Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I1 R1
|
20 |
51.0 |
3438118 |
91.8% |
3156192 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
1,400,364 |
T→C |
D21G (GAT→GGT) |
abgB ← |
predicted peptidase, aminobenzoyl‑glutamate utilization protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 1,400,364 | 0 | T | C | 98.0%
| 197.6
/ ‑3.9
| 50 | D21G (GAT→GGT) | abgB | predicted peptidase, aminobenzoyl‑glutamate utilization protein |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (23/26); total (24/26) |
| Fisher's exact test for biased strand distribution p-value = 4.80e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 |
CGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATATCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAGATTTCCTGCATCAGATACCTC > REL606/1400291‑1400435
|
cGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCg < 1:835655/76‑1 (MQ=255)
aGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGta > 2:431304/1‑76 (MQ=255)
cAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATaa > 2:619310/1‑76 (MQ=255)
cAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATaa < 1:638728/76‑1 (MQ=255)
ataTGCTCCGCTGACCACAACTCATGAAAACGTGTTTCTNGATGATCCCAGATTTNATCGGCAATACCGGTATAAc > 2:1693519/3‑76 (MQ=255)
gCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTg > 2:1200627/1‑76 (MQ=255)
ccGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCg < 2:912239/76‑1 (MQ=255)
ccGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCg < 2:818646/76‑1 (MQ=255)
tGACCAGAACTCTTCGAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCg < 1:1415187/76‑1 (MQ=255)
gACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCgg > 2:1280939/1‑76 (MQ=255)
gACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCgg > 2:733394/1‑76 (MQ=255)
gACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCgg > 2:383268/1‑76 (MQ=255)
aGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTc < 1:447651/76‑1 (MQ=255)
aGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTc < 2:617317/76‑1 (MQ=255)
aGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTc < 2:162293/76‑1 (MQ=255)
gAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCa < 1:617807/76‑1 (MQ=255)
ctctTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATc > 1:196758/1‑76 (MQ=255)
tctTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCg > 1:380517/1‑76 (MQ=255)
ctTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGc > 2:638284/1‑76 (MQ=255)
ttCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCa < 2:933851/76‑1 (MQ=255)
tCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCAt > 2:666215/1‑76 (MQ=255)
aaaCGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCAtcgt < 1:422511/76‑1 (MQ=255)
aaaCGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCAtcgt > 1:286747/1‑76 (MQ=255)
aaaCGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCAtcgt < 1:1297006/76‑1 (MQ=255)
aCGTGTTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCAtcgtcg < 2:646328/76‑1 (MQ=255)
gtTTCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATaa < 1:296464/76‑1 (MQ=255)
ttCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATaaaa < 1:765210/76‑1 (MQ=255)
ttCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATaaaa > 2:1143450/1‑76 (MQ=255)
ttCTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATaaaa > 1:1151050/1‑76 (MQ=255)
ntGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACg > 1:473979/2‑76 (MQ=255)
cTGGATGATCCCAGATTTGATCGGGAATACCGGTATAACGTTGGCGATCGGCTGTACTGGCGTCGTCGATaaaaag > 1:76753/1‑74 (MQ=255)
cTGGATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACg > 1:1354511/1‑76 (MQ=255)
ggATGATCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGAt < 1:579535/76‑1 (MQ=255)
gatgatCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATa < 1:1362448/76‑1 (MQ=255)
atgatCCCAGATTTGATCGGCAATATCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAg > 2:747042/1‑76 (MQ=255)
atgatCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAg < 1:1353303/76‑1 (MQ=255)
tgatCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAGa < 1:496756/76‑1 (MQ=255)
gatCCCAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAGAt > 1:1188898/1‑76 (MQ=255)
cccAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAGATTTc < 1:1143452/76‑1 (MQ=255)
cAGATTTGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAGATTTCCt < 1:1200629/76‑1 (MQ=255)
aTTTGATCGGCAATACCGGTATAACGTTGGCGATCAGCTTCAATCGCATCGTCGATAAAACGATAGATTTCCTGCa < 2:1370849/76‑1 (MQ=255)
tttGATCGGCAATACCGGCATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAGATTTCCTGCAt > 1:1003658/1‑76 (MQ=255)
ttGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAGATTTCCTGCATc < 2:1421959/76‑1 (MQ=255)
tGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAGATTTCNTGCATCa < 2:1375690/76‑1 (MQ=255)
tGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAGATTTCCTGCATCa < 1:1423155/76‑1 (MQ=255)
tGATCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAGATTTCCTGCATCa < 1:765274/76‑1 (MQ=255)
tCGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAGATTTCCTGCATCAGAt > 1:1205495/1‑76 (MQ=255)
cGGCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAGATTTCCTGCATCAGATa > 2:787513/1‑76 (MQ=255)
gCAATACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAGATTTCCTGCATCAGATAcc > 2:1314661/1‑76 (MQ=255)
aaTACCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAGATTTCCTGCATCAGATACCTc > 2:161582/1‑76 (MQ=255)
|
CGAAGCCAGATGCTCCGCTGACCAGAACTCTTCAAAACGTGTTTCTGGATGATCCCAGATTTGATCGGCAATATCGGTATAACGTTGGCGATCGGCTTCAATCGCATCGTCGATAAAACGATAGATTTCCTGCATCAGATACCTC > REL606/1400291‑1400435
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
