breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 12,086 C→T intergenic (‑300/‑77) yaaI ← / → dnaK DUF2541 domain‑containing protein YaaI/chaperone protein DnaK
MC JC 15,389 Δ5,175 bp insL‑1insA‑1 insL‑1, hokC, mokC, sokC, nhaA, nhaR, insB‑1, insA‑1
MC JC 92,854 (GCG)4→3 coding (1442‑1444/1767 nt) ftsI → peptidoglycan DD‑transpeptidase FtsI
RA 119,028 T→C V99A (GTC→GCC)  ampD → 1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase
MC JC 167,484 Δ5,964 bp [fhuA]fhuB [fhuA], fhuC, fhuD, fhuB
RA 214,885 C→T T18I (ACC→ATC)  arfB → peptidyl‑tRNA hydrolase, ribosome rescue factor
RA 220,099 A→G intergenic (‑104/+14) rcsF ← / ← metQ outer membrane lipoprotein RcsF/L‑methionine/D‑methionine ABC transporter membrane anchored binding protein
RA 257,794 T→C intergenic (+23/‑35) frsA → / → crl fermentation‑respiration switch protein/RNA polymerase holoenzyme assembly factor Crl
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
MC JC 263,514 Δ61,897 bp [ykfI]ykgH 71 genes
MC JC 332,366 Δ44,951 bp pdeLyaiL 39 genes
RA 380,022 (G)10→9 intergenic (‑141/+47) frmR ← / ← yaiO DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO
MC JC 389,898 Δ9,908 bp yaiT[yaiZ] yaiT, insF‑2, insE‑2, yaiT, iprA, ampH, sbmA, yaiW, yaiY, [yaiZ]
MC JC 508,873 Δ40,744 bp copA[ylbE] 37 genes
MC JC 565,055 Δ21,054 bp [intD]ompT 37 genes
MC JC 603,467 Δ5,885 bp [ybdG]insL‑2 [ybdG], nfsB, ybdF, ybdJ, ybdK, sokE, hokE, insL‑2
RA 612,647 C→T intergenic (‑153/‑90) fepA ← / → fes ferric enterobactin outer membrane transporter/enterochelin esterase
MC JC 675,552 Δ7,819 bp ybeQ[hscC] ybeQ, ybeR, djlB, ybeT, ybeU, djlC, [hscC]
MC JC 687,860 Δ1,185 bp IS5‑mediated insH‑3 insH‑3
MC JC 729,365 Δ9,598 bp rhsCybfD rhsC, ybfB, ybfO, ybfC, ybfQ, ybfL, ybfD
RA 765,191 Δ1 bp coding (685/723 nt) mngR ← DNA‑binding transcriptional repressor MngR
MC JC 765,208 Δ5,405 bp [mngR]mngB [mngR], mngA, mngB
MC JC 819,776 Δ62,977 bp ybhLdeoR 57 genes
RA 972,866 Δ1 bp coding (536/780 nt) elyC ← envelope biogenesis factor
RA 978,231 C→T L636L (CTG→TTG)  mukB → chromosome partitioning protein MukB
RA 988,499 G→A P163S (CCA→TCA)  asnS ← asparagine‑‑tRNA ligase
MC JC 997,868 Δ6,818 bp [elfA]ycbF [elfA], elfD, elfC, elfG, ycbU, ycbV, ycbF
MC JC 1,040,502 Δ73,763 bp [appX][mdtG] 75 genes
MC JC 1,129,397 Δ11,591 bp flgNflgL flgN, flgM, flgA, flgB, flgC, flgD, flgE, flgF, flgG, flgH, flgI, flgJ, flgK, flgL
RA 1,154,362 C→T A84V (GCC→GTC)  mltG → endolytic murein transglycosylase
MC JC 1,197,137 25956 bp→12 bp [ymfD][ymgI] 42 genes
