Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A31 F999 I0 R1 22 6.7 608417 40.4% 245800 128.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,604,716 C→A A186E (GCA→GAA)  bglJ → DNA‑binding transcriptional regulator BglJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,7160CA100.0% 12.1 / NA 7A186E (GCA→GAA) bglJDNA‑binding transcriptional regulator BglJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (3/4);  total (3/4)

ATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGC  >  NC_000913/4604649‑4604826
                                                                   |                                                                                                              
aTGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCCATATCAAAACGATCCGTGAACATAAATTTAAAGTATGGTCGAAACTGGGCGTCCATTCTCAC‑CGGGGTTGTTTGGGGccc                                                  >  1:80124/1‑128 (MQ=255)
                    gACACAAATTGCCGGGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTtatgtat                               <  1:304201/129‑1 (MQ=255)
                          aaTTGCCGAACA‑CTTAAAACCAAATACAAAACGGTCCCTGAACATAAATTTAATGGGAGTTCGAAACTGGGCGGCATTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCAt                        <  1:472216/129‑1 (MQ=255)
                           aTTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGCCAGTTCTACCCCAGGGTGTTTGGGGCCCCCAGAT‑TTCCGTTTTTTATGGGGCAt                        >  1:213255/1‑128 (MQ=255)
                            ttGCCGGGCAGCTTAAACGCAAAATCAAAACGATCCGTGAACATAAATTTAATGGGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCAtt                       <  1:490243/129‑1 (MQ=255)
                                                aaTATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTTGTTATGCGGCATTGCGAAACAAGAAATGTGttc   >  1:279198/1‑128 (MQ=255)
                                                 aaaacAAAACGGTCCGTGAACATAAATTTAATGGGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGc  <  1:330908/125‑1 (MQ=255)
                                                                   |                                                                                                              
ATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGC  >  NC_000913/4604649‑4604826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A