breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 241 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | REL606 | 2,515,909 | 0 | A | C | 58.5% | 45.8 / 109.0 | 82 | V260G (GTG→GGG) | ypfI | predicted hydrolase |
* | REL606 | 720,466 | 0 | G | C | 57.8% | 0.6 / 60.9 | 45 | A23P (GCC→CCC) | phrB | deoxyribodipyrimidine photolyase, FAD‑binding |
* | REL606 | 1,854,401 | 0 | A | C | 48.2% | 97.9 / 65.3 | 112 | K88N (AAA→AAC) | yeaO | hypothetical protein |
* | REL606 | 1,747,903 | 0 | G | T | 48.2% | 372.3 / inf | 523 | A8S (GCA→TCA) | ydiL | hypothetical protein |
* | REL606 | 4,180,249 | 0 | T | G | 46.1% | 195.4 / 65.2 | 154 | G24G (GGT→GGG) | yjaH | hypothetical protein |
* | REL606 | 433 | 0 | T | G | 44.5% | 139.1 / 68.3 | 119 | V33G (GTG→GGG) | thrA | bifunctional aspartokinase I/homeserine dehydrogenase I |
* | REL606 | 2,376,348 | 0 | T | G | 44.5% | 248.3 / 65.2 | 191 | T63P (ACC→CCC) | folC | bifunctional folylpolyglutamate synthase/ dihydrofolate synthase |
* | REL606 | 4,392,556 | 0 | C | T | 44.3% | 189.3 / 176.1 | 228 | L43L (CTA→TTA) | aidB | isovaleryl CoA dehydrogenase |
* | REL606 | 3,774,914 | 0 | C | G | 39.5% | 316.2 / 187.3 | 215 | A20P (GCC→CCC) | yicJ | predicted transporter |
* | REL606 | 895,856 | 0 | G | C | 39.0% | 59.0 / 30.4 | 41 | A26P (GCC→CCC) | ECB_00836 | putative Regulatory protein |
* | REL606 | 4,429,282 | 0 | A | C | 38.6% | 241.7 / 11.9 | 129 | T472P (ACC→CCC) | ytfN | hypothetical protein |
* | REL606 | 4,111,429 | 0 | T | G | 37.7% | 190.6 / 21.0 | 106 | G496G (GGT→GGG) | metL | bifunctional aspartate kinase II/homoserine dehydrogenase II |
* | REL606 | 3,378,885 | 0 | T | G | 37.1% | 627.5 / 94.7 | 313 | T129P (ACC→CCC) | rplB | 50S ribosomal protein L2 |
* | REL606 | 1,655,458 | 0 | T | G | 35.8% | 567.0 / 176.8 | 321 | intergenic (‑360/‑164) | pntA/ydgH | NAD(P) transhydrogenase subunit alpha/hypothetical protein |
* | REL606 | 159,654 | 0 | A | C | 35.7% | 572.3 / 51.2 | 286 | G27G (GGT→GGG) | yadN | predicted fimbrial‑like adhesin protein |
* | REL606 | 931,314 | 0 | T | C | 34.8% | 182.8 / 51.7 | 139 | E84G (GAG→GGG) | ybjE | predicted transporter |
* | REL606 | 955,035 | 0 | A | C | 34.8% | 498.5 / 13.2 | 211 | T192P (ACC→CCC) | lolA | outer‑membrane lipoprotein carrier protein precursor |
* | REL606 | 2,441,705 | 0 | T | G | 34.5% | 216.1 / 12.1 | 114 | T139P (ACC→CCC) | ypdG | predicted enzyme IIC component of PTS |
* | REL606 | 1,118,209 | 0 | G | T | 34.5% | 68.0 / 47.0 | 58 | intergenic (‑444/‑311) | csgD/csgB | DNA‑binding transcriptional activator in two‑component regulatory system/curlin nucleator protein, minor subunit in curli complex |
* | REL606 | 3,230,856 | 0 | G | C | 34.4% | 87.9 / 28.4 | 61 | A77P (GCA→CCA) | yraN | hypothetical protein |
Marginal new junction evidence (lowest skew 10 of 70 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | = 3535515 | 0 (0.000) | 142 (0.470) +GGCT |
47/116 | 5.2 | 100% | coding (435/597 nt) | yhhF | predicted methyltransferase |
? | REL606 | 3535516 = | 0 (0.000) | coding (436/597 nt) | yhhF | predicted methyltransferase | |||||
* | ? | REL606 | = 3582336 | 356 (1.130) | 9 (0.030) | 7/140 | 20.7 | 2.8% | coding (356/1224 nt) | yhiS | hypothetical protein |
? | REL606 | = 3582485 | 289 (0.980) | coding (505/1224 nt) | yhiS | hypothetical protein | |||||
* | ? | REL606 | = 1506094 | 344 (1.090) | 10 (0.030) | 7/140 | 20.7 | 4.0% | coding (157/201 nt) | ECB_01417 | hypothetical protein |
? | REL606 | = 1506258 | 164 (0.560) | intergenic (+120/‑120) | ECB_01417/ydcC | hypothetical protein/hypothetical protein | |||||
* | ? | REL606 | 2041682 = | 268 (0.850) | 6 (0.020) | 5/128 | 21.2 | 2.2% | coding (1139/1428 nt) | wzx | O antigen translocase (Wzx) |
? | REL606 | 2041834 = | 294 (1.090) | coding (987/1428 nt) | wzx | O antigen translocase (Wzx) | |||||
* | ? | REL606 | 1405596 = | 236 (0.750) | 13 (0.040) | 6/140 | 21.4 | 5.6% | coding (377/984 nt) | ydaN | zinc transporter |
? | REL606 | 2034535 = | NA (NA) | noncoding (559/768 nt) | IS1 | repeat region | |||||
* | ? | REL606 | 2330426 = | 269 (0.850) | 7 (0.020) | 6/144 | 21.7 | 2.7% | coding (1201/1212 nt) | elaD | predicted enzyme |
? | REL606 | 2330659 = | 250 (0.820) | intergenic (+222/‑312) | elaD/yfbL | predicted enzyme/putative aminopeptidase; b2271_1 | |||||
* | ? | REL606 | 1405596 = | 236 (0.750) | 31 (0.110) | 5/140 | 22.1 | 12.3% | coding (377/984 nt) | ydaN | zinc transporter |
? | REL606 | = 1460718 | NA (NA) | noncoding (559/768 nt) | IS1 | repeat region | |||||
* | ? | REL606 | 984122 = | 334 (1.060) | 4 (0.010) | 4/130 | 22.1 | 1.4% | coding (410/1749 nt) | msbA | fused lipid transporter subunits of ABC superfamily: membrane component/ATP‑binding component |
? | REL606 | = 984118 | 282 (1.030) | coding (406/1749 nt) | msbA | fused lipid transporter subunits of ABC superfamily: membrane component/ATP‑binding component | |||||
* | ? | REL606 | 3582149 = | 409 (1.290) | 4 (0.010) | 4/138 | 22.7 | 1.1% | coding (169/1224 nt) | yhiS | hypothetical protein |
? | REL606 | 3582330 = | 324 (1.120) | coding (350/1224 nt) | yhiS | hypothetical protein | |||||
* | ? | REL606 | = 2424973 | 297 (0.940) | 4 (0.010) | 4/138 | 22.7 | 1.3% | coding (1205/1695 nt) | yfdU | hypothetical protein |
? | REL606 | = 2425480 | 318 (1.090) | coding (698/1695 nt) | yfdU | hypothetical protein |