Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A66 F1 I1 R1
|
19 |
57.4 |
3651244 |
97.1% |
3545357 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,928,833 |
T→C |
V206A (GTT→GCT) |
rho → |
transcription termination factor Rho |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,928,833 | 0 | T | C | 98.0%
| 193.5
/ ‑4.1
| 49 | V206A (GTT→GCT) | rho | transcription termination factor Rho |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (29/19); total (30/19) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01 |
AAGCCGGTAAAACCATGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTC > REL606/3928758‑3928905
|
aaGCCGGTAAAACCATGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGt > 2:1686747/1‑76 (MQ=255)
ccGGTAAAACCATGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGctct > 1:46537/1‑76 (MQ=255)
gTAAAACCATGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTctgct < 1:596285/76‑1 (MQ=255)
gTAAAACCATGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTctgct < 1:596297/76‑1 (MQ=255)
tAAAACCATGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTctgctg > 1:632138/1‑76 (MQ=255)
aaaaCCATGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGa > 1:913870/1‑76 (MQ=255)
aaCCATGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATc > 1:651202/1‑76 (MQ=255)
aCCATGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATcg < 1:1441294/76‑1 (MQ=255)
aTGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATcgacg < 1:1735032/76‑1 (MQ=255)
aTGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATcgacg > 2:1430147/1‑76 (MQ=255)
gctgctgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGaa < 1:1500281/76‑1 (MQ=255)
gctgctgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGaa < 2:1693156/76‑1 (MQ=255)
gctgctgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGaa < 1:279205/76‑1 (MQ=255)
ctgctgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAAc > 1:1413205/1‑76 (MQ=255)
ctgctgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGAGTGTGTGCTGATGGCTCTGCTGATCGACGAAc < 1:1476436/76‑1 (MQ=255)
tgctgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACg > 2:1528170/1‑76 (MQ=255)
gctgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGt > 2:1802546/1‑76 (MQ=255)
ctgcAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTc > 2:633475/1‑76 (MQ=255)
cAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCgg > 2:441691/1‑76 (MQ=255)
aaCATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGgaag > 2:1698610/1‑76 (MQ=255)
aaCATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGgaag < 2:562922/76‑1 (MQ=255)
tGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTa < 2:1413340/76‑1 (MQ=255)
gCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTaa > 1:685072/1‑76 (MQ=255)
cAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCg > 1:808938/1‑76 (MQ=255)
agagCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCga > 1:1621264/1‑76 (MQ=255)
gagCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCgag < 2:318107/76‑1 (MQ=255)
gagCATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCgag > 2:1158489/1‑76 (MQ=255)
cATTGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATg < 2:1821536/76‑1 (MQ=255)
ttGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCa > 1:1825252/1‑76 (MQ=255)
ttGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCa > 1:1734511/1‑76 (MQ=255)
tGCTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAg > 1:897780/1‑76 (MQ=255)
cTTACAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCg > 1:678736/1‑76 (MQ=255)
aCAACCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCt < 1:1131924/76‑1 (MQ=255)
aaCCACCCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTgg < 1:1078129/76‑1 (MQ=255)
accaccCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGt < 1:609709/76‑1 (MQ=255)
accaccCAGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGt < 1:609708/76‑1 (MQ=255)
caccCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTaa < 1:684341/76‑1 (MQ=255)
caccCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTaa > 1:515705/1‑76 (MQ=255)
accCGGATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGGaaa > 2:1012464/1‑76 (MQ=255)
cgtaTTGTGTGCTGATGACTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAgg < 2:431916/73‑1 (MQ=255)
ggATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGt < 2:1471344/76‑1 (MQ=255)
ggATTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGt < 1:136073/76‑1 (MQ=255)
aTTGTGTGCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGa > 2:853267/1‑76 (MQ=255)
tgtgCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAgtt > 1:446922/1‑76 (MQ=255)
gtgCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAgttg > 1:1406841/1‑76 (MQ=255)
tgCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAgttgt > 2:978070/1‑76 (MQ=255)
tgCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAgttgt > 2:1723289/1‑76 (MQ=255)
gCTGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAgttgtt > 2:463379/1‑76 (MQ=255)
cTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGGGAAGttgttg > 1:386551/1‑76 (MQ=255)
tGATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGc > 2:90718/1‑76 (MQ=255)
gATGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCt > 1:149894/1‑76 (MQ=255)
tGGCTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTc > 2:1244189/1‑76 (MQ=255)
|
AAGCCGGTAAAACCATGCTGCTGCAGAACATTGCTCAGAGCATTGCTTACAACCACCCGGATTGTGTGCTGATGGTTCTGCTGATCGACGAACGTCCGGAAGAAGTAACCGAGATGCAGCGTCTGGTAAAAGGTGAAGTTGTTGCTTC > REL606/3928758‑3928905
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
