Genome-Reduced Ecoli: application_of_ALE_to_re-optimize_growth_performance_of_genome-reduced_strain_MS56-M9-MG1655gbk - Sample Meta Data
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Note: the reference genome BOP27 is a known variant of E. coli K12 MG1655 E. coli K12 MG1655.
| ALE, Flask, Isolate, Technical Replicate | Clonal or Population | ALE Sample Details | Base Media | Carbon Source(g/L) | Nitrogen Source(g/L) | Phosphorus Source(g/L) | Sulfur Source(g/L) | Calcium Source(g/L) | Supplement(g/L) | Temperature (Celsius) | Strain | Additional Strain Details | Taxonomy ID | Reference Genome |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| A1 F999 I0 R1 | clonal | initially supplemented with 0.1% LB medium reduced in a stepwise manner to eventually achieve supplement-free growth. | M9 | glucose(2g/L) | NH4Cl(1g/L) | KH2PO4(3g/L),Na2HPO4(6.78g/L) | MgSO4(1mM) | CaCl2(0.1mM) | 37 | 511145 | Escherichia coli MS56 | Illumina/ 50 | /var/data/MG1655.gbk | |
| A2 F999 I0 R1 | clonal | initially supplemented with 0.1% LB medium reduced in a stepwise manner to eventually achieve supplement-free growth. | M9 | glucose(2g/L) | NH4Cl(1g/L) | KH2PO4(3g/L),Na2HPO4(6.78g/L) | MgSO4(1mM) | CaCl2(0.1mM) | 37 | 511145 | Escherichia coli MS56 | Illumina/ 50 | /var/data/MG1655.gbk | |
| A3 F999 I0 R1 | clonal | initially supplemented with 0.1% LB medium reduced in a stepwise manner to eventually achieve supplement-free growth. | M9 | glucose(2g/L) | NH4Cl(1g/L) | KH2PO4(3g/L),Na2HPO4(6.78g/L) | MgSO4(1mM) | CaCl2(0.1mM) | 37 | 511145 | Escherichia coli MS56 | Illumina/ 50 | /var/data/MG1655.gbk | |
| A4 F999 I0 R1 | clonal | initially supplemented with 0.1% LB medium reduced in a stepwise manner to eventually achieve supplement-free growth. | M9 | glucose(2g/L) | NH4Cl(1g/L) | KH2PO4(3g/L),Na2HPO4(6.78g/L) | MgSO4(1mM) | CaCl2(0.1mM) | 37 | 511145 | Escherichia coli MS56 | Illumina/ 50 | /var/data/MG1655.gbk | |
| A5 F999 I0 R1 | clonal | initially supplemented with 0.1% LB medium reduced in a stepwise manner to eventually achieve supplement-free growth. | M9 | glucose(2g/L) | NH4Cl(1g/L) | KH2PO4(3g/L),Na2HPO4(6.78g/L) | MgSO4(1mM) | CaCl2(0.1mM) | 37 | 511145 | Escherichia coli MS56 | Illumina/ 50 | /var/data/MG1655.gbk | |
| A6 F999 I0 R1 | clonal | initially supplemented with 0.1% LB medium reduced in a stepwise manner to eventually achieve supplement-free growth. | M9 | glucose(2g/L) | NH4Cl(1g/L) | KH2PO4(3g/L),Na2HPO4(6.78g/L) | MgSO4(1mM) | CaCl2(0.1mM) | 37 | 511145 | Escherichia coli MS56 | Illumina/ 50 | /var/data/MG1655.gbk | |
| A7 F999 I0 R1 | clonal | initially supplemented with 0.1% LB medium reduced in a stepwise manner to eventually achieve supplement-free growth. | M9 | glucose(2g/L) | NH4Cl(1g/L) | KH2PO4(3g/L),Na2HPO4(6.78g/L) | MgSO4(1mM) | CaCl2(0.1mM) | 37 | 511145 | Escherichia coli MS56 | Illumina/ 50 | /var/data/MG1655.gbk | |
| A8 F999 I0 R1 | clonal | initially supplemented with 0.1% LB medium reduced in a stepwise manner to eventually achieve supplement-free growth. | M9 | glucose(2g/L) | NH4Cl(1g/L) | KH2PO4(3g/L),Na2HPO4(6.78g/L) | MgSO4(1mM) | CaCl2(0.1mM) | 37 | 511145 | Escherichia coli MS56 | Illumina/ 50 | /var/data/MG1655.gbk | |
| A9 F999 I0 R1 | clonal | initially supplemented with 0.1% LB medium reduced in a stepwise manner to eventually achieve supplement-free growth. | M9 | glucose(2g/L) | NH4Cl(1g/L) | KH2PO4(3g/L),Na2HPO4(6.78g/L) | MgSO4(1mM) | CaCl2(0.1mM) | 37 | 511145 | Escherichia coli MS56 | Illumina/ 50 | /var/data/MG1655.gbk | |
