breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 241 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6062,515,9090AC58.5% 45.8 / 109.0 82V260G (GTG→GGG) ypfIpredicted hydrolase
*REL606720,4660GC57.8% 0.6 / 60.9 45A23P (GCC→CCC) phrBdeoxyribodipyrimidine photolyase, FAD‑binding
*REL6061,854,4010AC48.2% 97.9 / 65.3 112K88N (AAA→AACyeaOhypothetical protein
*REL6061,747,9030GT48.2% 372.3 / inf 523A8S (GCA→TCA) ydiLhypothetical protein
*REL6064,180,2490TG46.1% 195.4 / 65.2 154G24G (GGT→GGGyjaHhypothetical protein
*REL6064330TG44.5% 139.1 / 68.3 119V33G (GTG→GGG) thrAbifunctional aspartokinase I/homeserine dehydrogenase I
*REL6062,376,3480TG44.5% 248.3 / 65.2 191T63P (ACC→CCC) folCbifunctional folylpolyglutamate synthase/ dihydrofolate synthase
*REL6064,392,5560CT44.3% 189.3 / 176.1 228L43L (CTA→TTA) aidBisovaleryl CoA dehydrogenase
*REL6063,774,9140CG39.5% 316.2 / 187.3 215A20P (GCC→CCC) yicJpredicted transporter
*REL606895,8560GC39.0% 59.0 / 30.4 41A26P (GCC→CCC) ECB_00836putative Regulatory protein
*REL6064,429,2820AC38.6% 241.7 / 11.9 129T472P (ACC→CCC) ytfNhypothetical protein
*REL6064,111,4290TG37.7% 190.6 / 21.0 106G496G (GGT→GGGmetLbifunctional aspartate kinase II/homoserine dehydrogenase II
*REL6063,378,8850TG37.1% 627.5 / 94.7 313T129P (ACC→CCC) rplB50S ribosomal protein L2
*REL6061,655,4580TG35.8% 567.0 / 176.8 321intergenic (‑360/‑164)pntA/ydgHNAD(P) transhydrogenase subunit alpha/hypothetical protein
*REL606159,6540AC35.7% 572.3 / 51.2 286G27G (GGT→GGGyadNpredicted fimbrial‑like adhesin protein
*REL606931,3140TC34.8% 182.8 / 51.7 139E84G (GAG→GGG) ybjEpredicted transporter
*REL606955,0350AC34.8% 498.5 / 13.2 211T192P (ACC→CCC) lolAouter‑membrane lipoprotein carrier protein precursor
*REL6062,441,7050TG34.5% 216.1 / 12.1 114T139P (ACC→CCC) ypdGpredicted enzyme IIC component of PTS
*REL6061,118,2090GT34.5% 68.0 / 47.0 58intergenic (‑444/‑311)csgD/csgBDNA‑binding transcriptional activator in two‑component regulatory system/curlin nucleator protein, minor subunit in curli complex
*REL6063,230,8560GC34.4% 87.9 / 28.4 61A77P (GCA→CCA) yraNhypothetical protein

Marginal new junction evidence (lowest skew 10 of 70 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 35355150 (0.000)142 (0.470)
+GGCT
47/116 5.2 100% coding (435/597 nt) yhhF predicted methyltransferase
?REL606 3535516 = 0 (0.000)coding (436/597 nt) yhhF predicted methyltransferase
* ? REL606 = 3582336356 (1.130)9 (0.030) 7/140 20.7 2.8% coding (356/1224 nt) yhiS hypothetical protein
?REL606 = 3582485 289 (0.980)coding (505/1224 nt) yhiS hypothetical protein
* ? REL606 = 1506094344 (1.090)10 (0.030) 7/140 20.7 4.0% coding (157/201 nt) ECB_01417 hypothetical protein
?REL606 = 1506258 164 (0.560)intergenic (+120/‑120) ECB_01417/ydcC hypothetical protein/hypothetical protein
* ? REL606 2041682 =268 (0.850)6 (0.020) 5/128 21.2 2.2% coding (1139/1428 nt) wzx O antigen translocase (Wzx)
?REL606 2041834 = 294 (1.090)coding (987/1428 nt) wzx O antigen translocase (Wzx)
* ? REL606 1405596 =236 (0.750)13 (0.040) 6/140 21.4 5.6% coding (377/984 nt) ydaN zinc transporter
?REL606 2034535 = NA (NA)noncoding (559/768 nt) IS1 repeat region
* ? REL606 2330426 =269 (0.850)7 (0.020) 6/144 21.7 2.7% coding (1201/1212 nt) elaD predicted enzyme
?REL606 2330659 = 250 (0.820)intergenic (+222/‑312) elaD/yfbL predicted enzyme/putative aminopeptidase; b2271_1
* ? REL606 1405596 =236 (0.750)31 (0.110) 5/140 22.1 12.3% coding (377/984 nt) ydaN zinc transporter
?REL606 = 1460718 NA (NA)noncoding (559/768 nt) IS1 repeat region
* ? REL606 984122 =334 (1.060)4 (0.010) 4/130 22.1 1.4% coding (410/1749 nt) msbA fused lipid transporter subunits of ABC superfamily: membrane component/ATP‑binding component
?REL606 = 984118 282 (1.030)coding (406/1749 nt) msbA fused lipid transporter subunits of ABC superfamily: membrane component/ATP‑binding component
* ? REL606 3582149 =409 (1.290)4 (0.010) 4/138 22.7 1.1% coding (169/1224 nt) yhiS hypothetical protein
?REL606 3582330 = 324 (1.120)coding (350/1224 nt) yhiS hypothetical protein
* ? REL606 = 2424973297 (0.940)4 (0.010) 4/138 22.7 1.3% coding (1205/1695 nt) yfdU hypothetical protein
?REL606 = 2425480 318 (1.090)coding (698/1695 nt) yfdU hypothetical protein