breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 12,086 C→T intergenic (‑300/‑77) yaaI ← / → dnaK DUF2541 domain‑containing protein YaaI/chaperone protein DnaK
RA 119,028 T→C V99A (GTC→GCC)  ampD → 1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase
RA 257,794 T→C intergenic (+23/‑35) frsA → / → crl fermentation‑respiration switch protein/RNA polymerase holoenzyme assembly factor Crl
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
MC JC 263,514 Δ61,897 bp [ykfI]ykgH 71 genes
RA 367,573 G→A intergenic (‑63/+14) lacI ← / ← mhpR DNA‑binding transcriptional repressor LacI/DNA‑binding transcriptional activator MhpR
RA 380,022 (G)10→9 intergenic (‑141/+47) frmR ← / ← yaiO DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO
RA 765,191 Δ1 bp coding (685/723 nt) mngR ← DNA‑binding transcriptional repressor MngR
RA 988,499 G→A P163S (CCA→TCA)  asnS ← asparagine‑‑tRNA ligase
RA 1,197,502 C→T G11R (GGA→AGA)  ymfD ← e14 prophage; putative SAM‑dependent methyltransferase
RA 1,270,811 C→T V347M (GTG→ATG)  chaA ← Na(+)/K(+):H(+) antiporter ChaA
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,382,555 C→T S203S (AGC→AGT ompG → outer membrane porin G
RA 1,400,309 Δ1 bp coding (1465/1527 nt) abgT ← p‑aminobenzoyl glutamate:H(+) symporter
RA 1,400,314 Δ1 bp coding (1460/1527 nt) abgT ← p‑aminobenzoyl glutamate:H(+) symporter
RA 1,544,022 1 bp→AA coding (39/1389 nt) narU ← nitrate/nitrite transporter NarU
RA 1,742,755 G→C P163R (CCG→CGG)  ribC ← riboflavin synthase
MC JC 1,962,566 Δ16,764 bp flhEinsA‑5 17 genes
MC JC 2,101,397 Δ36,321 bp [wbbL]wza 34 genes
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,406,141 A→G R167R (CGT→CGC lrhA ← DNA‑binding transcriptional dual regulator LrhA
RA 2,756,138 Δ1 bp intergenic (+183/‑21) ssrA → / → intA tmRNA/CP4‑57 prophage; integrase
RA 2,793,867 C→A A378D (GCT→GAT)  gabT → 4‑aminobutyrate aminotransferase GabT
JC 2,798,370 (TTC)4→3 coding (124‑126/405 nt) stpA ← DNA‑binding transcriptional repressor StpA with RNA chaperone activity
RA 2,843,412 C→T R12H (CGT→CAT)  hycH ← formate hydrogenlyase assembly protein
MC JC 2,994,992 Δ3,877 bp [ygeL]ygeQ [ygeL], ygeN, ygeO, insD‑4, insC‑4, ygeP, ygeQ
RA 3,090,282 C→T intergenic (+30/‑65) yggI → / → endA conserved protein YggI/DNA‑specific endonuclease I
RA 3,090,341 Δ1 bp intergenic (+89/‑6) yggI → / → endA conserved protein YggI/DNA‑specific endonuclease I
RA 3,091,305 A→G S58G (AGC→GGC)  rsmE → 16S rRNA m(3)U1498 methyltransferase
RA 3,213,803 T→C S253P (TCT→CCT)  rpoD → RNA polymerase, sigma 70 (sigma D) factor
RA 3,477,150 C→T K90K (AAG→AAA fkpA ← peptidyl‑prolyl cis‑trans isomerase FkpA
RA 3,513,003 A→G D212D (GAT→GAC trpS ← tryptophan‑‑tRNA ligase
RA 3,720,597 Δ1 bp coding (4/153 nt) hokA ← small toxic polypeptide
