Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A139 F1 I1 R1
|
15 |
48.9 |
3081653 |
97.3% |
2998448 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,193,694 |
G→T |
intergenic (‑126/‑31) |
yjaB ← / → metA |
predicted acetyltransferase/homoserine O‑succinyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,193,694 | 0 | G | T | 90.6%
| 158.3
/ 14.1
| 53 | intergenic (‑126/‑31) | yjaB/metA | predicted acetyltransferase/homoserine O‑succinyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (5/0); new base T (27/21); total (32/21) |
| Fisher's exact test for biased strand distribution p-value = 1.44e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.13e-01 |
CTTTCTCACCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGATGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGCTACCCGCCGTCAATTTC > REL606/4193620‑4193766
|
cTTTCTCACCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGATGTCTAAACGt > 2:888104/1‑76 (MQ=255)
tctcACCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGATGTCTAAACTtata < 1:1462358/76‑1 (MQ=255)
tctcACCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGATGTCTAAACGtata > 1:47865/1‑76 (MQ=255)
ctcACCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGATGTCTAAACTTATaa > 1:280357/1‑76 (MQ=255)
cACCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGATGTCTAAACTTATAAGc > 2:1432155/1‑76 (MQ=255)
cACCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGATGTCTAAACTTATAAGc > 2:1083884/1‑76 (MQ=255)
cACCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGATGTCTAAACTTATAAGc > 2:1022299/1‑76 (MQ=255)
aCCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGATGTCTAAACTTATAAGCg > 2:76234/1‑76 (MQ=255)
ttCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGATGTCTAAACTTATAAGCGTAt > 2:1155582/1‑76 (MQ=255)
tCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGATCTCTAAACTTATAAGCGTATg < 2:732166/76‑1 (MQ=255)
cAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGATGTCTAAACTTATAAGCGTATGt < 2:298907/76‑1 (MQ=255)
cATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGATGTCTAAACTTATAAGCGTATGTAGt > 2:1100684/1‑76 (MQ=255)
cATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGATGTCTAAACTTATAAGCGTATGTAGt < 2:25126/76‑1 (MQ=255)
tGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCATCTATCTGGATGTCTAAACTTATAAGCGTATGTAGTGa < 2:756338/76‑1 (MQ=255)
cAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGATGTCTAAACTTATAAGCGTATGTAGTGAgg < 1:1501936/76‑1 (MQ=255)
aGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGATGTCTAAACTTATAAGCGTATGTAGTGAGGt < 1:498800/76‑1 (MQ=255)
ggCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGATGTCTAAACTTATAAGCGTATGTAGTGAGGTa < 1:963661/76‑1 (MQ=255)
gCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGATGTCTAAACTTATAAGCGTATGTAGTGAGGTaa > 2:426750/1‑76 (MQ=255)
gCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGATGTCTAAACTTATAAGCGTATGTAGTGAGGTaa > 1:942419/1‑76 (MQ=255)
tCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGATGTCTAAACTTATAAGCGTATGTAGTGAGGTAATc < 2:311745/76‑1 (MQ=255)
tCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGATGTCTAAACTTATAAGCGTATGTAGTGAGGTAATc < 1:1236912/76‑1 (MQ=255)
cGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGATGTCTAAACTTATAAGCGTATGTAGTGAGGTAATCa > 1:843394/1‑76 (MQ=255)
cGACATTGGCAAATTTTCTGGTTATCTTCAGCTATATGGATGTCTAAACTTATAAGCGTATGTAGTGAGGTAATCa > 2:1364330/1‑76 (MQ=255)
gACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGATGTCTAAACTTATAAGCGTATGTAGTGAGGTAATCAg > 1:1453754/1‑76 (MQ=255)
aTTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGATGTCTAAACTTATAAGCGTATGTAGTGAGGTAATCAGGtt > 2:650069/1‑76 (MQ=255)
ggCAAATTTTCTGGTTATCTTCAGCTATCTGGATGTCTAAACTTATAAGCGTATGTAGTGAGGTAATCAGGTTATg < 2:1221336/76‑1 (MQ=255)
aaaTTTTCTGGTTATCTTCAGCTATCTGGATGTCTAAACTTATAAGCGTATGTAGTGAGGTAATCAGGCTATGCCg > 2:1437665/1‑76 (MQ=255)
aTTTTCTGGTTATCTTCAGCTATCTGGATGTCTAAACTTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGAt > 2:473753/1‑76 (MQ=255)
ntttCTGGTTATCTTCAGCTATCTGGATGTCTAAACTTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGAtt > 1:1210893/2‑76 (MQ=255)
tCTGGTTATCTTCAGCTATCTGGATGTCTAAACTTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCgt > 1:1146820/1‑76 (MQ=255)
tGGTTATCTTCAGCTATCTGGATGTCTAAACTTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCgtgt > 2:1233992/1‑76 (MQ=255)
gTTATCTTCAGCTATCTGGATGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGc > 1:424302/1‑76 (MQ=255)
ttATCTTTTGCTATCTGGATGTCTAAACTTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGcc > 2:999778/1‑76 (MQ=255)
ttATCTTCAGCTATCTGGATGTCTAAACTTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGcc > 2:1508355/1‑76 (MQ=255)
tCTTCAGCTATCTGGATGTCTAAACTTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGa < 1:1117915/76‑1 (MQ=255)
ttCAGGTATCTGGATGTCTAAACTTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACg > 2:1298270/1‑76 (MQ=255)
cAGCTATCTGGATGTCTAAACTTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAg < 2:1146819/76‑1 (MQ=255)
cAGCTATCTGGATGTCTAAACTTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAg < 2:1462404/76‑1 (MQ=255)
gCTATCTGGATGTCTAAACTTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGCt < 2:1346062/76‑1 (MQ=255)
gCTATCTGGATGTCTAAACTTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGCt < 2:612434/76‑1 (MQ=255)
cTATCTGGATGTCTAAACTTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGCTa < 1:673963/76‑1 (MQ=255)
cTATCTGGATGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGCTa > 1:1380850/1‑76 (MQ=255)
tATCTGGATGTCTAAACTTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGCTAc > 1:1077242/1‑76 (MQ=255)
tATCTGGATGTCTAAACTTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGCTAc < 2:1331234/76‑1 (MQ=255)
tATCTGGATGTCTAAACTTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGCTAc < 2:429684/76‑1 (MQ=255)
cTGGATGTCTAAACTTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGGGCTACccg < 1:670997/76‑1 (MQ=255)
cTGGATGTCTAAACTTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGCTACccg > 1:893433/1‑76 (MQ=255)
tGGATGTCTAAACTTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGCTACccgc < 2:1127461/76‑1 (MQ=255)
ggATGTCTAAACTTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGCTACccgcc > 1:904775/1‑76 (MQ=255)
ggATGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGCTACccgcc > 1:367320/1‑76 (MQ=255)
tCTAAACTTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACAAGCTACCCGCCGTCaa > 1:210403/1‑76 (MQ=255)
aaaCTTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGCTACCCGCCGTCAAttt > 1:347263/1‑76 (MQ=255)
aaCTTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGCTACCCGCCGTCAATTTc > 1:1506500/1‑76 (MQ=255)
|
CTTTCTCACCTTCAACATGCAGGCTCGACATTGGCAAATTTTCTGGTTATCTTCAGCTATCTGGATGTCTAAACGTATAAGCGTATGTAGTGAGGTAATCAGGTTATGCCGATTCGTGTGCCGGACGAGCTACCCGCCGTCAATTTC > REL606/4193620‑4193766
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
