Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F1 I1 R1
|
21 |
62.2 |
4089897 |
94.3% |
3856772 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,165,277 |
A→C |
E106A (GAA→GCA) |
rpoC → |
DNA‑directed RNA polymerase subunit beta' |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,165,277 | 0 | A | C | 98.0%
| 206.0
/ NA
| 52 | E106A (GAA→GCA) | rpoC | DNA‑directed RNA polymerase subunit beta' |
| Reads supporting (aligned to +/- strand): ref base A (1/0); major base C (25/25); minor base G (0/1); total (26/26) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATAT > REL606/4165204‑4165349
|
ggCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCAc > 1:349780/1‑76 (MQ=255)
cATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCt > 1:656161/1‑76 (MQ=255)
cATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCt < 2:1370177/76‑1 (MQ=255)
tgtgAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTccc > 1:1080669/1‑76 (MQ=255)
gtgAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTcccc > 2:1404195/1‑76 (MQ=255)
tgAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCg < 1:1325220/76‑1 (MQ=255)
tgAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCg < 2:636316/76‑1 (MQ=255)
gagaAGTGCGGCGTTGAAGTGACCCTGAGTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGa < 1:324024/76‑1 (MQ=255)
gagaAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGa < 1:215835/76‑1 (MQ=255)
agaAGTGCGGCGTTNAAGTGACCCAGACTAAAGTACGCCGTGTGCGTATGGGCCACATCGGACTGGCTTCCCCGAc < 1:1750708/76‑1 (MQ=255)
agaAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGAc < 1:918885/76‑1 (MQ=255)
tGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTgcgc < 1:975369/76‑1 (MQ=255)
gcggcgTTGAAGTGACCCAGGCTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCATCCCCGACTGCGca > 1:98360/1‑76 (MQ=255)
ggcgTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGcaca > 1:833147/1‑76 (MQ=255)
ttGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTg > 1:905861/1‑76 (MQ=255)
tGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTgg > 1:1449023/1‑76 (MQ=255)
tGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTgg > 2:1336485/1‑76 (MQ=255)
tGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCt < 1:438420/76‑1 (MQ=255)
gACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTg < 1:976797/76‑1 (MQ=255)
aCCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGa > 1:1645793/1‑76 (MQ=255)
aCCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGa < 1:359296/76‑1 (MQ=255)
cccAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGaa < 2:1509730/76‑1 (MQ=255)
aGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATc < 1:979437/76‑1 (MQ=255)
gACTAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCg < 2:689643/76‑1 (MQ=255)
aCTAAAGTACGCCGTGAGCGTATGGGCTACAACGNACTGACTNACNCANCTGCTCACCTCTGGTTCCTGAAATCGc > 1:1759009/1‑76 (MQ=255)
tAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGTGGAACTCTTGTTTCTGTCATCGcgt > 2:637416/1‑74 (MQ=255)
tAAAGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGGAAACGCTg > 2:637442/1‑76 (MQ=255)
aaaGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGc > 2:587231/1‑76 (MQ=255)
aaaGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGc > 2:1104228/1‑76 (MQ=255)
aaaGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGc < 2:1760277/76‑1 (MQ=255)
aaaGTACGCCGTGAGCGTACGGGCCACATCGNCCTGACGTCACCGACTGCGCACATCTGGTTCGNGAAATCGCTGc > 1:245287/1‑76 (MQ=255)
aaGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGcc < 1:1701237/76‑1 (MQ=255)
aGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCg < 2:1037385/76‑1 (MQ=255)
aGTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCg < 2:1538203/76‑1 (MQ=255)
gTACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCCCTGCCGt > 1:949931/1‑76 (MQ=255)
tACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTc < 1:1591069/76‑1 (MQ=255)
tACGCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTc > 2:1238824/1‑76 (MQ=255)
gCCGTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCg > 1:724989/1‑76 (MQ=255)
gCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCg > 1:1461963/1‑76 (MQ=255)
gTGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTAt < 2:1771586/76‑1 (MQ=255)
tGAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATc > 1:550686/1‑76 (MQ=255)
gAGCGTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCg < 1:968257/76‑1 (MQ=255)
gTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTct < 2:1301546/76‑1 (MQ=255)
gTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTct > 2:1293231/1‑76 (MQ=255)
gTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTct > 1:1685071/1‑76 (MQ=255)
gTATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTAGCTGTct < 1:1914078/76‑1 (MQ=255)
tATGGGCCACATCGCACTGGCTTCCNCGACTGCGCACATCTGGTTCTNGAATTCGCTGCCGTCCCGTATCGGTctg < 1:285663/76‑1 (MQ=255)
tATGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTctg < 1:1628968/76‑1 (MQ=255)
tATGCGCCACATCGCACTGGCTTCACCGACTGCGCACATCTGGTTCCTGAAACCGCTGCCGTCCCGTATCGGTctg < 2:1454120/76‑1 (MQ=255)
aTGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTAGCGGGctgc > 2:1917815/1‑76 (MQ=255)
tGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTctgct > 1:1806290/1‑76 (MQ=255)
tGGGCCACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGAATCGGTctgct > 2:1824136/1‑76 (MQ=255)
gCCACATCGCGCTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTgctgct < 2:1841043/76‑1 (MQ=255)
ccACATCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTc < 2:833146/76‑1 (MQ=255)
caTCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGat > 1:171005/1‑76 (MQ=255)
caTCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCCGCCTGCTGCTCGat > 2:499828/1‑76 (MQ=255)
tCGCACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGatat < 2:758197/76‑1 (MQ=255)
|
GGCGTCATCTGTGAGAAGTGCGGCGTTGAAGTGACCCAGACTAAAGTACGCCGTGAGCGTATGGGCCACATCGAACTGGCTTCCCCGACTGCGCACATCTGGTTCCTGAAATCGCTGCCGTCCCGTATCGGTCTGCTGCTCGATAT > REL606/4165204‑4165349
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
