breseq  version 0.33.1  revision 8505477f25b3 mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence	position	mutation	annotation	gene	description
RA	649,601	T→C	I401V (ATC→GTC)	mrdA ←	transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)
RA	980,321	G→A	G412D (GGC→GAC)	rpsA →	30S ribosomal protein S1
RA	1,329,516	C→T	H33Y (CAC→TAC)	topA →	DNA topoisomerase I
JC	1,733,297	(CGTATGAA)1→2	coding (333/1413 nt)	pykF →	pyruvate kinase
RA	2,032,114	A→G	R228R (CGT→CGC)	manB ←	Phosphomannomutase
RA	2,131,466	(A)9→8	intergenic (‑125/+175)	gatY ← / ← fbaB	D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase
RA	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein
RA	3,251,818	C→T	A315T (GCG→ACG)	nusA ←	transcription elongation factor NusA
RA	3,414,644	A→G	Y207C (TAC→TGC)	crp →	DNA‑binding transcriptional dual regulator
RA	3,497,251	G→A	S262F (TCC→TTC)	glgP ←	glycogen phosphorylase
RA	3,762,741	A→T	K662I (AAA→ATA)	spoT →	bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA	3,875,632	(T)7→8	intergenic (‑66/+287)	glmU ← / ← atpC	bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
MC JC	3,894,997	Δ6,934 bp	IS150‑mediated	rbsD–[yieO]	rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
RA	4,161,106	A→G	E84G (GAA→GGA)	rpoB →	DNA‑directed RNA polymerase subunit beta
JC JC	4,524,522	IS186 (+) +6 bp	coding (494‑499/549 nt)	fimA →	major type 1 subunit fimbrin (pilin)

Unassigned missing coverage evidence
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	REL606	761782–761800	762407–762361	562–626	12 [11]	[9] 12	[lysW]–[lysQ]	[lysW],valZ,lysY,lysZ,[lysQ]
*	*	÷	REL606	2032123	2033331	1209	14 [11]	[11] 12	[manB]–[manC]	[manB],[manC]
*	*	÷	REL606	2043389	2055391	12003	12 [11]	[11] 12	[rfbA]–[cpsG]	[rfbA],rfbD,rfbB,galF,wcaM,wcaL,wcaK,wzxC,wcaJ,[cpsG]