breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 398,586 G→T R220L (CGT→CTT)  secF → protein export protein SecF
MC JC 433,584 Δ16 bp coding (79‑94/273 nt) hupB → HU, DNA‑binding transcriptional regulator, beta subunit
RA 1,329,516 C→T H33Y (CAC→TAC)  topA → DNA topoisomerase I
JC 1,733,297 (CGTATGAA)1→2 coding (333/1413 nt) pykF → pyruvate kinase
JC 2,103,915 (CCAG)7→6 coding (182‑185/216 nt) ECB_01992 → hypothetical protein
RA 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein
RA 2,213,060 A→T I165N (ATC→AAC)  fruK ← 1‑phosphofructokinase
RA 2,250,824 T→C H640R (CAC→CGC)  napA ← nitrate reductase, periplasmic, large subunit
RA 2,297,789 Δ1 bp coding (1294/1359 nt) glpT ← sn‑glycerol‑3‑phosphate transporter
RA 3,145,986 C→G I41M (ATC→ATG rpoD → RNA polymerase sigma factor
RA 3,535,528 (GCTG)3→4 coding (448/597 nt) yhhF → predicted methyltransferase
RA 3,762,741 A→T K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,875,632 (T)7→8 intergenic (‑66/+287) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
MC JC 3,894,997 Δ6,934 bp IS150‑mediated rbsD[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
RA 3,912,192 G→A G24E (GGG→GAG)  ilvL → ilvG operon leader peptide
RA 4,097,495 +CC coding (78/846 nt) glpF ← glycerol facilitator
RA 4,163,752 T→A I966N (ATC→AAC)  rpoB → DNA‑directed RNA polymerase subunit beta
RA 4,504,889 (A)8→7 intergenic (+633/‑92) insB‑25 → / → ECB_04162 b4563(b4576); IS1 protein insB/hypothetical protein
JC JC 4,524,522 IS186 (+) +6 bp coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ REL606 546978–547700 593371 45672–46394 148 [139] [144] 152 [insB‑6]–[fepA] [insB‑6],insA‑6,nmpC,ybcR,ybcS,ybcT,ybcU,ECB_00510,nohB,ECB_00512,ECB_00513,ECB_00514,ECB_00515,ECB_00516,ECB_00517,appY,ompT,envY,ybcH,nfrA,nfrB,nfrB,ECB_00524,yhhI,ECB_00526,ECB_00527,ECB_00528,ECB_00529,ECB_00530,cusS,cusR,cusC,ylcC,cusB,cusA,pheP,ybdG,nfnB,ybdF,ybdJ,ybdK,insJ‑1,insK‑1,insB‑7,insA‑7,insK‑1,hokE,insL‑3,entD,[fepA]
* * ÷ REL606 593576 619837–619116 25541–26262 150 [144] [143] 147 [fepA]–insA‑8 [fepA],fes,ybdZ,entF,fepE,fepC,fepG,fepD,ybdA,fepB,entC,entE,entB,entA,ybdB,cstA,cstA,ybdD,ybdH,ybdL,ybdM,ybdN,insB‑8,insA‑8