‑1 (MQ=255)
tAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAg > 1:7122658/1‑76 (MQ=255)
aaCGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGa > 2:2034417/1‑76 (MQ=255)
aCGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGaa > 1:800569/1‑76 (MQ=255)
cGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAac < 2:2442297/76‑1 (MQ=255)
gAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAaca < 2:1619737/76‑1 (MQ=255)
gAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAaca < 1:6867458/76‑1 (MQ=255)
gAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAaca > 1:4456644/1‑76 (MQ=255)
aaGACGCGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAacac < 2:6026984/76‑1 (MQ=255)
aCGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTg < 1:7815189/76‑1 (MQ=255)
aCGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTg > 2:1185261/1‑76 (MQ=255)
aCGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTg > 1:7081074/1‑76 (MQ=255)
cGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGt > 2:1662034/1‑76 (MQ=255)
cGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGt < 2:7041310/76‑1 (MQ=255)
agaTGTATGCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTAt < 2:6380826/76‑1 (MQ=255)
agaTGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTAt < 1:1608994/76‑1 (MQ=255)
gaTGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATc > 2:432894/1‑76 (MQ=255)
gaTGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATc < 2:7740437/76‑1 (MQ=255)
aTGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCa < 1:4609138/76‑1 (MQ=255)
aTGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCa < 1:5177133/76‑1 (MQ=255)
gTATCCGGGTGAAGCAGGTATCGACATCTCCAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAAc > 1:6646533/1‑76 (MQ=255)
gTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAAc > 1:4020329/1‑76 (MQ=255)
gTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAAc > 1:6747219/1‑76 (MQ=255)
gTACCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAAc < 1:4931140/76‑1 (MQ=255)
tATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAAcc > 1:6543574/1‑76 (MQ=255)
aTCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCa < 1:2431667/76‑1 (MQ=255)
aTCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCa < 1:4390161/76‑1 (MQ=255)
tCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAg < 2:823940/76‑1 (MQ=255)
ccGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGa < 1:3217141/76‑1 (MQ=255)
ccGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGa < 1:5903386/76‑1 (MQ=255)
cGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGAt > 2:684148/1‑76 (MQ=255)
tgTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGc > 2:2857039/2‑76 (MQ=255)
tGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCg < 1:1132418/76‑1 (MQ=255)
gAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCgt < 2:3752440/76‑1 (MQ=255)
aaGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCgtg < 2:1510034/76‑1 (MQ=255)
cAGGTATCGACATCTACAACCTGACCAAATACTCCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCgtgtgt < 1:4740676/76‑1 (MQ=255)
cAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCgtgtgt > 1:7953550/1‑76 (MQ=255)
ggTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCgtgtgtgt < 2:6141784/76‑1 (MQ=255)
ggTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCgtgtgtgt > 1:6095155/1‑76 (MQ=255)
ggTATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCgtgtgtgt > 1:5300951/1‑76 (MQ=255)
tATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGtct < 2:5104767/76‑1 (MQ=255)
tATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGtct > 1:4544830/1‑76 (MQ=255)
tATCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGtct < 2:6592290/76‑1 (MQ=255)
aTCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGtctc > 1:1839237/1‑76 (MQ=255)
aTCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGtctc > 2:3279998/1‑76 (MQ=255)
tCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTctct > 1:7292852/1‑76 (MQ=255)
tCGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTctct > 2:5339621/1‑76 (MQ=255)
cGACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTg > 1:3512946/1‑76 (MQ=255)
gACATCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTgg > 1:813202/1‑76 (MQ=255)
cATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGt > 2:263378/1‑76 (MQ=255)
aTCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTg > 1:4160290/1‑76 (MQ=255)
aTCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTg > 2:5032134/1‑76 (MQ=255)
tCTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGa < 1:7220638/76‑1 (MQ=255)
cTACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGaa > 2:4521220/1‑76 (MQ=255)
tACAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAAc < 2:5260971/76‑1 (MQ=255)
aCAACCTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAAcc < 1:2809483/76‑1 (MQ=255)
ccTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCGGATGCCGTGTGTGTCTCTGGGTGAACCGGtt < 1:3299544/76‑1 (MQ=255)
ccTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGtt < 1:2161034/76‑1 (MQ=255)
ccTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGtt > 1:3011195/1‑76 (MQ=255)
cTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTg > 2:6726771/1‑76 (MQ=255)
cTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTg < 2:2917643/76‑1 (MQ=255)
cTGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTCTGTGTCTCTGGGTGAACCGGTTg > 1:5970374/1‑76 (MQ=255)
tGACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGa > 2:3530902/1‑76 (MQ=255)
gACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGaa > 1:6314234/1‑76 (MQ=255)
gACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGaa < 2:4871750/76‑1 (MQ=255)
gACCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGCGAACCGGTTGaa > 2:6258502/1‑76 (MQ=255)
aCCAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAAc < 2:4412043/76‑1 (MQ=255)
ccAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACg < 2:6747219/76‑1 (MQ=255)
cAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGt > 1:6454560/1‑76 (MQ=255)
cAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGt < 1:1505361/76‑1 (MQ=255)
cAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGt > 1:2624902/1‑76 (MQ=255)
cAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGt > 1:7392323/1‑76 (MQ=255)
cAAATACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGt > 1:1011705/1‑76 (MQ=255)
aaaTACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTg > 1:5724443/1‑76 (MQ=255)
aaaTACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTg > 1:3568647/1‑76 (MQ=255)
aTACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGc < 2:1447300/76‑1 (MQ=255)
aTACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGc > 2:2115051/1‑76 (MQ=255)
tACACCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCg > 2:3478392/1‑76 (MQ=255)
acacCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGa > 1:3412250/1‑76 (MQ=255)
acacCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGa > 1:4825841/1‑76 (MQ=255)
acacCCGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGa > 2:7074268/1‑76 (MQ=255)
cccGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGt < 2:7426123/76‑1 (MQ=255)
ccGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTg > 2:3165583/1‑76 (MQ=255)
ccGTTCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTg > 2:3465536/1‑76 (MQ=255)
ttCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTGTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTg > 2:5600816/1‑76 (MQ=255)
ttCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTg < 1:3765954/76‑1 (MQ=255)
ttCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTg > 2:2087345/1‑76 (MQ=255)
ttCTCACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTg < 2:291541/76‑1 (MQ=255)
tctcACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTgg > 1:4983733/1‑76 (MQ=255)
tctcACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTgg > 2:3013237/1‑76 (MQ=255)
cTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGc > 2:5867507/1‑76 (MQ=255)
|
AGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGCCGTGTGTGTCTCTGGGTGAACCGGTTGAACGTGGCGACGTGCTGGC > REL606/4163060‑4163206
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
