Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A107 F1 I1 R1
|
19 |
326.9 |
20855348 |
93.1% |
19416328 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,912,192 |
G→A |
G24E (GGG→GAG) |
ilvL → |
ilvG operon leader peptide |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,912,192 | 0 | G | A | 99.0%
| 1186.4
/ ‑0.1
| 300 | G24E (GGG→GAG) | ilvL | ilvG operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base A (167/130); total (168/132) |
| Fisher's exact test for biased strand distribution p-value = 5.84e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01 |
Only 100 of 310 total aligned reads displayed.
AAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCG > REL606/3912119‑3912264
|
aaaTGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGc < 1:9622105/76‑1 (MQ=255)
aTGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTg < 1:5947418/76‑1 (MQ=255)
aTGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTg > 1:2179301/1‑76 (MQ=255)
aTGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTg > 2:6973649/1‑76 (MQ=255)
tGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGc > 2:703839/1‑76 (MQ=255)
gACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCa < 1:9960860/76‑1 (MQ=255)
cAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACt < 2:1542977/76‑1 (MQ=255)
cAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACt > 2:4646818/1‑76 (MQ=255)
cAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACt < 2:1404177/76‑1 (MQ=255)
cAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACt < 2:7196049/76‑1 (MQ=255)
cAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACt > 2:9178218/1‑76 (MQ=255)
aGCCCTTCTACGAGTGCTTAGCTTGGTAGTGTTTAGAGTGGTGTTGATTATTATCCCACGGTGCTAGGCTGCAcct > 2:6009791/1‑74 (MQ=255)
aGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACtt > 2:5215478/1‑76 (MQ=255)
aGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACtt < 2:2179301/76‑1 (MQ=255)
cccTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTgg < 1:2893820/76‑1 (MQ=255)
cccTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTgg > 1:829684/1‑76 (MQ=255)
cTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGAc < 1:7534390/76‑1 (MQ=255)
cTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGAc < 2:5350998/76‑1 (MQ=255)
cTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGAc > 2:8452794/1‑76 (MQ=255)
cTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAg > 1:8362832/1‑76 (MQ=255)
cTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAg > 1:5007037/1‑76 (MQ=255)
cTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAg < 2:5740905/76‑1 (MQ=255)
cTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAg < 2:8690133/76‑1 (MQ=255)
tACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAgg < 1:5647319/76‑1 (MQ=255)
tACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAgg > 2:1932338/1‑76 (MQ=255)
tACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAgg > 1:383058/1‑76 (MQ=255)
tACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAgg > 2:7337996/1‑76 (MQ=255)
tACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAgg > 2:834199/1‑76 (MQ=255)
aCGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGa > 1:7842605/1‑76 (MQ=255)
aCGAGCGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGa > 2:9408193/1‑76 (MQ=255)
cGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGaa < 1:489238/76‑1 (MQ=255)
gAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGaaa < 2:6867609/76‑1 (MQ=255)
aGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAg < 1:5346065/76‑1 (MQ=255)
aGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAg < 1:3504010/76‑1 (MQ=255)
aGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAg < 2:7725491/76‑1 (MQ=255)
gTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAgg < 1:110781/76‑1 (MQ=255)
gATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCt < 1:6652690/76‑1 (MQ=255)
aTTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCtt > 2:3048956/1‑76 (MQ=255)
ttAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGGCGAGGAAAGGCTTa < 1:4408574/76‑1 (MQ=255)
tAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTag > 2:8083574/1‑76 (MQ=255)
tAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTag > 1:9815917/1‑76 (MQ=255)
aGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTaga < 2:1789307/76‑1 (MQ=255)
gCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTagag > 1:7338599/1‑76 (MQ=255)
ccTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAgaga > 1:6260688/1‑76 (MQ=255)
cTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGAt > 1:527388/1‑76 (MQ=255)
cTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGAt > 1:8441357/1‑76 (MQ=255)
cTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGAt > 1:4209487/1‑76 (MQ=255)
tGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATc > 2:4313261/1‑76 (MQ=255)
ggTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCa > 1:10329737/1‑76 (MQ=255)
ggTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCa < 1:571369/76‑1 (MQ=255)
cGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGc > 1:9270415/1‑76 (MQ=255)
cGTGATTAGCGTGGGGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGc > 2:3167280/1‑76 (MQ=255)
gATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCtt < 2:1607199/76‑1 (MQ=255)
gATTAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCtt < 2:3525043/76‑1 (MQ=255)
ttAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTaa < 2:6405172/76‑1 (MQ=255)
ttAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTaa > 2:4399463/1‑76 (MQ=255)
ttAGCGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTaa > 2:2265098/1‑76 (MQ=255)
cGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGaa < 1:4961768/76‑1 (MQ=255)
cGTGGTGGTGATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGaa > 2:3318181/1‑76 (MQ=255)
gtggtggtgATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAAc > 1:1427249/1‑76 (MQ=255)
tggtggtgATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACt > 1:6962495/1‑76 (MQ=255)
ggtggtgATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTa > 1:5108783/1‑76 (MQ=255)
gtggtgATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTaa > 2:5922185/1‑76 (MQ=255)
gtggtgATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTaa < 1:3577372/76‑1 (MQ=255)
gtggtgATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTaa < 2:3378835/76‑1 (MQ=255)
tggtgATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAg > 2:4141500/1‑76 (MQ=255)
tggtgATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAg < 2:4726453/76‑1 (MQ=255)
tggtgATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAg < 1:8871317/76‑1 (MQ=255)
tggtgATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAg > 2:10358037/1‑76 (MQ=255)
ggtgATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGa > 1:2977433/1‑76 (MQ=255)
gtgATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGAc < 1:7215171/76‑1 (MQ=255)
tgATTATTATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGAcc > 2:4835383/1‑76 (MQ=255)
attattATCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGAcccc < 1:8704640/76‑1 (MQ=255)
ttattaTCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGAccccc > 1:9152325/1‑76 (MQ=255)
ttattaTCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGAccccc < 2:9013510/76‑1 (MQ=255)
attatCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGc < 2:8852272/76‑1 (MQ=255)
attatCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGc > 2:5444176/1‑76 (MQ=255)
attatCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGc < 2:786407/76‑1 (MQ=255)
attatCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGc > 2:1139580/1‑76 (MQ=255)
ttatCCCACCGTGCGAGGCTGCCCTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCa > 1:2546990/1‑76 (MQ=255)
ttatCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCa < 1:4668223/76‑1 (MQ=255)
tatCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCAc > 1:4237850/1‑76 (MQ=255)
atCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCAcc > 1:9041019/1‑76 (MQ=255)
atCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCAcc > 1:1987670/1‑76 (MQ=255)
atCCCACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCAcc > 1:10001469/1‑76 (MQ=255)
ccccACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCg < 1:2760852/75‑1 (MQ=255)
cccACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGa > 1:6035724/1‑76 (MQ=255)
cccACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGa > 2:2671806/1‑76 (MQ=255)
cccACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGa > 1:6461010/1‑76 (MQ=255)
cccACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGa < 1:3248205/76‑1 (MQ=255)
ccACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGaa < 1:3305694/76‑1 (MQ=255)
ccccGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGaaa < 1:1850632/74‑1 (MQ=255)
cACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGaaa < 1:4568933/76‑1 (MQ=255)
cACCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGaaa > 2:4482385/1‑76 (MQ=255)
aCCGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAg < 1:4515420/76‑1 (MQ=255)
cGTGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGt < 2:6609226/76‑1 (MQ=255)
tGCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTcc > 1:4441924/1‑76 (MQ=255)
gCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCg < 2:1332359/76‑1 (MQ=255)
gCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCg > 1:4120700/1‑76 (MQ=255)
gCGAGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACACCCGCACCGAAAGGTCCg > 2:2501654/1‑76 (MQ=255)
|
AAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCG > REL606/3912119‑3912264
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
