Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A20 F999 I0 R1
|
98 |
154.1 |
11406411 |
97.3% |
11098437 |
49.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,926,760 |
A→G |
Y82H (TAC→CAC) |
asnC ← |
DNA‑binding transcriptional dual regulator AsnC |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,926,760 | 0 | A | G | 75.3%
| 301.5
/ 113.5
| 154 | Y82H (TAC→CAC) | asnC | DNA‑binding transcriptional dual regulator AsnC |
| Reads supporting (aligned to +/- strand): ref base A (17/21); new base G (49/67); total (66/88) |
| Fisher's exact test for biased strand distribution p-value = 8.51e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.48e-01 |
Only 100 of 159 total aligned reads displayed.
GCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCAT > NC_000913/3926711‑3926807
|
gCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTa < 1:2237507/50‑1 (MQ=255)
ttCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGggtggt > 1:253927/1‑50 (MQ=255)
cAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCt > 2:2979625/1‑50 (MQ=255)
cAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAACGCGGACGGGTAGt < 1:2642864/48‑1 (MQ=255)
aGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCtt > 2:3645788/1‑50 (MQ=255)
aGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCtt > 2:4915971/1‑50 (MQ=255)
aGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCtt < 1:2993692/50‑1 (MQ=255)
aGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCtt < 1:4701323/50‑1 (MQ=255)
aGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCtt < 1:5662481/50‑1 (MQ=255)
aGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCtt > 2:5552619/1‑50 (MQ=255)
aGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCtt < 2:1236181/50‑1 (MQ=255)
aGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCt < 2:3428755/49‑1 (MQ=255)
aGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCt < 2:503161/49‑1 (MQ=255)
gTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCttt < 1:1602197/50‑1 (MQ=255)
gTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCttt < 1:849154/50‑1 (MQ=255)
gTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCttt < 2:4617932/50‑1 (MQ=255)
tGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTg < 1:248529/50‑1 (MQ=255)
tGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTg < 2:4571323/50‑1 (MQ=255)
tGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTg > 2:1894398/1‑50 (MQ=255)
tGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTg < 1:5320429/50‑1 (MQ=255)
gACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTgg > 1:4999770/1‑50 (MQ=255)
aCTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGgc < 1:1954935/50‑1 (MQ=255)
cTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGgcg < 1:4086062/50‑1 (MQ=255)
cTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGgcg > 1:3765399/1‑50 (MQ=255)
cTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGgcg > 1:2652558/1‑50 (MQ=255)
ttCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGgcgc > 2:390051/1‑50 (MQ=255)
ttCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGgcgc > 2:1116916/1‑50 (MQ=255)
ttCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGgcgc > 1:4435779/1‑50 (MQ=255)
catcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGgcgc < 1:2299373/48‑1 (MQ=255)
catcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGctc > 1:1810631/1‑50 (MQ=255)
catcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGctc > 1:829254/1‑50 (MQ=255)
catcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGctc > 2:1988972/1‑50 (MQ=255)
catcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGct < 2:5168906/49‑1 (MQ=255)
catcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGct < 2:4249448/49‑1 (MQ=255)
catcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGct < 2:5405370/49‑1 (MQ=255)
catcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGctc > 2:2522438/1‑50 (MQ=255)
atcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGctct < 1:4496999/50‑1 (MQ=255)
atcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGctc < 2:4324336/49‑1 (MQ=255)
atcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGctc < 2:587465/49‑1 (MQ=255)
atcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGctct < 2:4071443/50‑1 (MQ=255)
atcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGctct < 1:64008/50‑1 (MQ=255)
atcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGctc < 1:1055190/49‑1 (MQ=255)
tcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCtt > 2:1384482/1‑50 (MQ=255)
tcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCtt > 2:2059234/1‑50 (MQ=255)
tcaAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCtt < 1:22770/50‑1 (MQ=255)
caAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCtt > 2:838210/1‑49 (MQ=255)
caAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCtt > 2:933672/1‑49 (MQ=255)
aaGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCttt < 2:2410586/49‑1 (MQ=255)
aaGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTa < 1:3855137/50‑1 (MQ=255)
aGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTaa > 1:4872862/1‑50 (MQ=255)
aGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTaa < 1:3848534/50‑1 (MQ=255)
aGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTaa < 1:2507354/50‑1 (MQ=255)
ggCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTaa > 2:2312915/1‑49 (MQ=255)
ggCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAat < 1:3565045/50‑1 (MQ=255)
gCTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAata < 2:3761070/50‑1 (MQ=255)
cTTTCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAatat < 1:1995588/50‑1 (MQ=255)
ttCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATaa > 1:5525478/1‑50 (MQ=255)
tCCAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATaa < 1:3714117/49‑1 (MQ=255)
ccAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATg > 1:1141449/1‑50 (MQ=255)
ccAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATg > 1:5210873/1‑50 (MQ=255)
ccAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAAt < 1:2843053/49‑1 (MQ=255)
cAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATg < 1:4234564/49‑1 (MQ=255)
cAGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGc > 1:5058941/1‑50 (MQ=255)
aGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGcc > 2:2752763/1‑50 (MQ=255)
aGCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGcc < 1:4389508/50‑1 (MQ=255)
gCTTTGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCg < 2:4093498/50‑1 (MQ=255)
ttGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGAt > 1:672636/1‑49 (MQ=255)
tGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATa < 2:1659914/49‑1 (MQ=255)
tGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATa < 2:1147931/49‑1 (MQ=255)
tGCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATa < 2:2435814/49‑1 (MQ=255)
tGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATa < 2:5157024/49‑1 (MQ=255)
gCCAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATaa < 1:4712407/49‑1 (MQ=255)
ccAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATaaa < 2:4768368/49‑1 (MQ=255)
ccAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATaaa < 1:1553565/49‑1 (MQ=255)
ccAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAag < 2:5563168/50‑1 (MQ=255)
cAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAag > 2:1755880/1‑49 (MQ=255)
cAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAag > 2:866534/1‑49 (MQ=255)
cAGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAag > 2:3733772/1‑49 (MQ=255)
cAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAag > 2:3547266/1‑49 (MQ=255)
aGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAag > 2:5694497/1‑48 (MQ=255)
aGCGCGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAag > 2:5380628/1‑48 (MQ=255)
gcgcGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAagcag < 2:3339463/50‑1 (MQ=255)
gcgcGGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAagcag < 1:3649151/50‑1 (MQ=255)
gcgcGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAagcag < 1:1405173/50‑1 (MQ=255)
cgcgGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAagcagc < 2:5588957/50‑1 (MQ=255)
gcgGAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGcc < 1:1319315/50‑1 (MQ=255)
ggAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTa > 1:3184863/1‑50 (MQ=255)
ggAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTa > 1:1762392/1‑50 (MQ=255)
ggAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTa > 1:1714171/1‑50 (MQ=255)
ggAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTa < 1:5263008/50‑1 (MQ=255)
ggAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTa < 1:5228315/50‑1 (MQ=255)
gAAGGGTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTAc > 1:5628001/1‑50 (MQ=255)
gAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTAc < 2:1609193/50‑1 (MQ=255)
cgggTGGTCTTTGGCGCTCTTTAATATAATGCAGATAAAGCAGCCTACgg > 2:2053574/2‑49 (MQ=255)
aGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGt > 2:1605714/1‑50 (MQ=255)
gggTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGt > 2:5291142/1‑49 (MQ=255)
gggTGGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTc < 2:1403541/50‑1 (MQ=255)
gggTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGt > 2:1695170/1‑49 (MQ=255)
gTAGTCTTTGGCGCTCTTTAATGTAATGCCGATAAAGCAGCCTACGTCAt < 1:3928155/50‑1 (MQ=255)
tAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCAt > 1:954697/1‑49 (MQ=255)
|
GCTTCAGTGACTTCATCAAGGCTTTCCAGCTTTGCCAGCGCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCAT > NC_000913/3926711‑3926807
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
