Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A137 F1 I1 R1 18 221.5 13936938 95.6% 13323712 76.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 4,163,752 T→A I966N (ATC→AAC)  rpoB → DNA‑directed RNA polymerase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6064,163,7520TA99.3% 566.9 / NA 139I966N (ATC→AAC) rpoBDNA‑directed RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (99/39);  minor base C (0/1);  total (99/40)
Fisher's exact test for biased strand distribution p-value = 2.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 163 total aligned reads displayed.

ACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGA  >  REL606/4163680‑4163824
                                                                        |                                                                         
aCAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCt                                                                        <  1:737540/76‑1 (MQ=255)
aCAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCt                                                                        <  1:5859982/76‑1 (MQ=255)
aCAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCt                                                                        >  2:305912/1‑76 (MQ=255)
aCAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCt                                                                        >  2:467843/1‑76 (MQ=255)
aCAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCt                                                                        >  2:6209237/1‑76 (MQ=255)
 cAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTc                                                                       <  2:329268/76‑1 (MQ=255)
  aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCg                                                                      >  1:1738322/1‑76 (MQ=255)
  aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCg                                                                      >  2:5911852/1‑76 (MQ=255)
   aaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGa                                                                     <  2:4973054/76‑1 (MQ=255)
    aCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGaa                                                                    <  1:982675/76‑1 (MQ=255)
    aCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGaa                                                                    <  2:5002229/76‑1 (MQ=255)
      gTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGc                                                                  >  2:5719504/1‑76 (MQ=255)
       tGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCg                                                                 >  2:2029460/1‑76 (MQ=255)
       tGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCg                                                                 >  1:3068113/1‑76 (MQ=255)
         cgcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCggg                                                               >  2:1490539/1‑76 (MQ=255)
            tGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCt                                                            >  2:6126390/1‑76 (MQ=255)
            tGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCCCGAAGCGGGTCt                                                            <  1:5080661/76‑1 (MQ=255)
             ggAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTg                                                           >  1:4321064/1‑76 (MQ=255)
             ggAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTg                                                           <  1:4511025/76‑1 (MQ=255)
             ggAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTg                                                           <  2:6527501/76‑1 (MQ=255)
             ggAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTg                                                           >  1:161464/1‑76 (MQ=255)
             ggAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGACTg                                                           >  1:1363147/1‑76 (MQ=255)
               aaaTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGtt                                                         <  2:2030234/76‑1 (MQ=255)
                aaTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑c                                                       >  2:2163987/1‑76 (MQ=255)
                 aTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑Ca                                                      <  2:2465213/76‑1 (MQ=255)
                  tCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAg                                                     >  1:3998458/1‑76 (MQ=255)
                  tCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAg                                                     <  1:1775053/76‑1 (MQ=255)
                   cGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGc                                                    >  2:1808841/1‑76 (MQ=255)
                   cGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGc                                                    >  1:4459197/1‑76 (MQ=255)
                    gaagaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGcc                                                   >  1:1178633/1‑76 (MQ=255)
                    gaagaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGcc                                                   >  2:4338508/1‑76 (MQ=255)
                    gaagaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGcc                                                   >  2:468884/1‑76 (MQ=255)
                      agaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGt                                                 <  1:5223446/76‑1 (MQ=255)
                      agaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGt                                                 <  2:6706061/76‑1 (MQ=255)
                       gaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTa                                                >  1:1868137/1‑76 (MQ=255)
                        aaaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTAt                                               >  2:4507255/1‑76 (MQ=255)
                        aaaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTAt                                               <  2:2274776/76‑1 (MQ=255)
                        aaaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTAt                                               <  2:5143787/76‑1 (MQ=255)
                        aaaTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTAt                                               >  2:6474577/1‑76 (MQ=255)
                          aTGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATcc                                             <  2:3014997/76‑1 (MQ=255)
                           tGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCg                                            >  1:4158933/1‑76 (MQ=255)
                            gCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCCGTT‑CAGCCGTATACgt                                           >  1:1440477/1‑76 (MQ=255)
                              aGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgc                                         >  1:111657/1‑76 (MQ=255)
                               gCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgct                                        >  2:4810953/1‑76 (MQ=255)
                               gCTAAAACAGGCGAAGAAAGACCTGTCTAAAAACCTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgct                                        <  2:1808016/76‑1 (MQ=255)
                                  cAAACAGGCGAAGAAAGCCCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgctgtg                                     <  1:2042893/76‑1 (MQ=255)
                                  cAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgctgtg                                     >  2:874041/1‑76 (MQ=255)
                                   aaaCAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCgtgctgtgc                                    >  1:1771044/1‑76 (MQ=255)
                                     aCAGGCGAAGAAAGCCCTGTCTGAAGAAGTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGtgctgtgctg                                  <  2:4464277/76‑1 (MQ=255)
                                     aCAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGtgctgtgctg                                  <  2:3064051/76‑1 (MQ=255)