RA 1,240,439 C→T V60V (GTC→GTT
D545N (GAC→AAC) 
ymgM →
cvrA ←
protein YmgM
putative K(+):H(+) antiporter
RA 1,270,811 C→T V347M (GTG→ATG)  chaA ← Na(+)/K(+):H(+) antiporter ChaA
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,382,555 C→T S203S (AGC→AGT ompG → outer membrane porin G
MC JC 1,388,889 Δ9,734 bp ycjG[uspE] ycjG, mpaA, ymjC, ymjD, ycjY, pgrR, mppA, ynaI, insH‑4, ynaJ, [uspE]
RA 1,400,309 Δ1 bp coding (1465/1527 nt) abgT ← p‑aminobenzoyl glutamate:H(+) symporter
RA 1,400,314 Δ1 bp coding (1460/1527 nt) abgT ← p‑aminobenzoyl glutamate:H(+) symporter
MC JC 1,400,325 Δ81,931 bp [abgT][azoR] 87 genes
MC JC 1,509,562 Δ46,201 bp [hicB]maeA 43 genes
RA 1,562,085 C→T A133T (GCA→ACA)  ddpA ← putative D,D‑dipeptide ABC transporter periplasmic binding protein
RA 1,626,996 C→T D129N (GAT→AAT)  dcp ← peptidyl‑dipeptidase Dcp
MC JC 1,627,518 Δ25,244 bp [ydfG][ynfP] 48 genes
RA 1,656,514 G→A G111S (GGC→AGC)  speG → spermidine N‑acetyltransferase
RA 1,662,872 C→T R773W (CGG→TGG)  ynfF → putative selenate reductase YnfF
RA 1,697,216 T→C intergenic (‑164/+57) uidR ← / ← hdhA DNA‑binding transcriptional repressor UidR/7‑alpha‑hydroxysteroid dehydrogenase
RA 1,741,062 T→C C384C (TGT→TGC ydhC → putative transporter YdhC
RA 1,742,755 G→C P163R (CCG→CGG)  ribC ← riboflavin synthase
RA 1,776,533 G→A G316D (GGC→GAC)  ydiF → putative acetate‑CoA transferase
RA 1,811,208 T→C V92A (GTT→GCT)  tcyP → cystine/sulfocysteine:cation symporter
RA 1,847,724 (C)6→7 coding (285/1044 nt) selD ← selenide, water dikinase
MC JC 1,872,030 Δ1,437 bp [dgcJ] [dgcJ]
RA 1,898,152 G→A A407A (GCG→GCA sdaA → L‑serine deaminase I
RA 1,907,399 C→T E13K (GAG→AAG)  cspC ← stress protein, member of the CspA family
RA 1,941,784 A→G V267A (GTC→GCC)  znuA ← Zn(2(+)) ABC transporter periplasmic binding protein
MC JC 1,962,566 Δ16,764 bp flhEinsA‑5 17 genes
MC JC 1,997,112 Δ26,567 bp [tcyN][fliR] 32 genes
RA 2,040,801 T→C L107L (TTA→CTA)  msrQ → periplasmic protein‑L‑methionine sulfoxide reductase heme binding subunit
MC JC 2,101,397 Δ36,321 bp [wbbL]wza 34 genes
MC JC 2,165,151 Δ12,060 bp yegPgatY 17 genes
MC JC 2,204,350 Δ32,368 bp yehLpreA 30 genes
MC JC 2,286,401 Δ3,780 bp [yejO][yejO] [yejO], insH‑8, [yejO]
MC JC 2,320,144 Δ16,489 bp [atoS][yfaA] [atoS], atoC, atoD, atoA, atoE, atoB, yfaP, yfaQ, yfaS, yfaT, [yfaA]
RA 2,406,141 A→G R167R (CGT→CGC lrhA ← DNA‑binding transcriptional dual regulator LrhA
MC JC 2,466,545 Δ9,634 bp [intS][ypdJ] 15 genes
MC JC 2,509,631 Δ8,319 bp [yfeO]pdeA [yfeO], ypeC, mntH, nupC, insL‑3, pdeA