| A10 F999 I0 R1 | clonal | initially supplemented with 0.1% LB medium reduced in a stepwise manner to eventually achieve supplement-free growth. | M9 | glucose(2g/L) | NH4Cl(1g/L) | KH2PO4(3g/L),Na2HPO4(6.78g/L) | MgSO4(1mM) | CaCl2(0.1mM) | 37 | 511145 | Escherichia coli MS56 | Illumina/ 50 | /var/data/MG1655.gbk | |
| A11 F999 I0 R1 | clonal | initially supplemented with 0.1% LB medium reduced in a stepwise manner to eventually achieve supplement-free growth. | M9 | glucose(2g/L) | NH4Cl(1g/L) | KH2PO4(3g/L),Na2HPO4(6.78g/L) | MgSO4(1mM) | CaCl2(0.1mM) | 37 | 511145 | Escherichia coli MS56 | Illumina/ 50 | /var/data/MG1655.gbk | |
| A12 F999 I0 R1 | clonal | initially supplemented with 0.1% LB medium reduced in a stepwise manner to eventually achieve supplement-free growth. | M9 | glucose(2g/L) | NH4Cl(1g/L) | KH2PO4(3g/L),Na2HPO4(6.78g/L) | MgSO4(1mM) | CaCl2(0.1mM) | 37 | 511145 | Escherichia coli MS56 | Illumina/ 50 | /var/data/MG1655.gbk | |
| A13 F999 I0 R1 | clonal | initially supplemented with 0.1% LB medium reduced in a stepwise manner to eventually achieve supplement-free growth. | M9 | glucose(2g/L) | NH4Cl(1g/L) | KH2PO4(3g/L),Na2HPO4(6.78g/L) | MgSO4(1mM) | CaCl2(0.1mM) | 37 | 511145 | Escherichia coli MS56 | Illumina/ 50 | /var/data/MG1655.gbk | |
| A14 F999 I0 R1 | clonal | initially supplemented with 0.1% LB medium reduced in a stepwise manner to eventually achieve supplement-free growth. | M9 | glucose(2g/L) | NH4Cl(1g/L) | KH2PO4(3g/L),Na2HPO4(6.78g/L) | MgSO4(1mM) | CaCl2(0.1mM) | 37 | 511145 | Escherichia coli MS56 | Illumina/ 50 | /var/data/MG1655.gbk | |
| A15 F999 I0 R1 | clonal | initially supplemented with 0.1% LB medium reduced in a stepwise manner to eventually achieve supplement-free growth. | M9 | glucose(2g/L) | NH4Cl(1g/L) | KH2PO4(3g/L),Na2HPO4(6.78g/L) | MgSO4(1mM) | CaCl2(0.1mM) | 37 | 511145 | Escherichia coli MS56 | Illumina/ 50 | /var/data/MG1655.gbk | |
| A16 F999 I0 R1 | clonal | initially supplemented with 0.1% LB medium reduced in a stepwise manner to eventually achieve supplement-free growth. | M9 | glucose(2g/L) | NH4Cl(1g/L) | KH2PO4(3g/L),Na2HPO4(6.78g/L) | MgSO4(1mM) | CaCl2(0.1mM) | 37 | 511145 | Escherichia coli MS56 | Illumina/ 50 | /var/data/MG1655.gbk | |
| A17 F999 I0 R1 | clonal | initially supplemented with 0.1% LB medium reduced in a stepwise manner to eventually achieve supplement-free growth. | M9 | glucose(2g/L) | NH4Cl(1g/L) | KH2PO4(3g/L),Na2HPO4(6.78g/L) | MgSO4(1mM) | CaCl2(0.1mM) | 37 | 511145 | Escherichia coli MS56 | Illumina/ 50 | /var/data/MG1655.gbk | |
| A18 F999 I0 R1 | clonal | initially supplemented with 0.1% LB medium reduced in a stepwise manner to eventually achieve supplement-free growth. | M9 | glucose(2g/L) | NH4Cl(1g/L) | KH2PO4(3g/L),Na2HPO4(6.78g/L) | MgSO4(1mM) | CaCl2(0.1mM) | 37 | 511145 | Escherichia coli MS56 | Illumina/ 50 | /var/data/MG1655.gbk | |
| A19 F999 I0 R1 | clonal | initially supplemented with 0.1% LB medium reduced in a stepwise manner to eventually achieve supplement-free growth. | M9 | glucose(2g/L) | NH4Cl(1g/L) | KH2PO4(3g/L),Na2HPO4(6.78g/L) | MgSO4(1mM) | CaCl2(0.1mM) | 37 | 511145 | Escherichia coli MS56 | Illumina/ 50 | /var/data/MG1655.gbk | |
| A20 F999 I0 R1 | clonal | initially supplemented with 0.1% LB medium reduced in a stepwise manner to eventually achieve supplement-free growth. | M9 | glucose(2g/L) | NH4Cl(1g/L) | KH2PO4(3g/L),Na2HPO4(6.78g/L) | MgSO4(1mM) | CaCl2(0.1mM) | 37 | 511145 | Escherichia coli MS56 | Illumina/ 50 | /var/data/MG1655.gbk | |
