breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 221 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL606720,4660GC63.2% ‑0.1 / 10.7 19A23P (GCC→CCC) phrBdeoxyribodipyrimidine photolyase, FAD‑binding
*REL606720,4640CG55.2% ‑0.1 / 38.4 29A22G (GCC→GGC) phrBdeoxyribodipyrimidine photolyase, FAD‑binding
*REL6062,515,9090AC53.6% 41.1 / 95.9 70V260G (GTG→GGG) ypfIpredicted hydrolase
*REL6062,775,1250AC53.3% ‑6.0 / 11.7 15V63G (GTA→GGA) insK‑3IS150 putative transposase
*REL6061,747,9030GT44.4% 428.3 / inf 487A8S (GCA→TCA) ydiLhypothetical protein
*REL6064,180,2490TG43.0% 167.4 / 44.9 114G24G (GGT→GGGyjaHhypothetical protein
*REL6064,392,5560CT41.2% 162.2 / 121.6 160L43L (CTA→TTA) aidBisovaleryl CoA dehydrogenase
*REL6064,111,4290TG40.8% 107.1 / 12.2 71G496G (GGT→GGGmetLbifunctional aspartate kinase II/homoserine dehydrogenase II
*REL6061,854,4010AC40.0% 101.9 / 30.2 80K88N (AAA→AACyeaOhypothetical protein
*REL6062,044,3570AC39.3% 334.2 / 133.2 211V284G (GTG→GGG) rfbDdTDP‑4‑dehydrorhamnose reductase subunit, NAD(P)‑binding, of dTDP‑L‑rhamnose synthase
*REL6064330TG38.3% 147.1 / 36.8 95V33G (GTG→GGG) thrAbifunctional aspartokinase I/homeserine dehydrogenase I
*REL6062,144,2950AC38.2% 388.8 / 120.4 228G357G (GGT→GGGmrpantiporter inner membrane protein
*REL6063,774,9140CG37.1% 215.6 / 113.0 140A20P (GCC→CCC) yicJpredicted transporter
*REL6062,437,4060GT36.7% 42.5 / 14.6 30D435E (GAC→GAAypdDfused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component
*REL6064,169,2250TG36.6% 408.0 / 21.8 205intergenic (+41/‑172)rpoC/htrCDNA‑directed RNA polymerase subunit beta'/heat shock protein
*REL6061,853,0670AC36.0% 238.5 / 93.4 150T56P (ACC→CCC) yeaNpredicted transporter
*REL6062,055,8080CG35.5% 135.6 / 46.0 76A97P (GCC→CCC) cpsGphosphomannomutase
*REL6061,118,2090GT35.5% 64.3 / 49.4 62intergenic (‑444/‑311)csgD/csgBDNA‑binding transcriptional activator in two‑component regulatory system/curlin nucleator protein, minor subunit in curli complex
*REL6062,356,0140TG35.2% 329.2 / 49.5 177T58P (ACC→CCC) yfbUhypothetical protein
*REL6063,217,4340GC35.0% 36.6 / 15.1 40A76P (GCC→CCC) agaStagatose‑6‑phosphate ketose/aldose isomerase

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 2042218 =293 (1.200)5 (0.020) 4/134 17.4 1.9% coding (603/1428 nt) wzx O antigen translocase (Wzx)
?REL606 2042455 = 257 (1.180)coding (366/1428 nt) wzx O antigen translocase (Wzx)
* ? REL606 = 2583282527 (2.150)118 (0.480) 5/150 17.6 22.7% coding (741/741 nt) yfhQ predicted methyltransferase
?REL606 3319969 = 275 (1.120)intergenic (‑50/+380) tldD/yhdP predicted peptidase/conserved membrane protein, predicted transporter
* ? REL606 796221 =211 (0.860)3 (0.010) 3/128 17.8 1.4% coding (109/993 nt) ECB_00737 hypothetical protein
?REL606 796406 = 235 (1.130)intergenic (‑77/‑349) ECB_00737/ECB_00738 hypothetical protein/hypothetical protein
* ? REL606 2583007 =542 (2.210)117 (0.500) 4/144 18.0 18.5% intergenic (‑177/+275) iscR/yfhQ DNA‑binding transcriptional repressor/predicted methyltransferase
?REL606 = 2583279 511 (2.170)intergenic (‑449/+3) iscR/yfhQ DNA‑binding transcriptional repressor/predicted methyltransferase
* ? REL606 3850562 =343 (1.400)4 (0.020) 3/134 18.2 1.3% intergenic (+56/‑35) tnaA/tnaB tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent/tryptophan transporter of low affinity
?REL606 3850779 = 296 (1.350)coding (183/1248 nt) tnaB tryptophan transporter of low affinity
* ? REL606 2916695 =277 (1.130)4 (0.020) 3/136 18.3 1.5% intergenic (‑10/‑426) ygfT/ygfU fused predicted oxidoreductase: Fe‑S subunit/nucleotide‑binding subunit/predicted transporter
?REL606 2916914 = 287 (1.290)intergenic (‑229/‑207) ygfT/ygfU fused predicted oxidoreductase: Fe‑S subunit/nucleotide‑binding subunit/predicted transporter
* ? REL606 = 20123317 (1.290)4 (0.020) 3/138 18.4 1.5% coding (495/960 nt) ECB_00020 hypothetical protein
?REL606 = 20373 242 (1.070)coding (245/960 nt) ECB_00020 hypothetical protein
* ? REL606 = 4603902455 (1.860)9 (0.040) 3/146 18.8 2.4% intergenic (+408/+12) yjjV/yjjW predicted DNase/predicted pyruvate formate lyase activating enzyme
?REL606 = 4621379 289 (1.210)intergenic (+80/+5) trpR/yjjX Trp operon repressor/NTPase