RA 3,801,078 (T)7→6 coding (922/1017 nt) waaJ ← UDP‑glucose:(glucosyl)LPS alpha‑1,2‑glucosyltransferase
RA 3,829,233 C→A L97M (CTG→ATG)  xanP → xanthine:H(+) symporter XanP
RA 3,850,891 G→A Q68* (CAA→TAA)  ilvN ← acetohydroxy acid synthase I subunit IlvN
RA 3,948,805 (G)6→7 coding (1165/1521 nt) yifB ← putative magnesium chelatase YifB
RA 4,093,223 A→G V72V (GTT→GTC rhaM ← L‑rhamnose mutarotase
RA 4,354,915 G→A intergenic (‑198/+39) lysU ← / ← dtpC lysine‑‑tRNA ligase/Ap4A synthetase/Ap3A synthetase/dipeptide/tripeptide:H(+) symporter DtpC
RA 4,438,124 T→C W140R (TGG→CGG)  ytfI → protein YtfI
RA 4,485,906 C→A A16S (GCC→TCC)  pepA ← aminopeptidase A/I
MC JC 4,496,676 Δ53,036 bp intBfimH 54 genes

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 2066205–2066464 2080591 14128–14387 69 [68] [8] 129 insH‑6–[yeeA] insH‑6,yoeA,insD‑3,insC‑3,yoeA,yeeP,isrC,flu,yeeR,yeeS,yeeT,cbeA,cbtA,yeeW,yoeF,yeeX,[yeeA]
* * ÷ NC_000913 3423691–3424234 3428528–3424238 5–4838 71 [68] [68] 78 [rrfD]–[rrsD] [rrfD],rrlD,alaU,ileU,[rrsD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 153888 (0.070)92 (0.860) 48/96 0.5 86.5% noncoding (2/1345 nt) IS186 repeat region
?NC_000913 20564 = 21 (0.200)intergenic (‑56/+251) insA‑1/rpsT IS1 protein InsA/30S ribosomal subunit protein S20
* ? NC_000913 = 16748315 (0.140)164 (1.500) 84/98 0.0 91.4% intergenic (+219/‑1) mrcB/fhuA peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcB/ferrichrome outer membrane transporter/phage receptor
?NC_000913 173448 = 16 (0.150)intergenic (+4/+154) fhuB/hemL iron(III) hydroxamate ABC transporter membrane subunit/glutamate‑1‑semialdehyde aminotransferase
* ? NC_000913 = 33236513 (0.120)168 (1.530) 82/98 0.0 92.8% intergenic (+489/‑6) yahV/pdeL protein YahV/DNA‑binding transcriptional activator/c‑di‑GMP phosphodiesterase PdeL
?NC_000913 377317 = NA (NA)intergenic (+6/+218) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 366308 =39 (0.360)193 (1.880)
+ACC
73/92 0.0 68.9% intergenic (‑3/+120) lacZ/lacI beta‑galactosidase/DNA‑binding transcriptional repressor LacI
?NC_000913 = 1795552 147 (1.340)coding (1/300 nt) ihfA integration host factor subunit alpha
* ? NC_000913 = 36636456 (0.510)146 (1.390) 67/94 0.0 59.3% intergenic (‑59/+64) lacZ/lacI beta‑galactosidase/DNA‑binding transcriptional repressor LacI
?NC_000913 1323131 = 147 (1.400)intergenic (‑49/‑89) trpL/yciV trp operon leader peptide/RNA/ssDNA 5'‑>3' exonuclease
* ? NC_000913 = 3801115 (0.050)86 (0.900)
+CGAGAT
47/86 0.2 62.3% coding (732/774 nt) yaiO outer membrane protein YaiO
?NC_000913 4070444 = 114 (1.040)intergenic (‑43/+71) yihR/yihS putative aldose 1‑epimerase YihR/sulfoquinovose isomerase
* ? NC_000913 = 3898977 (0.060)151 (1.380) 76/98 0.0 94.7% intergenic (‑170/‑354) hemB/yaiT porphobilinogen synthase/putative autotransporter YaiT
