breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 649,731 C→A W357C (TGG→TGT mrdA ← transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)
RA 762,305 C→G intergenic (+77/‑56) lysZ → / → lysQ tRNA‑Lys/tRNA‑Lys
RA 1,329,516 C→T H33Y (CAC→TAC)  topA → DNA topoisomerase I
RA 1,332,157 A→G intergenic (+140/‑70) topA → / → cysB DNA topoisomerase I/DNA‑binding transcriptional dual regulator, O‑acetyl‑L‑serine‑binding
JC 1,733,297 (CGTATGAA)1→2 coding (333/1413 nt) pykF → pyruvate kinase
JC JC 1,988,481 IS150 (–) +3 bp coding (5620‑5622/7152 nt) yeeJ → adhesin
RA 2,131,466 (A)9→8 intergenic (‑125/+175) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase
RA 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein
JC JC 2,448,488 IS4 (+) +14 bp intergenic (+19/+18) nupC → / ← yfeA nucleoside (except guanosine) transporter/predicted diguanylate cyclase
JC JC 3,015,254 IS150 (+) +3 bp coding (675‑677/1677 nt) ECB_02816 → KpsD protein
JC JC 3,103,274 IS150 (–) +3 bp coding (128‑130/483 nt) ygiV ← predicted transcriptional regulator
JC 3,147,777 Δ3 bp intergenic (+72/+5) rpoD → / ← ygjF RNA polymerase sigma factor/G/U mismatch‑specific DNA glycosylase
MC JC 3,228,601 Δ557 bp [yraM] [yraM]
RA 3,338,855 G→A G229R (GGA→AGA)  yhdG → tRNA‑dihydrouridine synthase B
RA 3,762,741 A→T K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,875,632 (T)7→8 intergenic (‑66/+287) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
MC JC 3,894,997 Δ6,934 bp IS150‑mediated rbsD[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
RA 3,911,972 (T)7→8 intergenic (‑173/‑150) yifB ← / → ilvL predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide
RA 4,163,133 A→C N760H (AAC→CAC)  rpoB → DNA‑directed RNA polymerase subunit beta
JC JC 4,524,522 IS186 (+) +6 bp coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)