breseq  version 0.33.1  revision 8505477f25b3 mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence	position	mutation	annotation	gene	description
RA	433,653	T→G	F50V (TTT→GTT)	hupB →	HU, DNA‑binding transcriptional regulator, beta subunit
RA	1,081,210	(T)8→7	coding (885/1260 nt)	yccE →	hypothetical protein
RA	1,126,975	T→C	L515P (CTG→CCG)	mdoH →	glucosyltransferase MdoH
RA	1,329,516	C→T	H33Y (CAC→TAC)	topA →	DNA topoisomerase I
RA	1,689,712	(C)6→7	intergenic (+360/‑251)	nth → / → ydgR	DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)/putative tripeptide transporter permease
JC	1,733,297	(CGTATGAA)1→2	coding (333/1413 nt)	pykF →	pyruvate kinase
JC JC	1,886,669	IS1 (–) +9 bp	intergenic (‑499/+162)	yobF ← / ← yebO	hypothetical protein/hypothetical protein
RA	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein
RA	3,762,741	A→T	K662I (AAA→ATA)	spoT →	bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA	3,875,632	(T)7→8	intergenic (‑66/+287)	glmU ← / ← atpC	bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
MC JC	3,894,997	Δ6,934 bp	IS150‑mediated	rbsD–[yieO]	rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
RA	4,162,470	A→C	T539P (ACC→CCC)	rpoB →	DNA‑directed RNA polymerase subunit beta
RA	4,163,752	T→G	I966S (ATC→AGC)	rpoB →	DNA‑directed RNA polymerase subunit beta
RA	4,193,694	G→T	intergenic (‑126/‑31)	yjaB ← / → metA	predicted acetyltransferase/homoserine O‑succinyltransferase
JC JC	4,524,522	IS186 (+) +6 bp	coding (494‑499/549 nt)	fimA →	major type 1 subunit fimbrin (pilin)

Unassigned missing coverage evidence
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	REL606	1607980–1609176	1609746	571–1767	14 [12]	[12] 13	[insE‑3]–ybcW	[insE‑3],insF‑3,ybcW
*	*	÷	REL606	1610064	1614190	4127	13 [12]	[12] 14	ynfN–[ECB_01522]	ynfN,ECB_01516,cspI,ydfP,ydfQ,ydfR,essQ,[ECB_01522]
*	*	÷	REL606	1614469	1616663–1615471	1003–2195	13 [12]	[12] 13	ECB_01523–[insF‑4]	ECB_01523,insE‑4,[insF‑4]
*	*	÷	REL606	2031582–2031671	2032989	1319–1408	16 [12]	[12] 13	[manB]–[manC]	[manB],[manC]
*	*	÷	REL606	2035963	2038569	2607	13 [12]	[12] 13	[wbbC]–[wbbB]	[wbbC],wzy,[wbbB]
*	*	÷	REL606	2038575	2039520	946	13 [12]	[12] 13	[wbbB]–[wbbA]	[wbbB],[wbbA]
*	*	÷	REL606	2039819	2041437	1619	13 [12]	[12] 13	[vioB]–[wzx]	[vioB],vioA,[wzx]
*	*	÷	REL606	2042975	2052771	9797	13 [12]	[12] 13	[rmlC]–[wzxC]	[rmlC],rfbA,rfbD,rfbB,galF,wcaM,wcaL,wcaK,[wzxC]
*	*	÷	REL606	2052777	2054858–2054826	2050–2082	13 [12]	[12] 14	[wzxC]–[cpsG]	[wzxC],wcaJ,[cpsG]