?NC_000913 399806 = 10 (0.090)intergenic (+1/+23) yaiZ/ddlA DUF2754 domain‑containing protein YaiZ/D‑alanine‑‑D‑alanine ligase A
* ? NC_000913 = 50887210 (0.090)151 (1.380) 76/98 0.0 93.5% intergenic (+313/+3) ybaQ/copA putative DNA‑binding transcriptional regulator YbaQ/Cu(+) exporting P‑type ATPase
?NC_000913 549617 = 11 (0.100)intergenic (+1/‑10) ylbE/ylbF DUF1116 domain‑containing protein YlbE/DUF2877 domain‑containing protein YlbF
* ? NC_000913 = 56505411 (0.100)108 (1.100) 67/88 0.0 91.6% coding (925/1164 nt) intD DLP12 prophage; putative integrase
?NC_000913 586109 = 10 (0.100)intergenic (‑476/+38) ompT/envY outer membrane protease VII (outer membrane protein 3b)/DNA‑binding transcriptional activator EnvY
* ? NC_000913 = 60346612 (0.110)119 (1.130) 65/94 0.0 92.4% coding (1198/1248 nt) ybdG miniconductance mechanosensitive channel YbdG
?NC_000913 609352 = 8 (0.080)intergenic (+175/+107) insL‑2/entD IS186/IS421 transposase/phosphopantetheinyl transferase EntD
* ? NC_000913 = 67555113 (0.120)128 (1.170) 63/98 0.0 90.8% intergenic (+51/+19) ybeL/ybeQ DUF1451 domain‑containing protein YbeL/Sel1 repeat‑containing protein YbeQ
?NC_000913 683371 = 13 (0.120)coding (23/1671 nt) hscC chaperone protein HscC
* ? NC_000913 = 6878599 (0.080)143 (1.330) 67/96 0.0 92.9% noncoding (1187/1195 nt) IS5 repeat region
?NC_000913 689045 = 13 (0.120)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 = 7293645 (0.050)121 (1.150) 66/94 0.0 95.3% intergenic (+24/‑219) ybfA/rhsC DUF2517 domain‑containing protein YbfA/rhs element protein RhsC
?NC_000913 738963 = 7 (0.070)intergenic (+110/‑38) ybfD/ybgA H repeat‑associated putative transposase YbfD/DUF1722 domain‑containing protein YbgA
* ? NC_000913 = 76520714 (0.130)92 (0.860) 51/96 0.3 87.7% coding (669/723 nt) mngR DNA‑binding transcriptional repressor MngR
?NC_000913 770613 = 12 (0.110)intergenic (+2/‑845) mngB/cydA alpha‑mannosidase/cytochrome bd‑I ubiquinol oxidase subunit I
* ? NC_000913 = 819775NA (NA)127 (1.160) 67/98 0.0 89.4% noncoding (17/27 nt) REPt74 REPt74
?NC_000913 882753 = 15 (0.140)intergenic (‑19/+39) deoR/ybjG DNA‑binding transcriptional repressor DeoR/undecaprenyl pyrophosphate phosphatase
* ? NC_000913 = 9978676 (0.050)134 (1.220) 66/98 0.0 94.0% intergenic (‑355/‑1) ssuE/elfA NADPH‑dependent FMN reductase/putative laminin‑binding fimbrial subunit
?NC_000913 1004686 = 11 (0.100)intergenic (+29/‑82) ycbF/pyrD putative fimbrial chaperone YcbF/dihydroorotate dehydrogenase, type 2
* ? NC_000913 = 10405019 (0.080)113 (1.050) 59/96 0.1 93.5% coding (57/93 nt) appX small protein AppX
?NC_000913 1114265 = 7 (0.070)coding (1226/1227 nt) mdtG efflux pump MdtG
