Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A24 F999 I0 R1	116	27.9	2350853	86.4%	2031136	49.6

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,034,771	C→T	A55V (GCT→GTT)	hemG →	protoporphyrinogen oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,034,771	0	C	T	96.9%	105.8 / ‑4.0	32	A55V (GCT→GTT)	hemG	protoporphyrinogen oxidase
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (17/14); total (18/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CATTGAAGAACCACAGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGCGTTCCAGGAATTTGTC  >  NC_000913/4034724‑4034817

cATTGAAGAACCACAGTGGGAAAACTATGACCGTGTGGTCATTGGTGttt                                              <  1:4857/50‑1 (MQ=255)

 aTTGAAGAACCACAGTGGGAAAACTATGACCGTGTGGTCATTGGTGTTTc                                             >  1:18934/1‑50 (MQ=255)

 aTTGAAGAACCACAGTGGGAAAACTATGACCGTGTGGTCATTGGTGTTTc                                             >  1:1829849/1‑50 (MQ=255)

         aCCACAGTGGGAAAACTATGACCGTGTGGTCATTGGTGTTTCTATTCGCt                                     <  1:1057029/50‑1 (MQ=255)

          ccACAGTGGGAAAACTATGACCGTGTGGTCATTGGTGTTTCTATTCGCTa                                    >  1:1992956/1‑50 (MQ=255)

          ccACAGTGGGAAAACTATGACCGTGTGGTCATTGGTGTTTCTATTCGCTa                                    >  1:145668/1‑50 (MQ=255)

            acaGTGGGAAAACTATGACCGTGTGGTCATTGGTGTTTCTATTCGCTATg                                  <  1:2158698/50‑1 (MQ=255)

            acaGTGGGAAAACTATGACCGTGTGGTCATTGGTGTTTCTATTCGCTATg                                  <  1:1499765/50‑1 (MQ=255)

             caGTGGGAAAACTATGACCGTGTGGTCATTGGTGTTTCTATTCGCTATgg                                 >  1:1587949/1‑50 (MQ=255)

               gTGGGAAAACTATGACCGTGTGGTCATTGGTGTTTCTATTCGCTATGGTc                               <  1:1286225/50‑1 (MQ=255)

                tGGGAAAACTATGACCGTGTGGTCATTGGTGTTTCTATTCGCTATGGTCa                              <  1:1917027/50‑1 (MQ=255)

                tGGGAAAACTATGACCGTGTGGTCATTGGTGTTTCTATTCGCTATGGTCa                              <  1:1082570/50‑1 (MQ=255)

                 gggAAAACTATGACCGTGTGGTCATTGGTGTTTCTATTCGCTATGGTCAc                             >  1:495154/1‑50 (MQ=255)

                 gggAAAACTATGACCGTGTGGTCATTGGTGTTTCTATTCGCTATGGTCAc                             >  1:2288892/1‑50 (MQ=255)

                  ggAAAACTATGACCGTGTGGTCATTGGTGTTTCTATTCGCTATGGTCAc                             <  1:694418/49‑1 (MQ=255)

                  ggAAAACTATGACCGTGTGGTCATTGGTGTTTCTATTCGCTATGGTCAc                             <  1:56132/49‑1 (MQ=255)

                   gAAAACTATGACCGTGTGGTCATTGGTGTTTCTATTCGCTATGGTCACTa                           <  1:1103753/50‑1 (MQ=255)

                    aaaaCTATGACCGTGTGGTCATTGGTGTTTCTATTCGCTATGGTCACTAc                          <  1:738047/50‑1 (MQ=255)

                    aaaaCTATGACCGTGTGGTCATTGGTGTTTCTATTCGCTATGGTCACTAc                          >  1:1268201/1‑50 (MQ=255)

                     aaaCTATGACCGTGTGGTCATTGGTGTTTCTATTCGCTATGGTCACTAcc                         <  1:2207077/50‑1 (MQ=255)

                     aaaCTATGACCGTGTGGTCATTGGTGTTTCTATTCGCTATGGTCACTAc                          >  1:1153719/1‑49 (MQ=255)

                      aaCTATGACCGTGTGGTCATTGGTGTTTCTATTCGCTATGGTCACTACCa                        <  1:377332/50‑1 (MQ=255)

                        cTATGACCGTGTGGTCATTGGTGTTTCTATTCGCTATGGTCACTACCAtt                      <  1:933869/50‑1 (MQ=255)

                               cGTGTGGTCATTGGTGTTTCTATTCGCTATGGTCACTACCATTCAGCGtt               >  1:1021842/1‑50 (MQ=255)

                                gtgtGGTCATTGGTGTTTCTATTCGCTATGGTCACTACCATTCAGCGTTc              >  1:242409/1‑50 (MQ=255)

                                 tgtgGTCATTGGTGTTTCTATTCGCTATGGTCACTACCATTCAGCGTTc              >  1:822394/1‑49 (MQ=255)

                                    ggTCATTGGTGTTTCTATTCGCTATGGTCACTACCATTCAGCGTTCCAg           >  1:921995/1‑49 (MQ=255)

                                     gTCATTGGTGTTTCTATTCGCTATGGTCACTACCATTCAGCGTTCCAgg          >  1:1552181/1‑49 (MQ=255)

                                     gTCATTGGTGTTTCTATTCGCTATGGTCACTACCATTCAGCGTTCCAgg          >  1:854847/1‑49 (MQ=255)

                                     gTCATTGGTGTTTCTATTCGCTATGGTCACTACCATTCAGCGTTCCAgg          <  1:888396/49‑1 (MQ=255)

                                        aTTGGTGTTTCTATTCGCTATGGTCACTACCATTCAGCGTTCCAGGAAtt      >  1:707567/1‑50 (MQ=255)

                                         ttGGTGTTTCTATTCGCTATGGTCACTACCATTCAGCGTTCCAGGAAttt     >  1:73667/1‑50 (MQ=255)

                                            gtgtTTCTATTCGCTATGGTCACTACCATTCAGCGTTCCAGGAATTTGTc  <  1:2255770/50‑1 (MQ=255)

                                            gtgtTTCTATTCGCTATGGTCACTACCATTCAGCGTTCCAGGAATTTGTc  <  1:1321625/50‑1 (MQ=255)

                                            gTGCTTCTATTCGCTATGGTCACTACCATTCAGCGTTCCAGGAATTTGt   >  1:1898856/1‑49 (MQ=255)

CATTGAAGAACCACAGTGGGAAAACTATGACCGTGTGGTCATTGGTGCTTCTATTCGCTATGGTCACTACCATTCAGCGTTCCAGGAATTTGTC  >  NC_000913/4034724‑4034817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

N/A