breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
RA | 398,586 | G→T | R220L (CGT→CTT) | secF → | protein export protein SecF |
MC JC | 433,584 | Δ16 bp | coding (79‑94/273 nt) | hupB → | HU, DNA‑binding transcriptional regulator, beta subunit |
RA | 1,329,516 | C→T | H33Y (CAC→TAC) | topA → | DNA topoisomerase I |
JC | 1,733,297 | (CGTATGAA)1→2 | coding (333/1413 nt) | pykF → | pyruvate kinase |
JC | 2,103,915 | (CCAG)7→6 | coding (182‑185/216 nt) | ECB_01992 → | hypothetical protein |
RA | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
RA | 2,213,060 | A→T | I165N (ATC→AAC) | fruK ← | 1‑phosphofructokinase |
RA | 2,250,824 | T→C | H640R (CAC→CGC) | napA ← | nitrate reductase, periplasmic, large subunit |
RA | 2,297,789 | Δ1 bp | coding (1294/1359 nt) | glpT ← | sn‑glycerol‑3‑phosphate transporter |
RA | 3,145,986 | C→G | I41M (ATC→ATG) | rpoD → | RNA polymerase sigma factor |
RA | 3,535,528 | (GCTG)3→4 | coding (448/597 nt) | yhhF → | predicted methyltransferase |
RA | 3,762,741 | A→T | K662I (AAA→ATA) | spoT → | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
RA | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
MC JC | 3,894,997 | Δ6,934 bp | IS150‑mediated | rbsD–[yieO] | rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO] |
RA | 3,912,192 | G→A | G24E (GGG→GAG) | ilvL → | ilvG operon leader peptide |
RA | 4,097,495 | +CC | coding (78/846 nt) | glpF ← | glycerol facilitator |
RA | 4,163,752 | T→A | I966N (ATC→AAC) | rpoB → | DNA‑directed RNA polymerase subunit beta |
RA | 4,504,889 | (A)8→7 | intergenic (+633/‑92) | insB‑25 → / → ECB_04162 | b4563(b4576); IS1 protein insB/hypothetical protein |
JC JC | 4,524,522 | IS186 (+) +6 bp | coding (494‑499/549 nt) | fimA → | major type 1 subunit fimbrin (pilin) |
Unassigned missing coverage evidence | ||||||||||
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seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | REL606 | 546978–547700 | 593371 | 45672–46394 | 148 [139] | [144] 152 | [insB‑6]–[fepA] | [insB‑6],insA‑6,nmpC,ybcR,ybcS,ybcT,ybcU,ECB_00510,nohB,ECB_00512,ECB_00513,ECB_00514,ECB_00515,ECB_00516,ECB_00517,appY,ompT,envY,ybcH,nfrA,nfrB,nfrB,ECB_00524,yhhI,ECB_00526,ECB_00527,ECB_00528,ECB_00529,ECB_00530,cusS,cusR,cusC,ylcC,cusB,cusA,pheP,ybdG,nfnB,ybdF,ybdJ,ybdK,insJ‑1,insK‑1,insB‑7,insA‑7,insK‑1,hokE,insL‑3,entD,[fepA] |
* | * | ÷ | REL606 | 593576 | 619837–619116 | 25541–26262 | 150 [144] | [143] 147 | [fepA]–insA‑8 | [fepA],fes,ybdZ,entF,fepE,fepC,fepG,fepD,ybdA,fepB,entC,entE,entB,entA,ybdB,cstA,cstA,ybdD,ybdH,ybdL,ybdM,ybdN,insB‑8,insA‑8 |