MC JC 2,558,699 Δ6,790 bp intZ[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
MC JC 2,601,201 Δ13,595 bp [hyfA]focB [hyfA], hyfB, hyfC, hyfD, hyfE, hyfF, hyfG, hyfH, hyfI, hyfJ, hyfR, focB
RA 2,662,004 T→C E43G (GAA→GGA)  iscR ← DNA‑binding transcriptional dual regulator IscR
RA 2,756,138 Δ1 bp intergenic (+183/‑21) ssrA → / → intA tmRNA/CP4‑57 prophage; integrase
MC JC 2,756,160 Δ35,091 bp [intA][lhgO] 33 genes
RA 2,793,867 C→A A378D (GCT→GAT)  gabT → 4‑aminobutyrate aminotransferase GabT
MC JC 2,798,370 (TTC)4→3 coding (124‑126/405 nt) stpA ← DNA‑binding transcriptional repressor StpA with RNA chaperone activity
RA 2,813,364 T→C V250A (GTC→GCC)  emrB → multidrug efflux pump membrane subunit EmrB
RA 2,843,412 C→T R12H (CGT→CAT)  hycH ← formate hydrogenlyase assembly protein
MC JC 2,846,301 Δ20,968 bp [hycE][rpoS] 21 genes
MC JC 2,994,992 Δ3,877 bp [ygeL]ygeQ [ygeL], ygeN, ygeO, insD‑4, insC‑4, ygeP, ygeQ
RA 3,090,282 C→T intergenic (+30/‑65) yggI → / → endA conserved protein YggI/DNA‑specific endonuclease I
RA 3,090,341 Δ1 bp intergenic (+89/‑6) yggI → / → endA conserved protein YggI/DNA‑specific endonuclease I
MC JC 3,090,347 Δ708 bp coding (1‑708/708 nt) endA → DNA‑specific endonuclease I
RA 3,091,305 A→G S58G (AGC→GGC)  rsmE → 16S rRNA m(3)U1498 methyltransferase
RA 3,093,157 T→C L74P (CTC→CCC)  yqgE → DUF179 domain‑containing protein YqgE
MC JC 3,110,680 25702 bp→GA [yghD]pitB 20 genes
MC JC 3,184,782 Δ6,916 bp ubiKyqiI ubiK, ygiL, yqiG, insC‑5, insD‑5, yqiG, yqiH, yqiI
RA 3,213,803 T→C S253P (TCT→CCT)  rpoD → RNA polymerase, sigma 70 (sigma D) factor
RA 3,240,984 (G)7→8 coding (1041/1245 nt) sstT → serine/threonine:Na(+) symporter
RA 3,354,900 T→A L59* (TTA→TAA)  gltB → glutamate synthase subunit GltB
MC JC 3,362,162 Δ5,481 bp [yhcA][yhcF] [yhcA], yhcD, yhcE, insH‑10, yhcE, [yhcF]
MC JC 3,453,928 Δ15,926 bp gspA[chiA] 16 genes
RA 3,477,150 C→T K90K (AAG→AAA fkpA ← peptidyl‑prolyl cis‑trans isomerase FkpA
RA 3,513,003 A→G D212D (GAT→GAC trpS ← tryptophan‑‑tRNA ligase
RA 3,527,760 A→G N431S (AAC→AGC)  igaA → inner membrane protein ‑ inhibits the Rcs signaling pathway
MC JC 3,581,139 3915 bp→6 bp [yhhY]yrhB [yhhY], yhhZ, yrhA, insA‑6, insB‑6, yrhA, yrhD, yrhB
MC JC 3,618,992 Δ6,688 bp rhsB[yhhJ] rhsB, yhhH, yhhI, [yhhJ]
MC JC 3,651,293 Δ2,422 bp [yhiS]yhiS [yhiS], insH‑11, yhiS
RA 3,720,597 Δ1 bp coding (4/153 nt) hokA ← small toxic polypeptide
MC JC 3,720,634 Δ1,442 bp IS150‑mediated insJinsK insJ, insK
MC JC 3,761,993 Δ8,250 bp rhsAyibV rhsA, yibA, yibJ, yibG, yibS, yibV