                                     aCAGGCGAAGAAAGACCTGTCTGAAGAACGGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCTGtgctgtgctg                                  >  2:6959408/1‑76 (MQ=255)
                                      cAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCCCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTgg                                 >  1:191988/1‑76 (MQ=255)
                                       aGGCGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGt                                >  2:2026799/1‑76 (MQ=255)
                                         gggaaaaaAGACCTTTCTGAAGAACTGCAGAACCTCAAAGCGGTTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAg                              <  2:1127364/74‑1 (MQ=255)
                                          cGAAGAAAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGc                             >  2:2271965/1‑76 (MQ=255)
                                           gaagaaAGACCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGcc                            >  2:5013960/1‑76 (MQ=255)
                                            aagaaagaCCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCg                           >  1:2157255/1‑76 (MQ=255)
                                            aaaaaagcCTTGTCGAAAGACCTGAAGACCCTCGAGGCGGTTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCg                           <  2:4654992/73‑1 (MQ=255)
                                             agaaagcCCTGTCTGAAGACCTGAAGACCCTGAAAGCGGGTCTTTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCgg                          <  2:3561820/76‑1 (MQ=255)
                                             aaaaagcCCGGTCTGAAGAACTGAAGACCCTCGAAGGGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCgg                          <  2:4875570/74‑1 (MQ=255)
                                               aaagcCCTGTCTGAAGACCTGCAGACCCTCGAAGGGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTg                        <  1:2450979/76‑1 (MQ=255)
                                               aaagaCCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCggcg                        >  2:4118648/1‑74 (MQ=255)
                                                aagcCCTGTCGGAAAACCTGCAAACCCGCGAGGGGGGTCTTTC‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTgg                       <  2:326341/76‑1 (MQ=255)
                                                aagcCCTGTCGAAAAAACTGCAGACCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTgg                       <  1:466110/76‑1 (MQ=255)
                                                aagaCCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTgg                       >  1:4623968/1‑76 (MQ=255)
                                                aagaCCTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTgg                       >  2:238467/1‑76 (MQ=255)
                                                  gcccTGTCGGAAAATCTGCAGACCCTCGACGGGGTTTTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCg                     <  1:6047796/74‑1 (MQ=255)
                                                     cTGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTg                  >  1:4256677/1‑76 (MQ=255)
                                                     cTGTCTGAAAACCGGCAGACCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTg                  <  1:5974215/76‑1 (MQ=255)
                                                      tGTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGa                 >  1:1694547/1‑76 (MQ=255)
                                                       gTCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGaa                >  2:1436886/1‑76 (MQ=255)
                                                        tCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAg               >  1:4296079/1‑76 (MQ=255)
                                                        tCTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAg               >  2:6793500/1‑76 (MQ=255)
                                                         cTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGc              >  2:72328/1‑76 (MQ=255)
                                                         cTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGc              >  1:3689041/1‑76 (MQ=255)
                                                         cTGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGc              >  1:2763545/1‑76 (MQ=255)
                                                          tGAAGAACTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCt             >  1:5361570/1‑76 (MQ=255)
                                                           gaagaaCTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTg            >  2:2805556/1‑76 (MQ=255)
                                                           gaagaaCTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTg            >  2:1717388/1‑76 (MQ=255)
                                                           aaagaaCTGCAGACCCTCGAGGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTg            <  1:3420257/75‑1 (MQ=255)
                                                            aagacCTGCAAACCCGCAAAGCGGGTCTTTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTga           <  1:2625113/76‑1 (MQ=255)
                                                            aagaaCTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTga           >  1:1328426/1‑76 (MQ=255)
                                                            aagaaCTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTga           >  1:2755612/1‑76 (MQ=255)
                                                            aagaaCTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGAGCTGGTAGCCGGTGGCGTTGGAGCTga           >  1:6440014/1‑76 (MQ=255)
                                                             agaaCTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTgag          >  2:4576038/1‑76 (MQ=255)
                                                               aaCTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTgagca        >  2:5986324/1‑74 (MQ=255)
                                                               aaCTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGaa        >  1:6542140/1‑76 (MQ=255)
                                                                aCTGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAg       >  2:3947561/1‑76 (MQ=255)
                                                                  tggaGAACCTCCAAGCGGGTTTTTTCCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGc      <  2:3766237/73‑1 (MQ=255)
                                                                  tGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCt     >  2:5520690/1‑76 (MQ=255)
                                                                  tGCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGGGGCGTTGCAGCTGAGAAGCt     >  2:2184749/1‑76 (MQ=255)
                                                                   gCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTc    >  1:6036279/1‑76 (MQ=255)
                                                                   gCAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTc    >  1:4651638/1‑76 (MQ=255)
                                                                    cAGACCCTGGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCg   <  1:3019571/76‑1 (MQ=255)
                                                                    cAGACCCCCGAGGCGGGTCTGTC‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCg   <  1:383458/76‑1 (MQ=255)
                                                                    cAGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCg   >  2:5461109/1‑76 (MQ=255)
                                                                     aGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGCGAAGCTCGa  >  1:3349874/1‑76 (MQ=255)
                                                                     aGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGa  >  1:5727695/1‑76 (MQ=255)
                                                                     aGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGa  >  1:5736124/1‑76 (MQ=255)
                                                                     aGAACCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGa  >  2:2911668/1‑76 (MQ=255)
                                                                        |                                                                         
ACAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTT‑CAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCTCGA  >  REL606/4163680‑4163824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A