* ? NC_000913 = 11293968 (0.070)98 (0.890) 66/98 0.0 90.7% intergenic (+22/+18) murJ/flgN putative lipid II flippase MurJ/flagellar biosynthesis protein FlgN
?NC_000913 1140988 = 12 (0.110)intergenic (+2/+194) flgL/rne flagellar hook‑filament junction protein 2/ribonuclease E
* ? NC_000913 = 11971369 (0.080)97 (1.180)
+CAGATAATTCAA
54/74 0.0 92.5% coding (397/666 nt) ymfD e14 prophage; putative SAM‑dependent methyltransferase
?NC_000913 1223093 = 12 (0.110)coding (71/174 nt) ymgI uncharacterized protein YmgI
* ? NC_000913 = 138888813 (0.120)112 (1.040) 67/96 0.0 91.5% intergenic (‑77/‑42) tpx/ycjG lipid hydroperoxide peroxidase/L‑Ala‑D/L‑Glu epimerase
?NC_000913 1398623 = 8 (0.070)intergenic (‑1/+151) uspE/fnr universal stress protein with a role cellular motility/DNA‑binding transcriptional dual regulator FNR
* ? NC_000913 = 140032410 (0.090)111 (1.010) 57/98 0.1 90.6% coding (1450/1527 nt) abgT p‑aminobenzoyl glutamate:H(+) symporter
?NC_000913 1482256 = 13 (0.120)coding (605/606 nt) azoR FMN dependent NADH:quinone oxidoreductase
* ? NC_000913 = 15095619 (0.080)134 (1.220) 70/98 0.0 92.7% coding (54/417 nt) hicB antitoxin of the HicA‑HicB toxin‑antitoxin system
?NC_000913 1555763 = 12 (0.110)intergenic (‑94/+63) maeA/sra malate dehydrogenase, NAD‑requiring/30S ribosomal subunit protein S22
* ? NC_000913 = 16275178 (0.070)135 (1.260) 68/96 0.0 93.8% coding (1/747 nt) ydfG 3‑hydroxy acid dehydrogenase
?NC_000913 1652762 = 10 (0.090)coding (77/111 nt) ynfP protein YnfP
* ? NC_000913 = 18720297 (0.060)130 (1.210) 66/96 0.0 93.6% intergenic (+169/‑12) cdgI/dgcJ putative c‑di‑GMP binding protein CdgI/putative diguanylate cyclase DgcJ
?NC_000913 1873467 = 11 (0.100)coding (1427/1491 nt) dgcJ putative diguanylate cyclase DgcJ
* ? NC_000913 = 19971119 (0.080)59 (0.540) 42/98 1.1 84.9% coding (704/753 nt) tcyN cystine ABC transporter ATP binding subunit
?NC_000913 2023679 = 12 (0.110)intergenic (+1/‑289) fliR/rcsA flagellar biosynthesis protein FliR/DNA‑binding transcriptional activator RcsA
* ? NC_000913 = 2066304NA (NA)119 (1.090) 67/98 0.0 93.7% noncoding (1050/1195 nt) IS5 repeat region
?NC_000913 2080592 = 8 (0.070)intergenic (‑1/+197) yeeA/sbmC putative transporter YeeA/DNA gyrase inhibitor
* ? NC_000913 = 216515013 (0.120)147 (1.340) 65/98 0.0 91.9% intergenic (+152/‑39) baeR/yegP DNA‑binding transcriptional activator BaeR/DUF1508 domain‑containing protein YegP
?NC_000913 2177211 = 13 (0.120)intergenic (‑7/+301) gatY/fbaB tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
* ? NC_000913 = 220434910 (0.090)107 (0.980) 61/98 0.0 90.3% intergenic (+60/‑247) yehK/yehL uncharacterized protein YehK/putative AAA(+) MoxR family ATPase YehL
?NC_000913 2236718 = 13 (0.120)intergenic (+218/+25) preA/mglC NAD‑dependent dihydropyrimidine dehydrogenase subunit PreA/D‑galactose/methyl‑galactoside ABC transporter membrane subunit
