breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 221 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | REL606 | 720,466 | 0 | G | C | 63.2% | ‑0.1 / 10.7 | 19 | A23P (GCC→CCC) | phrB | deoxyribodipyrimidine photolyase, FAD‑binding |
* | REL606 | 720,464 | 0 | C | G | 55.2% | ‑0.1 / 38.4 | 29 | A22G (GCC→GGC) | phrB | deoxyribodipyrimidine photolyase, FAD‑binding |
* | REL606 | 2,515,909 | 0 | A | C | 53.6% | 41.1 / 95.9 | 70 | V260G (GTG→GGG) | ypfI | predicted hydrolase |
* | REL606 | 2,775,125 | 0 | A | C | 53.3% | ‑6.0 / 11.7 | 15 | V63G (GTA→GGA) | insK‑3 | IS150 putative transposase |
* | REL606 | 1,747,903 | 0 | G | T | 44.4% | 428.3 / inf | 487 | A8S (GCA→TCA) | ydiL | hypothetical protein |
* | REL606 | 4,180,249 | 0 | T | G | 43.0% | 167.4 / 44.9 | 114 | G24G (GGT→GGG) | yjaH | hypothetical protein |
* | REL606 | 4,392,556 | 0 | C | T | 41.2% | 162.2 / 121.6 | 160 | L43L (CTA→TTA) | aidB | isovaleryl CoA dehydrogenase |
* | REL606 | 4,111,429 | 0 | T | G | 40.8% | 107.1 / 12.2 | 71 | G496G (GGT→GGG) | metL | bifunctional aspartate kinase II/homoserine dehydrogenase II |
* | REL606 | 1,854,401 | 0 | A | C | 40.0% | 101.9 / 30.2 | 80 | K88N (AAA→AAC) | yeaO | hypothetical protein |
* | REL606 | 2,044,357 | 0 | A | C | 39.3% | 334.2 / 133.2 | 211 | V284G (GTG→GGG) | rfbD | dTDP‑4‑dehydrorhamnose reductase subunit, NAD(P)‑binding, of dTDP‑L‑rhamnose synthase |
* | REL606 | 433 | 0 | T | G | 38.3% | 147.1 / 36.8 | 95 | V33G (GTG→GGG) | thrA | bifunctional aspartokinase I/homeserine dehydrogenase I |
* | REL606 | 2,144,295 | 0 | A | C | 38.2% | 388.8 / 120.4 | 228 | G357G (GGT→GGG) | mrp | antiporter inner membrane protein |
* | REL606 | 3,774,914 | 0 | C | G | 37.1% | 215.6 / 113.0 | 140 | A20P (GCC→CCC) | yicJ | predicted transporter |
* | REL606 | 2,437,406 | 0 | G | T | 36.7% | 42.5 / 14.6 | 30 | D435E (GAC→GAA) | ypdD | fused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component |
* | REL606 | 4,169,225 | 0 | T | G | 36.6% | 408.0 / 21.8 | 205 | intergenic (+41/‑172) | rpoC/htrC | DNA‑directed RNA polymerase subunit beta'/heat shock protein |
* | REL606 | 1,853,067 | 0 | A | C | 36.0% | 238.5 / 93.4 | 150 | T56P (ACC→CCC) | yeaN | predicted transporter |
* | REL606 | 2,055,808 | 0 | C | G | 35.5% | 135.6 / 46.0 | 76 | A97P (GCC→CCC) | cpsG | phosphomannomutase |
* | REL606 | 1,118,209 | 0 | G | T | 35.5% | 64.3 / 49.4 | 62 | intergenic (‑444/‑311) | csgD/csgB | DNA‑binding transcriptional activator in two‑component regulatory system/curlin nucleator protein, minor subunit in curli complex |
* | REL606 | 2,356,014 | 0 | T | G | 35.2% | 329.2 / 49.5 | 177 | T58P (ACC→CCC) | yfbU | hypothetical protein |
* | REL606 | 3,217,434 | 0 | G | C | 35.0% | 36.6 / 15.1 | 40 | A76P (GCC→CCC) | agaS | tagatose‑6‑phosphate ketose/aldose isomerase |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | 2042218 = | 293 (1.200) | 5 (0.020) | 4/134 | 17.4 | 1.9% | coding (603/1428 nt) | wzx | O antigen translocase (Wzx) |
? | REL606 | 2042455 = | 257 (1.180) | coding (366/1428 nt) | wzx | O antigen translocase (Wzx) | |||||
* | ? | REL606 | = 2583282 | 527 (2.150) | 118 (0.480) | 5/150 | 17.6 | 22.7% | coding (741/741 nt) | yfhQ | predicted methyltransferase |
? | REL606 | 3319969 = | 275 (1.120) | intergenic (‑50/+380) | tldD/yhdP | predicted peptidase/conserved membrane protein, predicted transporter | |||||
* | ? | REL606 | 796221 = | 211 (0.860) | 3 (0.010) | 3/128 | 17.8 | 1.4% | coding (109/993 nt) | ECB_00737 | hypothetical protein |
? | REL606 | 796406 = | 235 (1.130) | intergenic (‑77/‑349) | ECB_00737/ECB_00738 | hypothetical protein/hypothetical protein | |||||
* | ? | REL606 | 2583007 = | 542 (2.210) | 117 (0.500) | 4/144 | 18.0 | 18.5% | intergenic (‑177/+275) | iscR/yfhQ | DNA‑binding transcriptional repressor/predicted methyltransferase |
? | REL606 | = 2583279 | 511 (2.170) | intergenic (‑449/+3) | iscR/yfhQ | DNA‑binding transcriptional repressor/predicted methyltransferase | |||||
* | ? | REL606 | 3850562 = | 343 (1.400) | 4 (0.020) | 3/134 | 18.2 | 1.3% | intergenic (+56/‑35) | tnaA/tnaB | tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent/tryptophan transporter of low affinity |
? | REL606 | 3850779 = | 296 (1.350) | coding (183/1248 nt) | tnaB | tryptophan transporter of low affinity | |||||
* | ? | REL606 | 2916695 = | 277 (1.130) | 4 (0.020) | 3/136 | 18.3 | 1.5% | intergenic (‑10/‑426) | ygfT/ygfU | fused predicted oxidoreductase: Fe‑S subunit/nucleotide‑binding subunit/predicted transporter |
? | REL606 | 2916914 = | 287 (1.290) | intergenic (‑229/‑207) | ygfT/ygfU | fused predicted oxidoreductase: Fe‑S subunit/nucleotide‑binding subunit/predicted transporter | |||||
* | ? | REL606 | = 20123 | 317 (1.290) | 4 (0.020) | 3/138 | 18.4 | 1.5% | coding (495/960 nt) | ECB_00020 | hypothetical protein |
? | REL606 | = 20373 | 242 (1.070) | coding (245/960 nt) | ECB_00020 | hypothetical protein | |||||
* | ? | REL606 | = 4603902 | 455 (1.860) | 9 (0.040) | 3/146 | 18.8 | 2.4% | intergenic (+408/+12) | yjjV/yjjW | predicted DNase/predicted pyruvate formate lyase activating enzyme |
? | REL606 | = 4621379 | 289 (1.210) | intergenic (+80/+5) | trpR/yjjX | Trp operon repressor/NTPase |