RA 3,801,078 (T)7→6 coding (922/1017 nt) waaJ ← UDP‑glucose:(glucosyl)LPS alpha‑1,2‑glucosyltransferase
RA 3,816,579 G→A intergenic (‑30/‑97) rph ← / → yicC truncated RNase PH/UPF0701 family protein YicC
RA 3,829,233 C→A L97M (CTG→ATG)  xanP → xanthine:H(+) symporter XanP
RA 3,850,891 G→A Q68* (CAA→TAA)  ilvN ← acetohydroxy acid synthase I subunit IlvN
MC JC 3,888,432 Δ18,167 bp tnaCbglG 17 genes
RA 3,926,696 C→T G103D (GGC→GAC)  asnC ← DNA‑binding transcriptional dual regulator AsnC
RA 3,948,805 (G)6→7 coding (1165/1521 nt) yifB ← putative magnesium chelatase YifB
RA 3,979,769 C→T A390V (GCG→GTG)  wzyE → enterobacterial common antigen polymerase
RA 3,980,957 (G)6→7 coding (71/1386 nt) yifK → putative transporter YifK
RA 4,034,771 C→T A55V (GCT→GTT)  hemG → protoporphyrinogen oxidase
RA 4,093,223 A→G V72V (GTT→GTC rhaM ← L‑rhamnose mutarotase
MC JC 4,299,628 Δ25,538 bp [mdtP][phnC] 27 genes
RA 4,354,915 G→A intergenic (‑198/+39) lysU ← / ← dtpC lysine‑‑tRNA ligase/Ap4A synthetase/Ap3A synthetase/dipeptide/tripeptide:H(+) symporter DtpC
RA 4,438,124 T→C W140R (TGG→CGG)  ytfI → protein YtfI
RA 4,485,906 C→A A16S (GCC→TCC)  pepA ← aminopeptidase A/I
MC JC 4,496,676 Δ53,036 bp intBfimH 54 genes
MC JC 4,555,491 Δ41,523 bp [yjiC]lgoD 37 genes
MC JC 4,626,183 (GCG)3→2 coding (272‑274/1383 nt) radA → DNA recombination protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 380112 388670 8559 24 [0] [6] 7 [yaiO]–tauD [yaiO],yaiX,insC‑1,insD‑1,yaiX,yaiP,ykiC,yaiS,tauA,tauB,tauC,tauD
* * ÷ NC_000913 2066216–2066301 2080591 14291–14376 9 [6] [0] 23 insH‑6–[yeeA] insH‑6,yoeA,insD‑3,insC‑3,yoeA,yeeP,isrC,flu,yeeR,yeeS,yeeT,cbeA,cbtA,yeeW,yoeF,yeeX,[yeeA]
* * ÷ NC_000913 3423698–3424234 3428522–3424653 420–4825 8 [6] [6] 8 [rrfD]–[rrsD] [rrfD],rrlD,alaU,ileU,[rrsD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 226599NA (NA)24 (1.200)
+GACGAATACCATT
15/72 0.1 NA noncoding (841/2904 nt) rrlH 23S ribosomal RNA
?NC_000913 226613 = NA (NA)noncoding (855/2904 nt) rrlH 23S ribosomal RNA
* ? NC_000913 = 3801110 (0.000)20 (0.950)
+CGAGATGGACG
17/76 0.1 63.4% coding (732/774 nt) yaiO outer membrane protein YaiO
?NC_000913 624576 = 30 (1.090)intergenic (‑66/‑309) fepB/entC ferric enterobactin ABC transporter periplasmic binding protein/isochorismate synthase EntC
* ? NC_000913 = 2066304NA (NA)23 (0.840) 21/98 0.1 100% noncoding (1050/1195 nt) IS5 repeat region
?NC_000913 2080592 = 0 (0.000)intergenic (‑1/+197) yeeA/sbmC putative transporter YeeA/DNA gyrase inhibitor