* ? NC_000913 = 22864009 (0.080)139 (1.270) 75/98 0.0 93.0% pseudogene (2515/2525 nt) yejO adhesin‑like autotransporter YejO
?NC_000913 2290181 = 12 (0.110)intergenic (‑1/‑319) yejO/narP adhesin‑like autotransporter YejO/DNA‑binding transcriptional dual regulator NarP
* ? NC_000913 = 23201438 (0.070)132 (1.230) 74/96 0.0 93.3% coding (101/1827 nt) atoS sensory histidine kinase AtoS
?NC_000913 2336633 = 11 (0.100)coding (12/1689 nt) yfaA DUF2138 domain‑containing protein YfaA
* ? NC_000913 = 246654414 (0.130)157 (1.430) 75/98 0.0 91.5% intergenic (+161/‑1) argW/intS tRNA‑Arg/CPS‑53 (KpLE1) prophage; prophage CPS‑53 integrase
?NC_000913 2476179 = 15 (0.140)coding (86/141 nt) ypdJ CPS‑53 (KpLE1) prophage; putative uncharacterized protein YpdJ
* ? NC_000913 = 25096309 (0.080)117 (1.070) 62/98 0.0 92.5% coding (1/1257 nt) yfeO putative transport protein YfeO
?NC_000913 2517950 = 10 (0.090)intergenic (‑118/+91) pdeA/alaX putative c‑di‑GMP phosphodiesterase PdeA/tRNA‑Ala
* ? NC_000913 = 255869811 (0.100)100 (1.100) 62/82 0.0 91.2% intergenic (‑19/‑160) eutB/intZ ethanolamine ammonia‑lyase subunit alpha/CPZ‑55 prophage; putative phage integrase IntZ
?NC_000913 2565489 = 10 (0.110)coding (1396/1404 nt) eutA reactivating factor for ethanolamine ammonia‑lyase EutBC
* ? NC_000913 = 260120013 (0.120)146 (1.330) 67/98 0.0 94.2% intergenic (+252/‑1) bcp/hyfA thiol peroxidase/hydrogenase 4 component A
?NC_000913 2614796 = 5 (0.050)intergenic (+14/+24) focB/yfgO putative formate transporter/putative transporter YfgO
* ? NC_000913 = 275615913 (0.120)133 (1.240) 64/96 0.0 91.2% coding (1/1242 nt) intA CP4‑57 prophage; integrase
?NC_000913 2791251 = 13 (0.120)intergenic (+1/‑22) lhgO/gabD L‑2‑hydroxyglutarate oxidase/NADP(+)‑dependent succinate‑semialdehyde dehydrogenase
* ? NC_000913 = 284630028 (0.260)101 (0.980) 59/92 0.0 81.0% coding (172/1710 nt) hycE formate hydrogenlyase subunit HycE
?NC_000913 2867269 = 21 (0.200)coding (283/993 nt) rpoS RNA polymerase, sigma S (sigma 38) factor
* ? NC_000913 = 309034612 (0.110)138 (1.260) 65/98 0.0 92.6% intergenic (+94/‑1) yggI/endA conserved protein YggI/DNA‑specific endonuclease I
?NC_000913 3091055 = 10 (0.090)intergenic (+1/‑79) endA/rsmE DNA‑specific endonuclease I/16S rRNA m(3)U1498 methyltransferase
* ? NC_000913 = 311067913 (0.120)81 (0.770)
+GA
49/94 0.4 88.9% coding (448/537 nt) yghD putative type II secretion system M‑type protein
?NC_000913 3136382 = 8 (0.070)intergenic (‑11/+281) pitB/gss metal phosphate:H(+) symporter PitB/fused glutathionylspermidine amidase/glutathionylspermidine synthetase
* ? NC_000913 = 318478113 (0.120)164 (1.530) 80/96 0.0 92.2% intergenic (‑315/‑59) ribB/ubiK 3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase/ubiquinone biosynthesis accessory factor UbiK
