Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A80 F1 I1 R1
|
17 |
48.4 |
3161007 |
95.9% |
3031405 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,359,374 |
T→C |
F346L (TTC→CTC) |
pta → |
phosphate acetyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,359,374 | 0 | T | C | 100.0%
| 174.8
/ NA
| 42 | F346L (TTC→CTC) | pta | phosphate acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (26/16); total (26/16) |
TGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACAT > REL606/2359301‑2359444
|
tGCTTTCGCTCCCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTc < 1:616718/76‑1 (MQ=255)
tGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTc < 1:1310245/76‑1 (MQ=255)
gCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCa > 1:1047719/1‑76 (MQ=255)
gCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCa < 2:1347402/76‑1 (MQ=255)
tttCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAAc < 1:1012880/76‑1 (MQ=255)
cGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTg > 1:1312393/1‑76 (MQ=255)
cGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTg > 1:683322/1‑76 (MQ=255)
cTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGa > 2:1007895/1‑76 (MQ=255)
cTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGa > 1:798068/1‑76 (MQ=255)
cTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGa > 1:1520265/1‑76 (MQ=255)
tACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGaa > 2:732400/1‑76 (MQ=255)
aCCGGCCTGCCGGTATTTATGGTGAACACCAACACCTCGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAg > 2:52524/1‑76 (MQ=255)
cGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGtt < 2:1338019/76‑1 (MQ=255)
gCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTcc > 1:1428865/1‑76 (MQ=255)
gCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGtt > 1:33186/1‑76 (MQ=255)
aTTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGAt > 1:431709/1‑76 (MQ=255)
ttATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCa > 1:1273659/1‑76 (MQ=255)
ttATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCa > 1:1362294/1‑76 (MQ=255)
aTGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACg > 1:745759/1‑76 (MQ=255)
ggTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGaa > 1:896728/1‑76 (MQ=255)
gTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAAc < 1:1562627/76‑1 (MQ=255)
gTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAAc < 2:292967/76‑1 (MQ=255)
caacaccTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCgag > 2:1167460/1‑76 (MQ=255)
aacaccTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCgaga < 1:1482665/76‑1 (MQ=255)
aacaccTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCgaga > 2:173047/1‑76 (MQ=255)
ccTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGt < 1:487339/76‑1 (MQ=255)
cTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGtt < 1:815526/76‑1 (MQ=255)
cTGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGtt < 2:1375248/76‑1 (MQ=255)
tGGCAGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTc > 2:92279/1‑76 (MQ=255)
aGACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGa > 2:194689/1‑76 (MQ=255)
gACCTCTCTGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGaa < 1:1081712/76‑1 (MQ=255)
ctctGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGt > 1:1116720/1‑76 (MQ=255)
ctGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTg > 1:1473558/1‑76 (MQ=255)
ctGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTg > 1:104822/1‑76 (MQ=255)
tGAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGc < 2:44012/76‑1 (MQ=255)
gAGCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCt > 2:1372620/1‑76 (MQ=255)
gCCTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTaa < 1:12472/76‑1 (MQ=255)
ccTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAAc > 1:75001/1‑76 (MQ=255)
cTGCAGAGCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGCTCAGGAATACGTTGCTAACt > 1:1357576/1‑76 (MQ=255)
agagCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACAt < 2:126047/76‑1 (MQ=255)
agagCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACAt > 2:442619/1‑76 (MQ=255)
agagCCTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACAt < 2:828631/76‑1 (MQ=255)
|
TGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCTCTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACAT > REL606/2359301‑2359444
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
