Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A132 F1 I1 R1
|
21 |
265.3 |
16744490 |
95.4% |
15974243 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,108,360 |
T→C |
N48S (AAC→AGC) |
metJ ← |
transcriptional repressor protein MetJ |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,108,360 | 0 | T | C | 99.3%
| 1146.5
/ ‑4.0
| 278 | N48S (AAC→AGC) | metJ | transcriptional repressor protein MetJ |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (123/153); total (124/154) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 |
Only 100 of 291 total aligned reads displayed.
CGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCT > REL606/4108287‑4108432
|
cGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGgctg > 1:4194667/1‑76 (MQ=255)
ggCAAAGGATGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGgctgg > 2:3382863/1‑75 (MQ=255)
cAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTc < 2:3110197/76‑1 (MQ=255)
cAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTc < 2:1998143/76‑1 (MQ=255)
ggTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCt < 2:4136356/76‑1 (MQ=255)
gTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTg < 2:4650592/76‑1 (MQ=255)
ttGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTgg < 2:5690760/76‑2 (MQ=255)
ttGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTga > 2:5950157/1‑76 (MQ=255)
ttGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTga < 2:5206931/76‑1 (MQ=255)
tGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCa < 2:2377576/70‑1 (MQ=255)
tGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTgac > 1:4411473/1‑76 (MQ=255)
tGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTgac < 1:2893310/76‑1 (MQ=255)
tGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTgac < 1:7155967/76‑1 (MQ=255)
tGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTgac > 1:7947410/1‑76 (MQ=255)
gCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTgacg < 1:7652723/76‑1 (MQ=255)
gCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTgacg < 1:3625227/76‑1 (MQ=255)
gCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTgacg < 2:4604680/76‑1 (MQ=255)
cccGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCATCTgtcga > 2:6307667/1‑76 (MQ=255)
ccGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTgacgac < 2:3220347/76‑1 (MQ=255)
cGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGacgacg > 1:5646261/1‑76 (MQ=255)
ggTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGAcgc > 1:4760779/1‑76 (MQ=255)
tAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACgcgt > 2:8308517/1‑76 (MQ=255)
tAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACgcgt > 1:3936993/1‑76 (MQ=255)
aaaGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGAcgcggg > 1:5921784/1‑74 (MQ=255)
aaGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACgcgtgc > 1:1972551/1‑76 (MQ=255)
aGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACgcgtgcg < 1:5056477/76‑1 (MQ=255)
aGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACgcgtgcg > 2:7130111/1‑76 (MQ=255)
cATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTc < 2:7619364/76‑1 (MQ=255)
cATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTc > 1:3111774/1‑76 (MQ=255)
aTGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCa > 2:6833194/1‑76 (MQ=255)
aTGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGGGCGTTCa > 2:6653883/1‑76 (MQ=255)
tGCAGAAACGCTTCGCGCAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGAGTTCAt < 1:6094194/76‑1 (MQ=39)
tGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCAt < 1:1758621/76‑1 (MQ=255)
gCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATc < 2:6021795/76‑1 (MQ=255)
aGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCgg > 1:3736349/1‑76 (MQ=255)
gAAACGCTTCGCACAGCAGCTTGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGt < 2:2067287/76‑1 (MQ=255)
gAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGt < 2:2169366/76‑1 (MQ=255)
aaaCGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTg < 2:2321409/76‑1 (MQ=255)
aaCGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGa > 2:5831610/1‑76 (MQ=255)
aCGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAg < 2:1672527/76‑1 (MQ=255)
gCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGa < 1:6414480/76‑1 (MQ=255)
gCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGa > 2:6577863/1‑76 (MQ=255)
cTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAgggt > 1:6725971/1‑74 (MQ=255)
tCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGAttt < 1:511781/76‑1 (MQ=255)
cGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGAtttt > 1:7759760/1‑76 (MQ=255)
cGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGAtttt < 2:124956/76‑1 (MQ=255)
cGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGAtttt > 1:3529361/1‑76 (MQ=255)
gCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGAttttt < 1:1161307/76‑1 (MQ=255)
gCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGAttttt < 2:4935170/76‑1 (MQ=255)
cacaGCAGCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGGGAGGATTTTTa > 2:6939169/1‑76 (MQ=255)
cagcagCTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAac < 1:7669841/76‑1 (MQ=255)
agcagcTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAaca < 1:1673541/76‑1 (MQ=255)
cagcTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACAcc > 2:2560370/1‑76 (MQ=255)
cagcTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGGGCGTTCATCGGTGAGGATTTTTAAAAcc > 2:1214858/1‑76 (MQ=255)
gcTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCtt < 2:2745798/76‑1 (MQ=255)
gcTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCtt < 2:8031523/76‑1 (MQ=255)
gcTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCtt < 1:1513652/76‑1 (MQ=255)
cTCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTa < 2:6287262/76‑1 (MQ=255)
tCGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTaa < 2:4397747/76‑1 (MQ=255)
cGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAag < 1:2380311/76‑1 (MQ=255)
cGCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAag < 2:5539373/76‑1 (MQ=255)
gCTGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAaga < 1:2695806/76‑1 (MQ=255)
tGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAgg > 1:5208764/1‑76 (MQ=255)
tGTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGGGAGGATTTTTAACACCTTAAgggg > 1:5392781/1‑76 (MQ=255)
gTTGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGa < 2:2671207/76‑1 (MQ=255)
ttGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGaa < 2:3705491/76‑1 (MQ=255)
ttGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGaa < 1:872449/76‑1 (MQ=255)
ttGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGaa < 1:1604379/76‑1 (MQ=255)
ttGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGaa < 1:6664178/76‑1 (MQ=255)
tGGTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTCAGAGGAAt > 2:1305558/1‑76 (MQ=255)
gTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATgg < 1:2502782/76‑1 (MQ=255)
gTAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATgg < 1:752011/76‑1 (MQ=255)
tAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGa < 2:3070394/76‑1 (MQ=255)
tAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGa > 1:4941791/1‑76 (MQ=255)
tAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGa < 1:1208950/76‑1 (MQ=255)
tAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGa < 1:8158783/76‑1 (MQ=255)
tAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGa < 2:7824753/76‑1 (MQ=255)
tAGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGa < 1:7222621/76‑1 (MQ=255)
aGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGaa < 1:2213939/76‑1 (MQ=255)
aGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGaa > 1:3809239/1‑76 (MQ=255)
aGCGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGaa > 1:6172538/1‑76 (MQ=255)
cGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGGTTTTTAACACCTTAAGAGGAATGGAAAc > 1:5861957/1‑76 (MQ=255)
cGTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAAc > 1:6251508/1‑76 (MQ=255)
gTGACGCAGGTTGTTCACCTGACGACGCGTGCGGTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAAcc < 1:3966562/76‑1 (MQ=255)
gTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAAcc > 1:1295451/1‑76 (MQ=255)
gTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAAcc < 2:3526240/76‑1 (MQ=255)
gTGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAAcc < 2:3250869/76‑1 (MQ=255)
tGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCg > 2:5430398/1‑76 (MQ=255)
tGACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCg < 1:4644784/76‑1 (MQ=255)
gACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGt > 1:996421/1‑76 (MQ=255)
gACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGt < 1:4515807/76‑1 (MQ=255)
gACGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGt > 2:174794/1‑76 (MQ=255)
aCGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTa < 2:4729139/76‑1 (MQ=255)
cGCAGGCTGTTCACCTGACGACGCGTTCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTaa < 1:8364799/76‑1 (MQ=255)
cGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTaa < 2:2956394/76‑1 (MQ=255)
cGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTaa < 2:7433413/76‑1 (MQ=255)
cGCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTaa < 2:6059/76‑1 (MQ=255)
gCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAAt > 1:6503787/1‑76 (MQ=255)
gCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAAt < 1:695004/76‑1 (MQ=255)
gCAGGCTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAAt < 2:3904172/76‑1 (MQ=255)
|
CGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCT > REL606/4108287‑4108432
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
