Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F1 I1 R1
|
21 |
62.2 |
4089897 |
94.3% |
3856772 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,923,350 |
T→A |
I291N (ATC→AAC) |
rep → |
DNA helicase and single‑stranded DNA‑dependent ATPase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,923,350 | 0 | T | A | 100.0%
| 200.7
/ NA
| 55 | I291N (ATC→AAC) | rep | DNA helicase and single‑stranded DNA‑dependent ATPase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (28/27); total (28/27) |
GCGCTGAAGGTGATTAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTAAAAGTTTTA > REL606/3923277‑3923423
|
gcgcTGAAGGTGATNAAGCTTGAGCAGAACTACCGCTCTTCCTGACGTATTTTGAAAGCGGCGGACATCCTGAACg < 1:1743448/76‑1 (MQ=255)
gcgcTGAAGGTGAATAAGCTTGAGCAGAACTNTCGCTCTACCGGACGTATTTTGAAAGCGGCGANCATCCTGAACg > 1:240204/1‑76 (MQ=255)
gcTGAAGTTGATTAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGcc < 1:437985/76‑1 (MQ=255)
tGAAGGTGATTAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCTGCGAACATCCTGAACGCCaa > 1:2027122/1‑76 (MQ=255)
tGAAGGCGATTAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCaa > 1:1935673/1‑76 (MQ=255)
gAAGGTGATTAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAATATCCTGAACGCCaat > 1:1692919/1‑76 (MQ=255)
gAAGGTGATTAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCaat < 1:980481/76‑1 (MQ=255)
gAAGGTGATTAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCaat > 2:1730200/1‑76 (MQ=255)
aaGGTGATTAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCaata < 1:743528/76‑1 (MQ=255)
aGGTGATTAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCaataa < 2:377035/76‑1 (MQ=255)
tGATTAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATcc < 1:1306592/76‑1 (MQ=255)
gATTAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCg > 1:899251/1‑76 (MQ=255)
aTTAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGc < 2:943326/76‑1 (MQ=255)
tAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCTATAATCCGCAc > 2:700865/1‑76 (MQ=255)
tAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCAc < 1:1569982/76‑1 (MQ=255)
cTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCt < 1:269987/76‑1 (MQ=255)
gAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTg > 1:533971/1‑76 (MQ=255)
aGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGNCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGa < 1:1983657/76‑1 (MQ=255)
cAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGaaa > 2:151884/1‑76 (MQ=255)
cAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGaaa > 2:695706/1‑76 (MQ=255)
cAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGaaa < 2:1379469/76‑1 (MQ=255)
gAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAg > 2:1378573/1‑76 (MQ=255)
tACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTc > 2:487843/1‑76 (MQ=255)
gaCCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTc < 2:949940/75‑1 (MQ=255)
aCCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCt > 2:593907/1‑76 (MQ=255)
aCCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCt > 2:1432143/1‑76 (MQ=255)
aCCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCt < 1:1272007/76‑1 (MQ=255)
aCCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCt < 1:1066774/76‑1 (MQ=255)
ccGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTg > 2:1335245/1‑76 (MQ=255)
cGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGt < 2:62550/76‑1 (MQ=255)
ctTCCGGACGTATTTAGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTtctc > 1:192094/1‑76 (MQ=255)
ttCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTcc > 1:694863/1‑76 (MQ=255)
tCCGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGAGTCTGTTCTCCg > 2:1764159/1‑76 (MQ=255)
ccGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCTAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGa > 1:1704476/1‑76 (MQ=255)
ccGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGa < 1:639631/76‑1 (MQ=255)
ccGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGa < 1:777070/76‑1 (MQ=255)
ccGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGa < 1:1539998/76‑1 (MQ=255)
cGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGaa > 1:1392574/1‑76 (MQ=255)
cGGACGTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTNCTCCGaa < 2:1643763/76‑1 (MQ=255)
gTATTTTGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTggg > 1:419465/1‑76 (MQ=255)
ttttGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTa > 2:1226138/1‑76 (MQ=255)
ttttGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTa < 1:818112/76‑1 (MQ=255)
ttGAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACg > 2:1775801/1‑76 (MQ=255)
gAAAGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGc > 1:1580386/1‑76 (MQ=255)
aaaGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGca < 1:1246290/76‑1 (MQ=255)
aaaGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGca > 2:726659/1‑76 (MQ=255)
aaGCGGCGAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGcac < 1:593908/76‑1 (MQ=255)
cggcgAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACaga < 2:464932/76‑1 (MQ=255)
gAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTa < 2:1443313/76‑1 (MQ=255)
gAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTa < 2:1051164/76‑1 (MQ=255)
gAACATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTa < 1:868612/76‑1 (MQ=255)
aaCATCCTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTaa > 2:1233030/1‑76 (MQ=255)
cTGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTAAAAGttt > 1:1627551/1‑76 (MQ=255)
tGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTAAAAGtttt > 2:1286594/1‑76 (MQ=255)
tGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTAAAAGtttt < 2:1109480/76‑1 (MQ=255)
tGAACGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCC‑AACTGGGTTACGGCACAGAGCTAAAAGTTTTa < 1:1899493/76‑1 (MQ=255)
|
GCGCTGAAGGTGATTAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTAAAAGTTTTA > REL606/3923277‑3923423
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