| A21 F999 I0 R1 | clonal | initially supplemented with 0.1% LB medium reduced in a stepwise manner to eventually achieve supplement-free growth. | M9 | glucose(2g/L) | NH4Cl(1g/L) | KH2PO4(3g/L),Na2HPO4(6.78g/L) | MgSO4(1mM) | CaCl2(0.1mM) | 37 | 511145 | Escherichia coli MS56 | Illumina/ 50 | /var/data/MG1655.gbk | |
| A22 F999 I0 R1 | clonal | initially supplemented with 0.1% LB medium reduced in a stepwise manner to eventually achieve supplement-free growth. | M9 | glucose(2g/L) | NH4Cl(1g/L) | KH2PO4(3g/L),Na2HPO4(6.78g/L) | MgSO4(1mM) | CaCl2(0.1mM) | 37 | 511145 | Escherichia coli MS56 | Illumina/ 50 | /var/data/MG1655.gbk | |
| A23 F999 I0 R1 | clonal | initially supplemented with 0.1% LB medium reduced in a stepwise manner to eventually achieve supplement-free growth. | M9 | glucose(2g/L) | NH4Cl(1g/L) | KH2PO4(3g/L),Na2HPO4(6.78g/L) | MgSO4(1mM) | CaCl2(0.1mM) | 37 | 511145 | Escherichia coli MS56 | Illumina/ 50 | /var/data/MG1655.gbk | |
| A24 F999 I0 R1 | clonal | initially supplemented with 0.1% LB medium reduced in a stepwise manner to eventually achieve supplement-free growth. | M9 | glucose(2g/L) | NH4Cl(1g/L) | KH2PO4(3g/L),Na2HPO4(6.78g/L) | MgSO4(1mM) | CaCl2(0.1mM) | 37 | 511145 | Escherichia coli MS56 | Illumina/ 50 | /var/data/MG1655.gbk | |
| A25 F999 I0 R1 | clonal | initially supplemented with 0.1% LB medium reduced in a stepwise manner to eventually achieve supplement-free growth. | M9 | glucose(2g/L) | NH4Cl(1g/L) | KH2PO4(3g/L),Na2HPO4(6.78g/L) | MgSO4(1mM) | CaCl2(0.1mM) | 37 | 511145 | Escherichia coli MS56 | Illumina/ 50 | /var/data/MG1655.gbk | |
| A26 F999 I0 R1 | clonal | initially supplemented with 0.1% LB medium reduced in a stepwise manner to eventually achieve supplement-free growth. | M9 | glucose(2g/L) | NH4Cl(1g/L) | KH2PO4(3g/L),Na2HPO4(6.78g/L) | MgSO4(1mM) | CaCl2(0.1mM) | 37 | 511145 | Escherichia coli MS56 | Illumina/ 50 | /var/data/MG1655.gbk | |
| A27 F999 I0 R1 | clonal | initially supplemented with 0.1% LB medium reduced in a stepwise manner to eventually achieve supplement-free growth. | M9 | glucose(2g/L) | NH4Cl(1g/L) | KH2PO4(3g/L),Na2HPO4(6.78g/L) | MgSO4(1mM) | CaCl2(0.1mM) | 37 | 511145 | Escherichia coli MS56 | Illumina/ 50 | /var/data/MG1655.gbk | |
| A28 F999 I0 R1 | clonal | initially supplemented with 0.1% LB medium reduced in a stepwise manner to eventually achieve supplement-free growth. | M9 | glucose(2g/L) | NH4Cl(1g/L) | KH2PO4(3g/L),Na2HPO4(6.78g/L) | MgSO4(1mM) | CaCl2(0.1mM) | 37 | 511145 | Escherichia coli MS56 | Illumina/ 50 | /var/data/MG1655.gbk | |
| A29 F999 I0 R1 | clonal | initially supplemented with 0.1% LB medium reduced in a stepwise manner to eventually achieve supplement-free growth. | M9 | glucose(2g/L) | NH4Cl(1g/L) | KH2PO4(3g/L),Na2HPO4(6.78g/L) | MgSO4(1mM) | CaCl2(0.1mM) | 37 | 511145 | Escherichia coli MS56 | Illumina/ 50 | /var/data/MG1655.gbk | |
| A30 F999 I0 R1 | clonal | initially supplemented with 0.1% LB medium reduced in a stepwise manner to eventually achieve supplement-free growth. | M9 | glucose(2g/L) | NH4Cl(1g/L) | KH2PO4(3g/L),Na2HPO4(6.78g/L) | MgSO4(1mM) | CaCl2(0.1mM) | 37 | 511145 | Escherichia coli MS56 | Illumina/ 50 | /var/data/MG1655.gbk | |
| A31 F999 I0 R1 | clonal | initially supplemented with 0.1% LB medium reduced in a stepwise manner to eventually achieve supplement-free growth. | M9 | glucose(2g/L) | NH4Cl(1g/L) | KH2PO4(3g/L),Na2HPO4(6.78g/L) | MgSO4(1mM) | CaCl2(0.1mM) | 37 | 511145 | Escherichia coli MS56 | Illumina/ 50 | /var/data/MG1655.gbk |