Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A24 F1 I1 R1
|
18 |
37.8 |
2419812 |
96.5% |
2335118 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,251,892 |
G→A |
P290L (CCG→CTG) |
nusA ← |
transcription elongation factor NusA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,251,892 | 0 | G | A | 100.0%
| 128.0
/ NA
| 34 | P290L (CCG→CTG) | nusA | transcription elongation factor NusA |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (15/19); total (15/19) |
CAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCACCCA > REL606/3251819‑3251964
|
cAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCAGt > 1:258236/1‑76 (MQ=255)
ttACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCAGTGCCa > 1:181329/1‑76 (MQ=255)
ttACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCAGTGCCa < 2:640852/76‑1 (MQ=255)
aCCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCAGTGCCAtt < 2:776114/76‑1 (MQ=255)
cGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCAGTGCCATTGc < 2:13502/76‑1 (MQ=255)
ttCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCAGTGCCATTGCGTTa < 1:191762/76‑1 (MQ=255)
cAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCAGTGNCATTGCGTTAAt < 1:12990/76‑1 (MQ=255)
cAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCAGTGCCATTGCGTTAAt > 2:440947/1‑76 (MQ=255)
aCGNCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGNAGCAACGTCTGCCAGTGCCATTGCGTTAATCa < 2:1127242/76‑1 (MQ=255)
nggCGATATCCCTGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCAGTGCNATTGCGTTAATCAc > 2:174431/2‑76 (MQ=255)
gCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCAGTGCCATTGCGTTAATCACGa < 2:365980/76‑1 (MQ=255)
atatCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCAGTGCCATTGCGTTAATCACGAACt < 2:106164/76‑1 (MQ=255)
cATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCAGTGCCATTGCGTTAATCACGAACTGCGcc > 1:507494/1‑76 (MQ=255)
aTGGTGTGTTTATGTTCATCCACCACGATAGAAGCAACGTCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCg > 1:84909/1‑76 (MQ=255)
ggTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCggg < 2:599001/76‑1 (MQ=255)
tgtgtTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGtt < 2:908363/76‑1 (MQ=255)
tgtgtTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGtt < 1:1104964/76‑1 (MQ=255)
cTTCATCCACCACGATAGAAGCAACGTCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCca < 2:945873/76‑1 (MQ=255)
tCATCCACCACGATAGAAGCAACGTCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCcaca < 1:830782/76‑1 (MQ=255)
ccaccaCGATAGAAGCAACGTCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGAc < 1:428385/76‑1 (MQ=255)
ccaccaCGATAGAAGCAACGTCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGAc > 1:260852/1‑76 (MQ=255)
caccacGATAGAAGCAACGTCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACg < 1:849408/76‑1 (MQ=255)
ccacGTTAGAAGCTACGTGGGCCAGTTCCAGTGCGTTAATCTCGAACTGCGCCGGGTTTTCATCCCACAGGACGat < 2:919135/76‑1 (MQ=255)
cacGATAGAAGCAACGTCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGata > 2:779319/1‑76 (MQ=255)
gATAGAAGCAACGTCTGCCAGTGCCTTTGCGCTAATCACANNCTGCGCCGGGNNACCATCCCACAGGACGATATCg > 1:1084009/1‑76 (MQ=255)
gATAGAAGCAACGTCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCg > 2:10918/1‑76 (MQ=255)
aGAAGCAACGTCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATa > 2:1176549/1‑76 (MQ=255)
aGAAGCAACGTCTGCCAGTGCCATTGCGTTAATCACGAACCGCGCCGGGTTATCATCCCACAGGACGATATCGATa < 1:504193/76‑1 (MQ=255)
aaGCAACGTCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACg < 2:832596/76‑1 (MQ=255)
cAACGTCTGCCAGTGCCATTGCGTTAATCACTAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTc < 2:1181213/76‑1 (MQ=255)
cAACGTCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTc < 2:491186/76‑1 (MQ=255)
aaCGTCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCg < 1:926066/76‑1 (MQ=255)
aaCGGCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCGCAGGACGATATCGATACGCTCg < 1:926048/76‑1 (MQ=255)
gTCTGCCAGAGCCATTGCGTTAANCACGANCTGCGGCGGGTTATNATACCACAGGACGATAACGATACGCTCGCCa > 2:156485/1‑76 (MQ=255)
tCTGCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCAc > 1:253996/1‑76 (MQ=255)
tGCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCAccc > 2:557933/1‑76 (MQ=255)
gCCAGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCACCCa > 1:200571/1‑76 (MQ=255)
|
CAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCACCCA > REL606/3251819‑3251964
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
