Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A107 F1 I1 R1 19 326.9 20855348 93.1% 19416328 75.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 4,163,752 T→A I966N (ATC→AAC)  rpoB → DNA‑directed RNA polymerase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6064,163,7520TA97.1% 670.9 / NA 173I966N (ATC→AAC) rpoBDNA‑directed RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (116/50);  minor base C (0/5);  total (117/56)
Fisher's exact test for biased strand distribution p-value = 3.03e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 200 total aligned reads displayed.

ACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGA  >  REL606/4163680‑4163824
                                                                         |                                                                         
aCAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCt                                                                        <  2:3979565/76‑1 (MQ=255)
aCAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCt                                                                        <  2:5855343/76‑1 (MQ=255)
aCAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCt                                                                        <  2:7220685/76‑1 (MQ=255)
 cAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTc                                                                       >  1:9984663/1‑76 (MQ=255)
  aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCg                                                                      >  1:10049993/1‑76 (MQ=255)
  aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCg                                                                      >  1:6141924/1‑76 (MQ=255)
  aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCg                                                                      <  1:1598810/76‑1 (MQ=255)
   aaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAACAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGa                                                                     >  1:5463308/1‑76 (MQ=255)
    aCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGaa                                                                    <  1:3037766/76‑1 (MQ=255)
     cGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGACGAACTGCAGAACCTCGAAg                                                                   >  1:2043580/1‑76 (MQ=255)
     cGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAg                                                                   <  1:2265832/76‑1 (MQ=255)
     cGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAg                                                                   <  2:2081414/76‑1 (MQ=255)
     cGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAg                                                                   <  2:4624153/76‑1 (MQ=255)
      gTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGc                                                                  >  2:8553418/1‑76 (MQ=255)
      gTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGc                                                                  >  1:2069452/1‑76 (MQ=255)
         cgcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCggg                                                               <  1:9500459/76‑1 (MQ=255)
         cgcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCggg                                                               >  2:717136/1‑76 (MQ=255)
          gcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGt                                                              <  1:6532160/76‑1 (MQ=255)
            tGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCt                                                            >  1:1133023/1‑76 (MQ=255)
            tGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCt                                                            >  2:3589786/1‑76 (MQ=255)
             ggAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAAACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTg                                                           <  2:8012288/76‑1 (MQ=255)
               aaaTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGtt                                                         >  2:9242738/1‑76 (MQ=255)
                 aTCGACGAAATGCAGCGCACACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑Ca                                                      <  2:1809739/76‑1 (MQ=255)
                 aTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑Ca                                                      <  2:5345316/76‑1 (MQ=255)
                 aTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑Ca                                                      >  1:7998526/1‑76 (MQ=255)
                 aTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGGCTGTT‑Ca                                                      >  2:2847505/1‑76 (MQ=255)
                  tCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAg                                                     <  1:6776615/76‑1 (MQ=255)
                    gaagaaATGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGcc                                                   >  1:2723309/1‑76 (MQ=255)
                    gaagaaATGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGcc                                                   <  1:317342/76‑1 (MQ=255)
                     aagaaATGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCg                                                  <  2:10419799/76‑1 (MQ=255)
                     aagaaATGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGACg                                                  >  2:7785690/1‑76 (MQ=255)
                      agaaATGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGt                                                 >  2:8047747/1‑76 (MQ=255)
                       gaaATGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTa                                                <  1:9404127/76‑1 (MQ=255)
                        aaaTGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTAt                                               <  1:8656791/76‑1 (MQ=255)
                        aaaTGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTAt                                               <  1:9078868/76‑1 (MQ=255)
                        aaaTGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTAt                                               >  2:817931/1‑76 (MQ=255)
                        aaaTGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTAt                                               <  2:6749942/76‑1 (MQ=255)
                         aaTGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATc                                              >  1:6633979/1‑76 (MQ=255)
                         aaTGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCACGAAGCGGGTCTGTG‑CCGCCGTATc                                              >  1:7812661/1‑76 (MQ=255)
                          aTGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATcc                                             >  2:5560658/1‑76 (MQ=255)
                          aTGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATcc                                             <  1:5461020/76‑1 (MQ=255)
                             cAGCTAAAACAGGCGAAGAAAGACC‑TGTCGAAAGAACGGCAGAACCTAGAAGCGGGTCTTTC‑CAGCCGTATCCgtg                                          <  2:9116154/76‑1 (MQ=255)
                                 tCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgctgt                                      >  2:9529577/1‑76 (MQ=255)
                                 tCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgctgt                                      >  2:1162065/1‑76 (MQ=255)
                                 tCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgctgt                                      >  1:8799054/1‑76 (MQ=255)
                                 tCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgctgt                                      >  2:3877953/1‑76 (MQ=255)
                                 tCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgctgt                                      >  1:4321823/1‑76 (MQ=255)
                                  cAAACGGGCGAAGAAAGCCC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgctgtg                                     <  2:933924/76‑1 (MQ=255)
                                  cAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgctgtg                                     >  1:4388135/1‑76 (MQ=255)
