Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A73 F1 I1 R1
|
21 |
62.0 |
3993596 |
96.1% |
3837845 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
1,588,754 |
A→C |
F50L (TTT→TTG) |
eamA ← |
cysteine and O‑acetyl‑L‑serine efflux system |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 1,588,754 | 0 | A | C | 97.0%
| 261.8
/ ‑1.9
| 67 | F50L (TTT→TTG) | eamA | cysteine and O‑acetyl‑L‑serine efflux system |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (31/34); total (32/35) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.63e-01 |
AAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCGGTGGCATGTTATGAAGC > REL606/1588681‑1588826
|
aagCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACcaa > 2:213602/1‑76 (MQ=255)
agCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACcaaa < 1:263070/76‑1 (MQ=255)
gCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACcaaaa < 2:892673/76‑1 (MQ=255)
cAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACcaaaaa > 1:472902/1‑76 (MQ=255)
aaaCTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAg > 2:170602/1‑76 (MQ=255)
aaCTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGa > 1:359544/1‑76 (MQ=255)
aCTGCGCAAAACTGATGGTTAATCCATACCCCGGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGAt < 2:239372/76‑1 (MQ=255)
aCTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGAt < 1:1548183/76‑1 (MQ=255)
aCTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGAt < 2:836925/76‑1 (MQ=255)
cTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATa > 2:20865/1‑76 (MQ=255)
tGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAg < 2:471717/76‑1 (MQ=255)
tGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAg < 2:1786090/76‑1 (MQ=255)
gcgcAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGc < 1:119574/76‑1 (MQ=255)
aaaaCTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGa > 1:937818/1‑76 (MQ=255)
aaaaCTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGa > 1:1884089/1‑76 (MQ=255)
aaaCTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGaa < 2:525639/76‑1 (MQ=255)
aaaCTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGaa > 2:1803795/1‑76 (MQ=255)
aaCTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGaaa > 2:1239211/1‑76 (MQ=255)
aaCTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGaaa < 2:775846/76‑1 (MQ=255)
cTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAg > 1:450637/1‑76 (MQ=255)
gATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCg > 1:674986/1‑76 (MQ=255)
aTGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGa > 2:1338722/1‑76 (MQ=255)
tGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGAc < 2:783732/76‑1 (MQ=255)
ggTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGAcc > 2:1043908/1‑76 (MQ=255)
ggTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGAcc > 2:498119/1‑76 (MQ=255)
gTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCa < 1:466830/76‑1 (MQ=255)
ttAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCaa < 1:10820/76‑1 (MQ=255)
ttAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCaa < 2:964462/76‑1 (MQ=255)
ttAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCaa > 1:1743617/1‑76 (MQ=255)
tAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAAc > 1:1806111/1‑76 (MQ=255)
tAACCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAAc > 1:1875320/1‑76 (MQ=255)
aaTCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACa < 2:1882983/76‑1 (MQ=255)
aTCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACAt > 1:1575207/1‑76 (MQ=255)
aTCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAACAt < 1:1073708/76‑1 (MQ=255)
tCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATa > 2:822003/1‑76 (MQ=255)
ccATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATaa < 2:178883/76‑1 (MQ=255)
cATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATaaa > 1:1760572/1‑76 (MQ=255)
cATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATaaa > 2:1547888/1‑76 (MQ=255)
aTACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAg < 2:611709/76‑1 (MQ=255)
aCCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAgcg < 2:1905735/76‑1 (MQ=255)
aCCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAgcg > 1:1917546/1‑76 (MQ=255)
cagcagCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCaaa > 1:1468134/1‑76 (MQ=255)
agcagcAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAAc < 2:1292711/76‑1 (MQ=255)
agcagcAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAAc > 2:879087/1‑76 (MQ=255)
gcagcaAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAAcc > 2:1819741/1‑76 (MQ=255)
cagcaAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCg < 2:1397031/76‑1 (MQ=255)
gcaAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCgac > 2:1581324/1‑74 (MQ=255)
caAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGcc < 1:1041164/76‑1 (MQ=255)
caAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGcc < 1:1343598/76‑1 (MQ=255)
caAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGcc < 1:711718/76‑1 (MQ=255)
aaTTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAg < 2:411182/76‑1 (MQ=255)
aaTTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAg > 1:1042641/1‑76 (MQ=255)
aaTTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAg < 1:1337580/76‑1 (MQ=255)
aTTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGc > 1:693426/1‑76 (MQ=255)
aGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTa < 2:488638/76‑1 (MQ=255)
tGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGc > 1:1291932/1‑76 (MQ=255)
tGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGc > 1:1329446/1‑76 (MQ=255)
tACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCggg > 2:386309/1‑75 (MQ=255)
tACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCGGt < 1:622647/76‑1 (MQ=255)
ttCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCGGTGGCa > 2:548001/1‑76 (MQ=255)
ttCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCGGTGGCa > 2:1942009/1‑76 (MQ=255)
ggTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCGGTGGCATGt < 1:950541/76‑1 (MQ=255)
tCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCGGTGGCATGTTa < 2:1036576/76‑1 (MQ=255)
tCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCGGTGGCATGTTa < 2:1254282/76‑1 (MQ=255)
tCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCGGTGGCATGTTa < 1:553643/76‑1 (MQ=255)
cGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCGGTGGCATGTTAt < 2:1016287/76‑1 (MQ=255)
gTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCGGTGGCATGTTATg < 2:278937/76‑1 (MQ=255)
tGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCGGTGGCATGTTATGa > 2:1098788/1‑76 (MQ=255)
tGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCGGTGGCATGTTATGa < 2:1340147/76‑1 (MQ=255)
gCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCGGTGGCATGTTATGaa < 1:1687428/76‑1 (MQ=255)
cGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCGGTGGCATGTTATGAAg < 2:1968392/76‑1 (MQ=255)
gACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCGGTGGCATGTTATGAAGc < 2:832729/76‑1 (MQ=255)
|
AAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCGGTGGCATGTTATGAAGC > REL606/1588681‑1588826
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
