Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A120 F1 I1 R1
|
20 |
248.8 |
16334864 |
93.0% |
15191423 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,338,855 |
G→A |
G229R (GGA→AGA) |
yhdG → |
tRNA‑dihydrouridine synthase B |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,338,855 | 0 | G | A | 98.4%
| 955.2
/ 3.9
| 251 | G229R (GGA→AGA) | yhdG | tRNA‑dihydrouridine synthase B |
| Reads supporting (aligned to +/- strand): ref base G (3/1); new base A (129/117); total (133/118) |
| Fisher's exact test for biased strand distribution p-value = 6.24e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.04e-01 |
Only 100 of 276 total aligned reads displayed.
TGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGC > REL606/3338782‑3338928
|
tGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAgaga < 1:3126784/76‑1 (MQ=255)
gACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAACTCAGAGaa > 2:6694745/1‑76 (MQ=255)
aCCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAg < 1:3776757/76‑1 (MQ=255)
ccGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGAc < 2:8087837/76‑1 (MQ=255)
gCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGAccc > 2:4769971/1‑76 (MQ=255)
cTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCt > 2:42663/1‑76 (MQ=255)
cTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCt > 1:4663788/1‑76 (MQ=255)
ttAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTg < 2:435295/76‑1 (MQ=255)
ttAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTg > 1:2721138/1‑76 (MQ=255)
tAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTgg > 2:539410/1‑76 (MQ=255)
aaaGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGa > 2:3028160/1‑76 (MQ=255)
aaGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGAt < 2:7191742/76‑1 (MQ=255)
cAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCttt < 1:2464169/76‑1 (MQ=255)
cAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCttt < 1:904252/76‑1 (MQ=255)
cAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGACGCGCTGCTCAGAGAAGACCCTGGATCttt > 2:3684304/1‑76 (MQ=255)
agagCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTc < 1:2627006/76‑1 (MQ=255)
agagCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTc > 2:450158/1‑76 (MQ=255)
gagCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCg < 2:7435517/76‑1 (MQ=255)
agCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCgg < 1:1290743/76‑1 (MQ=255)
gCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCggg < 2:4285003/76‑1 (MQ=255)
tgtgCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGaa < 2:3615267/76‑1 (MQ=255)
tgtgCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGaa < 2:2439903/76‑1 (MQ=255)
tgtgCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGaa > 2:1341426/1‑76 (MQ=255)
gtgCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGGTCTTTCGGGaaa > 2:4668250/1‑76 (MQ=255)
gtgCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGaaa > 1:122353/1‑76 (MQ=255)
gtgCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGaaa > 2:2657149/1‑76 (MQ=255)
gtgCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTAGGGaaa > 1:3836039/1‑76 (MQ=255)
tgCTCGACTATACAGGGGCGGAGGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAAt > 2:1168588/1‑76 (MQ=255)
gCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATc > 1:1450315/1‑76 (MQ=255)
cTCGACTATACAGTGGCGGATGCCCTGATGATAGGCCGCGCATCTCAGAGAAGACCCTGGATCTTGCGGGAAATcc < 2:3759816/76‑1 (MQ=255)
cTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATcc < 2:3954710/76‑1 (MQ=255)
cTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATcc < 2:4999612/76‑1 (MQ=255)
cGACTATACAGGGGCGGATTCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAg > 1:8064215/1‑76 (MQ=255)
cGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAg > 1:4348329/1‑76 (MQ=255)
gACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGc > 2:6719567/1‑76 (MQ=255)
gACGATACAGGGGGGGGTGCCCTGCTGATAGGACGAGCGGCTGTCAGCACACCCAGCCGCTTTCGGGAAATccgca > 2:6746034/1‑73 (MQ=255)
aCTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCa > 1:5729815/1‑76 (MQ=255)
cTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCAt < 2:2569979/76‑1 (MQ=255)
tataCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCAtt > 1:2016928/1‑76 (MQ=255)
ataCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGGTCAGAGAAGAACCTGGATCTTTCGGGAAATACAGCATTa > 2:6579267/1‑76 (MQ=255)
ataCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCCGCATTa > 2:1617322/1‑76 (MQ=255)
ataCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTa < 1:438152/76‑1 (MQ=255)
ataCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTa > 1:7893144/1‑76 (MQ=255)
aCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATc < 1:5466129/76‑1 (MQ=255)
aCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATc < 1:4546695/76‑1 (MQ=255)
aCAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATc > 1:4962822/1‑76 (MQ=255)
cAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCt > 2:4833339/1‑76 (MQ=255)
aGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTg > 1:7055372/1‑76 (MQ=255)
gggCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGa < 1:5885565/76‑1 (MQ=255)
ggCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGac < 2:6984638/76‑1 (MQ=255)
ggCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGac < 2:4153746/76‑1 (MQ=255)
gCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGaca < 1:6531409/76‑1 (MQ=255)
gCGGATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGaca < 2:7500994/76‑1 (MQ=255)
ggATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACt > 1:6963304/1‑76 (MQ=255)
gATGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTg < 2:3252043/76‑1 (MQ=255)
aTGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTgg > 1:7996917/1‑76 (MQ=255)
aTGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTgg > 2:6200828/1‑76 (MQ=255)
aTGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTgg < 2:3865206/76‑1 (MQ=255)
tGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTggg > 1:2530551/1‑76 (MQ=255)
tGCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTggg < 1:7518339/76‑1 (MQ=255)
gCCCTGATGATAGGCCGCGCAGCTGAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTgggg < 2:2292187/76‑1 (MQ=255)
gCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACGCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTgggg > 1:1351787/1‑76 (MQ=255)
gCCCTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTgggg < 1:7805/76‑1 (MQ=255)
ccTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAg < 1:3277476/76‑1 (MQ=255)
ccTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAg < 1:1501235/76‑1 (MQ=255)
ccTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAg < 1:4783738/76‑1 (MQ=255)
ccTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAg > 1:8054489/1‑76 (MQ=255)
cTGATGATAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGt > 2:5089150/1‑76 (MQ=255)
tgatgaTAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGtt > 1:968486/1‑76 (MQ=255)
tgatgaTAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGtt > 2:581325/1‑76 (MQ=255)
tgatgaTAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGtt > 1:1094224/1‑76 (MQ=255)
tgatgaTAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGtt < 1:3644895/76‑1 (MQ=255)
gatgatAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTtg > 1:4082241/1‑76 (MQ=255)
gatgatAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTtg < 1:4824286/76‑1 (MQ=255)
gatgatAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTtg < 1:5263974/76‑1 (MQ=255)
gatgatAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTtg < 2:527175/76‑1 (MQ=255)
atgatAGGCCGCGCAGCTCAGAGAAGACGCTGGATCTTTCGGGAAATCCCGCATTATCTGGACACTGGGGAGTtgc > 2:360/1‑76 (MQ=255)
atgatAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTtgc < 1:724895/76‑1 (MQ=255)
tgatAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTtgct < 2:2759629/76‑1 (MQ=255)
gatAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTtgctg > 1:1874334/1‑76 (MQ=255)
tAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGcc > 1:6190330/1‑76 (MQ=255)
tAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGcc > 1:1751032/1‑76 (MQ=255)
tAGGCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGcc > 1:6828189/1‑76 (MQ=255)
ggCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGcccc > 1:2300140/1‑76 (MQ=255)
gCCGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGccccc < 1:5967152/76‑1 (MQ=255)
ccGCGCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCg < 1:7370775/76‑1 (MQ=255)
cgcgCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGc > 2:6342045/1‑76 (MQ=255)
cgcgCAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCACCGc > 2:1658660/1‑76 (MQ=255)
gcgcAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCt < 1:3076865/76‑1 (MQ=255)
gcAGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGc > 2:1383833/1‑76 (MQ=255)
aGCTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCt > 2:2909585/1‑76 (MQ=255)
cTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCttt > 1:6696296/1‑76 (MQ=255)
cTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCttt > 1:1000474/1‑76 (MQ=255)
cTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCttt > 2:4618429/1‑76 (MQ=255)
cTCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCttt > 1:3159684/1‑76 (MQ=255)
tCAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTg > 2:4407076/1‑76 (MQ=255)
tCAGAGAAAACCCTGGATCTTTCGTGAAATTCAGCATAATCTGGACACTGGGGAGATGCTACCCCCGCAACCttgg > 2:3375719/1‑74 (MQ=255)
cAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTgg > 2:197597/1‑76 (MQ=255)
cAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTgg > 2:6050047/1‑76 (MQ=255)
cAGAGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTgg > 2:7707235/1‑76 (MQ=255)
|
TGACCCGCTTAAAGCCAGAGCTGTGCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGTTGCTGCCCCCGCTGCCTTTGGC > REL606/3338782‑3338928
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