                                  cAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgctgtg                                     >  1:6471094/1‑76 (MQ=255)
                                   aaaCAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgctgtgc                                    >  1:1079360/1‑76 (MQ=255)
                                      cgggCGAAGAAAGACC‑TGTCTGAAGAACTGCAGACCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTgg                                 <  1:3816962/74‑1 (MQ=255)
                                      cAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTgg                                 >  2:6880557/1‑76 (MQ=255)
                                       aGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGt                                >  2:8207269/1‑76 (MQ=255)
                                       aGGCGAAGAAAGACC‑TGTCTGAAGAACGGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGt                                <  2:6847673/76‑1 (MQ=255)
                                       aGGCGAAAAAAGACC‑TGTGTGAAGAACTGCAGAACCTCGAAGGGGGTCTTTT‑CAGCCGTATCCGTGCTGTGCTGGt                                <  2:3651400/76‑1 (MQ=255)
                                        ggCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTa                               <  1:10249125/76‑1 (MQ=255)
                                        ggCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTa                               <  1:5116178/76‑1 (MQ=255)
                                        atcGAGGAAAGCCC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTa                               <  1:5915687/74‑1 (MQ=255)
                                         gCGAAGAAAGCCC‑TGTCTAAAGACCTGCAAACCCCCGAAGCGGTTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAg                              <  2:2892504/76‑1 (MQ=255)
                                         gCGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAg                              >  2:7560523/1‑76 (MQ=255)
                                          caaagaaagcCC‑TGTCTGAAGAACTGCAGACCCCCGAAGCGGGTCTTTT‑CAGCCGTATCCGTGCTGTGCTGGTAGc                             <  2:6892693/74‑1 (MQ=255)
                                          cGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGc                             >  2:9544756/1‑76 (MQ=255)
                                          cGAAGAAAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGc                             >  1:194554/1‑76 (MQ=255)
                                           gaagaaAGACC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGcc                            >  2:6605600/1‑76 (MQ=255)
                                             agaaagaCC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCgg                          >  2:2394644/1‑76 (MQ=255)
                                             agaaagaCC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCgg                          >  2:2968688/1‑76 (MQ=255)
                                             aaaaagaCCTTGTCTGAAGAACTGCAAACCCTCGAGGGGGGTTTGTC‑CAGCCGTATCCGTGCTGTGCTGGTAGCCg                           <  2:4305326/74‑1 (MQ=255)
                                              gaaagaCC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGt                         >  1:3276964/1‑76 (MQ=255)
                                                 gaccc‑TGTCGGAACAACTGCAGACCCGCTAGGGGGTTCTGTC‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGc                      <  2:1738460/73‑1 (MQ=255)
                                                 agaCC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGc                      >  2:5448395/1‑76 (MQ=255)
                                                  gaCC‑TGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCg                     >  1:992489/1‑76 (MQ=255)
                                                       tGTCGGAAGACCGGCAGACCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGa                 <  1:1867335/76‑1 (MQ=255)
                                                        gtatGAAGAACGGCAGACCCTCAAGGCGTGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGTTAGCCGGTGGCGTTGaa                <  2:3377715/73‑1 (MQ=255)
                                                          cTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGc              >  1:745798/1‑76 (MQ=255)
                                                          cTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGc              >  1:1808411/1‑76 (MQ=255)
                                                          cTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGc              >  1:631623/1‑76 (MQ=255)
                                                          cTGAAGAACTGCAGAACCTCGAAGCGGGGCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGGGGCGGTGTAGc              >  2:2657850/1‑76 (MQ=255)
                                                           tGAAGAACTGCAGACCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCt             <  1:7755883/76‑1 (MQ=255)
                                                           tGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCt             >  2:381672/1‑76 (MQ=255)
                                                            gaagaaCTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTg            >  1:3554302/1‑76 (MQ=255)
                                                            gaagaaCTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTg            >  2:5128066/1‑76 (MQ=255)
                                                            gaaaaaCGGCAGACCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTg            <  1:410627/76‑1 (MQ=255)
                                                             aagacCCGCAGACCCGGGAGGGGGTTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTga           <  1:1198451/76‑1 (MQ=255)
                                                              agaaCTGCAGACCCTCGAAGCGGGTCGTTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTgag          <  1:4708374/76‑1 (MQ=255)
                                                                aaCTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGaa        >  1:7334918/1‑76 (MQ=255)
                                                                 aCTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAg       >  1:10401301/1‑76 (MQ=255)
                                                                 aCTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAg       >  2:1876300/1‑76 (MQ=255)
                                                                  cTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGc      >  1:9193935/1‑76 (MQ=255)
                                                                   tGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCt     >  1:2967289/1‑76 (MQ=255)
                                                                   tGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCt     >  1:6721768/1‑76 (MQ=255)
                                                                   tGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCt     >  1:3766995/1‑76 (MQ=255)
                                                                   tGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCt     >  2:5014260/1‑76 (MQ=255)
                                                                   gcgaGACCCTCG‑AGGGGGTTTGTTCCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCt     <  1:5513797/73‑1 (MQ=255)
                                                                    gCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTc    >  2:9772083/1‑76 (MQ=255)
                                                                     cAGACCCTCGAGGCGGGTCTGTC‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCg   <  1:7302771/76‑1 (MQ=255)
                                                                     cAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGACGCTGAGAAGCTCg   >  1:4513294/1‑76 (MQ=255)
                                                                     cAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCg   >  2:1639715/1‑76 (MQ=255)
                                                                      aGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGa  >  1:7009705/1‑76 (MQ=255)
                                                                      aGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGa  >  2:3507654/1‑76 (MQ=255)
                                                                         |                                                                         
ACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACC‑TGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGA  >  REL606/4163680‑4163824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A