Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A29 F999 I0 R1
|
9 |
7.7 |
636200 |
44.2% |
281200 |
128.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,604,716 |
C→A |
A186E (GCA→GAA) |
bglJ → |
DNA‑binding transcriptional regulator BglJ |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,604,716 | 0 | C | A | 100.0%
| 28.8
/ NA
| 14 | A186E (GCA→GAA) | bglJ | DNA‑binding transcriptional regulator BglJ |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (6/8); total (6/8) |
TGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGC‑GCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAG‑TAATGTGTTGCATCCCTA > NC_000913/4604592‑4604833
|
ggggaaaTTACCCAAAG‑CGGAATTTTAGCCCGGCGGAGAGGGAAATTTTTC‑GCTTTTTGGCCCTTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATa < 1:449377/123‑1 (MQ=255)
tAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGC‑GCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTaa > 1:623308/1‑129 (MQ=255)
aaCCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGC‑GCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCCAAACGATCCGTGAACATAAATTTAAt > 1:312480/1‑129 (MQ=255)
aCGGAGAGAGAAATATTGC‑GCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGAGGTCGAAACTGGGCgcaggt > 1:203686/1‑124 (MQ=255)
aaaaTAATGCGGCGTTAGGTCGTGGGGCAACTGAA‑GACACAAATTGCCTGTCAGCTTAAACGCAATATCAAAACGTTCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCa < 1:535825/128‑1 (MQ=255)
aaaTGACACAAATTGCCGGGCAGCTTAAACGCAATGTCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTtat < 1:287279/128‑1 (MQ=255)
aTGACACAAATTGCCCGGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTtatgt < 1:601949/129‑1 (MQ=255)
aaaTTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTTAACATAAATTTAATGTGATGTCGCAACTGGGCGTCAGTTCTGACGCAGGGTTGTTCGAGGCCGCAGCAATTCTGTTATTTATGccc > 1:459428/1‑126 (MQ=255)
aatttgcGAGCAACTTAAACGCAAAATCAAAACGGTCCCTGAAAATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCa < 1:267156/123‑1 (MQ=255)
cgaaaTACCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAG‑TAAtgtg < 1:629961/126‑1 (MQ=255)
aaTATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCGGTTATGAC‑CCGGGTTTGTGGGGGCCGCAGACATTCCTTTCAGATTGGGGCATTGGGCAACAAGATAAGGTGttc > 1:152146/1‑128 (MQ=255)
aaTATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAG‑TAATgtgt < 1:322765/127‑1 (MQ=255)
atacAAAACGGTCCGTGAACATAAATTTAATGGGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAG‑TAATGTGTTGc < 1:240807/125‑1 (MQ=255)
aaCGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAACCAAT‑TAAGTGGTTTCATCCCTa > 1:600356/1‑129 (MQ=255)
|
TGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGC‑GCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAG‑TAATGTGTTGCATCCCTA > NC_000913/4604592‑4604833
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
