Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A94 F1 I1 R1
|
15 |
48.6 |
3202402 |
95.4% |
3055091 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,251,818 |
C→T |
A315T (GCG→ACG) |
nusA ← |
transcription elongation factor NusA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,251,818 | 0 | C | T | 91.2%
| 109.0
/ ‑0.5
| 34 | A315T (GCG→ACG) | nusA | transcription elongation factor NusA |
| Reads supporting (aligned to +/- strand): ref base C (2/1); new base T (11/20); total (13/21) |
| Fisher's exact test for biased strand distribution p-value = 5.44e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTG > REL606/3251750‑3251889
|
gTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTa > 1:940669/1‑76 (MQ=255)
gTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTa < 1:295215/76‑1 (MQ=255)
gAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCg > 1:1576537/1‑76 (MQ=255)
tCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCtt < 1:1426896/76‑1 (MQ=255)
cccAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCCTc > 1:180802/1‑76 (MQ=255)
ccAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCa < 2:1273813/76‑1 (MQ=255)
ccAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCa < 1:246326/76‑1 (MQ=255)
ccAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCa < 1:526906/76‑1 (MQ=255)
gCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACNGGCTTCAACggag < 1:1078472/76‑3 (MQ=255)
gCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCg < 1:813284/76‑1 (MQ=255)
cTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCCTCAACGGCGa > 2:833483/1‑76 (MQ=255)
tCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGat < 1:1046418/76‑1 (MQ=255)
aGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGGGCCAGGCTACCGGCTTCAACGGGGatat > 1:1517224/1‑76 (MQ=255)
cTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATcc < 1:422724/76‑1 (MQ=255)
aaGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTTCACGGCGATATCCATggg > 2:261229/1‑75 (MQ=255)
aaGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGgt < 2:986768/76‑1 (MQ=255)
cGCACGTCCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTAt < 2:421680/76‑1 (MQ=255)
gCACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATc > 2:916356/1‑76 (MQ=255)
cACGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTTAACGGCGAGATCCATGGGGGGGTTATCt > 2:544220/1‑76 (MQ=255)
aCGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCtt < 1:1121940/76‑1 (MQ=255)
aCGTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCtt > 1:630190/1‑76 (MQ=255)
gTTCTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCa < 1:1426620/76‑1 (MQ=255)
gTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCCACGGCGGTATCCCTGGTGTGTTTATCttta > 2:360106/1‑74 (MQ=255)
cTGACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATcc < 2:1465413/76‑1 (MQ=255)
gACCGTTACGGCCAGTCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATccac > 1:863436/1‑76 (MQ=255)
gACCGTTACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATccac > 2:752563/1‑76 (MQ=255)
tACGGCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGAt < 1:1015642/76‑1 (MQ=255)
ggCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGa < 1:57217/76‑1 (MQ=255)
gCCAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGaa < 1:1590854/76‑1 (MQ=255)
ccAATCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAg < 2:764649/76‑1 (MQ=255)
aaTCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCa > 1:749179/1‑76 (MQ=255)
aaTCGCCTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCa > 1:596307/1‑76 (MQ=255)
ccTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTc < 1:341910/76‑1 (MQ=255)
cTGCGTCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCt < 1:255291/76‑1 (MQ=255)
tGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTg < 1:1555623/76‑1 (MQ=255)
cgcgtCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTg < 2:416072/75‑1 (MQ=255)
|
GTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTG > REL606/3251750‑3251889
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
