Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A77 F1 I1 R1
|
21 |
53.0 |
3608474 |
95.6% |
3449701 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,141,329 |
Δ1 bp |
intergenic (‑84/+110) |
ECB_02035 ← / ← ECB_02036 |
hypothetical protein/hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,141,329 | 0 | A | . | 96.3%
| 246.9
/ 3.7
| 54 | intergenic (‑84/+110) | ECB_02035/ECB_02036 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base . (19/33); total (20/34) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01 |
TCTTTGCTAAGGATCGCTGTTATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTGATCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCTTATCCTTCGTGAGATTATTT > REL606/2141254‑2141396
|
tctTTGCTAAGGATCGCTGTTATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTGa > 2:10112/1‑76 (MQ=255)
tGCTAAGGATCGCTGTTATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAAc > 1:1500255/1‑76 (MQ=255)
gCTAAGGATCGCTGTTATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACg > 1:1783168/1‑76 (MQ=255)
gCTAAGGATCGCTGTTATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACg < 1:1098212/76‑1 (MQ=255)
cTAAGGATCGCTGTTATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTGATCAACg > 2:1650530/1‑76 (MQ=255)
aaGGATCGCTGTTATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCt > 2:972471/1‑76 (MQ=255)
aaGGATCGCTGTTATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCt > 2:197825/1‑76 (MQ=255)
aaGGATCGCTGTTATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCt > 2:215454/1‑76 (MQ=255)
aGGATCGCTGTTATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTc > 2:263224/1‑76 (MQ=255)
cGCTGTTATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAg < 1:1376000/76‑1 (MQ=255)
gCTGTTATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGt < 1:1192622/76‑1 (MQ=255)
tGTTATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTAt < 1:453818/76‑1 (MQ=255)
gTTATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATc > 1:1003255/1‑76 (MQ=255)
gTTATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATc > 2:1761366/1‑76 (MQ=255)
ttATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTACGTATCt > 2:572193/1‑76 (MQ=255)
ttATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCt < 1:1432634/76‑1 (MQ=255)
tATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCtg < 2:294674/76‑1 (MQ=255)
aTGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCtgt < 1:1107122/76‑1 (MQ=255)
tGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCtgtt < 1:1297039/76‑1 (MQ=255)
tGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCtgtt < 2:780011/76‑1 (MQ=255)
aGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCtgttgt > 1:482302/1‑76 (MQ=255)
aGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCtgttgt > 2:993774/1‑76 (MQ=255)
gTTCCGATGCCAGAGCGGCAGCGGGTTTATCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTtgtg < 2:1261937/76‑1 (MQ=255)
gTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTtgtg < 2:275056/76‑1 (MQ=255)
gTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTtgtg < 2:1538529/76‑1 (MQ=255)
gTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTtgtg < 2:1261926/76‑1 (MQ=255)
ttCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTGATCAACGCCTCGTAAGTATCTGTtgtg < 1:10112/76‑1 (MQ=255)
tCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGaa < 1:812299/76‑1 (MQ=255)
tCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGaa < 1:65839/76‑1 (MQ=255)
tCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGaa > 1:722502/1‑76 (MQ=255)
ccGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGaaa < 1:1184320/76‑1 (MQ=255)
ccGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGaaa < 1:1184326/76‑1 (MQ=255)
gATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAAtt < 2:1442560/76‑1 (MQ=255)
cAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGAttt < 1:496433/76‑1 (MQ=255)
cAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGAttt < 2:1032629/76‑1 (MQ=255)
cAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGAttt < 2:755841/76‑1 (MQ=255)
agagCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGAtttt < 1:1450833/76‑1 (MQ=255)
agagCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGAtttt < 2:283270/76‑1 (MQ=255)
agagCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGAtttt < 2:1671282/76‑1 (MQ=255)
agCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCa < 2:314630/76‑1 (MQ=255)
cGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATc > 2:1072221/1‑76 (MQ=255)
gggTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCtt > 1:554677/1‑76 (MQ=255)
gggTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCtt < 1:509468/76‑1 (MQ=255)
ggTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCTTa < 2:556302/76‑1 (MQ=255)
gTTTGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCTTAt > 1:841244/1‑76 (MQ=255)
tGTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCTTATCCt > 1:703315/1‑76 (MQ=255)
gTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCTTATCCtt < 2:1094176/76‑1 (MQ=255)
gTCTTCTTTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCTTATCCtt > 1:1060515/1‑76 (MQ=255)
cttcttTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCTTATCCTTCg > 1:803125/1‑76 (MQ=255)
cttcttTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCTTATCCTTCg < 1:337700/76‑1 (MQ=255)
cttcttTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCTTATCCTTCg < 1:337693/76‑1 (MQ=255)
tcttTGTTGGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCTTATCCTTCGTg > 1:948742/1‑76 (MQ=255)
gttgGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCTTATCCTTCGTGAGatt > 1:625819/1‑76 (MQ=255)
ttgGCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCTTATCCTTCGTGAGatta > 2:1555994/1‑76 (MQ=255)
gCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCTTATCCTTCGTGAGAttattt < 2:1431788/76‑1 (MQ=255)
gCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCTTATCCTTCGTGAGAttattt < 2:97845/76‑1 (MQ=255)
gCACGGTTG‑TCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCTTATCCTTCGTGAGAttattt < 1:981301/76‑1 (MQ=255)
|
TCTTTGCTAAGGATCGCTGTTATGAGTTCCGATACCAGAGCGGCAGCGGGTTTGTCTTCTTTGTTGGCACGGTTGATCAACGCCTCGTAAGTATCTGTTGTGAAATTGATTTTCATCGTGTCTTATCCTTCGTGAGATTATTT > REL606/2141254‑2141396
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
