Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A68 F1 I1 R1
|
17 |
56.4 |
3914180 |
96.2% |
3765441 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,881,536 |
T→G |
D7A (GAT→GCT) |
atpE ← |
F0F1 ATP synthase subunit C |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,881,536 | 0 | T | G | 94.9%
| 224.4
/ 0.9
| 59 | D7A (GAT→GCT) | atpE | F0F1 ATP synthase subunit C |
| Reads supporting (aligned to +/- strand): ref base T (3/0); new base G (26/30); total (29/30) |
| Fisher's exact test for biased strand distribution p-value = 1.12e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01 |
GAGGATGCCGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGATCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAAT > REL606/3881463‑3881602
|
gAGGATGCCGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGcc > 1:96831/1‑76 (MQ=255)
aGGATGCCGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCa > 1:1849434/1‑76 (MQ=255)
aGGATGCCGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCa < 1:850287/76‑1 (MQ=255)
aGGATGCCGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCa < 1:554595/76‑1 (MQ=255)
ggATGCCGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCat < 1:1681209/76‑1 (MQ=255)
aTGCCGATCCCGATCGCAGCCCCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCatat < 2:879887/76‑1 (MQ=255)
aTGCCGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCatat < 2:563263/76‑1 (MQ=255)
tGCCGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATAtt < 2:1502345/76‑1 (MQ=255)
tGCCGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATAtt > 2:674034/1‑76 (MQ=255)
ccGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCa > 2:1342449/1‑76 (MQ=255)
aTACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGt > 2:1727231/1‑76 (MQ=255)
aTACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGt > 2:543373/1‑76 (MQ=255)
tACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGtt < 1:1075733/76‑1 (MQ=255)
aCCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGttt > 2:1662094/1‑76 (MQ=255)
aCCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGttt > 2:218404/1‑76 (MQ=255)
cGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTc < 2:181549/76‑1 (MQ=255)
gATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTcc < 1:1542313/76‑1 (MQ=255)
gATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTcc > 1:698669/1‑76 (MQ=255)
aTCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCa < 1:250867/76‑1 (MQ=255)
cGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATg > 2:1907446/1‑76 (MQ=255)
cagcaCCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGAc < 1:1612545/76‑1 (MQ=255)
cagcaCCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGAc > 2:1191093/1‑76 (MQ=255)
agcaCCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACa > 2:1560897/1‑76 (MQ=255)
agcaCCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACa > 2:555216/1‑76 (MQ=255)
gcaCCGATTGCCGCCAGGCCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAg < 2:759293/76‑1 (MQ=255)
gcaCCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAg < 2:414845/76‑1 (MQ=255)
gcaCCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAg < 2:759279/76‑1 (MQ=255)
aCCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGtc < 2:620383/76‑1 (MQ=255)
cGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGtctc > 2:78228/1‑76 (MQ=255)
gATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTcc < 1:283319/76‑1 (MQ=255)
aTTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCa < 2:491999/76‑1 (MQ=255)
ccgccAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGTCAGTCTCCCgttt > 2:612479/1‑76 (MQ=255)
ccgccAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAgttt < 2:723293/76‑1 (MQ=255)
aGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTc > 1:1936343/1‑76 (MQ=255)
aCCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAg > 2:108321/1‑76 (MQ=255)
aCCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAg < 1:13739/76‑1 (MQ=255)
atcatcACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTaa > 1:1721380/1‑76 (MQ=255)
atcatcACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTaa > 1:562144/1‑76 (MQ=255)
tcatcaCAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTaaa < 1:489541/76‑1 (MQ=255)
catcaCAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTaaaa < 2:1210526/76‑1 (MQ=255)
atcaCAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAAc < 2:146434/76‑1 (MQ=255)
cacaGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACgt < 2:1176293/76‑1 (MQ=255)
aGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACgtagt > 2:1509171/1‑76 (MQ=255)
aGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACgtagt < 1:978496/76‑1 (MQ=255)
aGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACgtagt > 1:29919/1‑76 (MQ=255)
aGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACgtagt < 1:218404/76‑1 (MQ=255)
ggCAGCCATGTACAGCAGATCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTagtagt > 1:735179/1‑76 (MQ=255)
ggCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTagtagt > 1:1269274/1‑76 (MQ=255)
gCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAgtg > 1:1036839/1‑76 (MQ=255)
cAGCCATGTACAGCAGATCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAgtgt > 1:1611440/1‑76 (MQ=255)
cAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAgtgt > 2:825187/1‑76 (MQ=255)
aGCCATGTACAGCAGATCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGtt > 1:1574031/1‑76 (MQ=255)
aGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGtt < 2:1211940/76‑1 (MQ=255)
cATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGt < 2:218380/76‑1 (MQ=255)
aTGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTa < 2:1919488/76‑1 (MQ=255)
aTGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTa > 1:828433/1‑76 (MQ=255)
gTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTaaa < 2:135568/76‑1 (MQ=255)
gTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTaaa < 2:90613/76‑1 (MQ=255)
tACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAAt > 1:442730/1‑76 (MQ=255)
|
GAGGATGCCGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGATCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAAT > REL606/3881463‑3881602
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