?NC_000913 3191698 = 15 (0.140)intergenic (+2/+41) yqiI/glgS putative fimbrial protein YqiI/surface composition regulator
* ? NC_000913 = 33621618 (0.070)135 (1.230) 69/98 0.0 91.5% coding (50/675 nt) yhcA putative fimbrial chaperone YhcA
?NC_000913 3367643 = 17 (0.160)intergenic (+1/‑184) yhcF/yhcG DUF1120 domain‑containing protein YhcF/DUF1016 domain‑containing protein YhcG
* ? NC_000913 = 345392719 (0.170)76 (0.740) 46/92 0.5 86.5% coding (1/420 nt) gspB putative general secretion pathway protein B
?NC_000913 3469854 = 6 (0.060)intergenic (‑1/+291) chiA/tufA endochitinase/translation elongation factor Tu 1
* ? NC_000913 = 358113819 (0.170)117 (1.220)
+CAAATG
60/86 0.0 88.1% coding (1/489 nt) yhhY N‑acetyltransferase YhhY
?NC_000913 3585054 = 17 (0.160)intergenic (+11/+27) yrhB/ggt putative heat shock chaperone/glutathione hydrolase proenzyme
* ? NC_000913 = 36189915 (0.050)115 (1.070) 65/96 0.0 95.9% intergenic (+2/‑201) nikR/rhsB DNA‑binding transcriptional repressor NikR/rhs element protein RhsB
?NC_000913 3625680 = NA (NA)coding (1124/1125 nt) yhhJ ABC transporter family protein YhhJ
* ? NC_000913 = 365129211 (0.100)109 (1.040) 64/94 0.0 91.8% pseudogene (2/746 nt) yhiS putative uncharacterized protein YhiS
?NC_000913 3653715 = 9 (0.090)intergenic (+2/‑246) yhiS/slp putative uncharacterized protein YhiS/starvation lipoprotein
* ? NC_000913 = 37206339 (0.080)104 (0.950) 54/98 0.2 90.4% noncoding (1/1443 nt) IS150 repeat region
?NC_000913 3722076 = 13 (0.120)intergenic (+27/+252) insK/glyS IS150 conserved protein InsB/glycine‑‑tRNA ligase subunit beta
* ? NC_000913 = 376199210 (0.090)132 (1.230) 65/96 0.0 91.7% intergenic (‑37/‑191) yibF/rhsA glutathione transferase‑like protein YibF/rhs element protein RhsA
?NC_000913 3770243 = 14 (0.130)coding (1137/1137 nt) yibH inner membrane protein YibH
* ? NC_000913 = 388843120 (0.180)84 (0.780) 55/96 0.1 85.0% intergenic (+239/‑4) mnmE/tnaC 5‑carboxymethylaminomethyluridine‑tRNA synthase GTPase subunit/tnaAB operon leader peptide
?NC_000913 3906599 = 10 (0.090)intergenic (‑32/+254) bglG/phoU transcriptional antiterminator BglG/negative regulator of the pho regulon
* ? NC_000913 = 42996279 (0.080)111 (1.010) 62/98 0.0 91.4% coding (1404/1467 nt) mdtP putative multidrug efflux pump outer membrane channel
?NC_000913 4325166 = 12 (0.110)intergenic (‑1/+132) phnC/yjdN phosphonate ABC transporter ATP binding subunit/conserved protein YjdN
* ? NC_000913 = 455549011 (0.100)136 (1.270) 70/96 0.0 91.3% coding (831/831 nt) yjiC uncharacterized protein YjiC
?NC_000913 4597014 = 15 (0.140)intergenic (+2/+136) lgoD/opgB L‑galactonate oxidoreductase/phosphoglycerol transferase I