Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A26 F999 I0 R1 111 30.9 2867207 97.9% 2806995 48.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,926,696 C→T G103D (GGC→GAC)  asnC ← DNA‑binding transcriptional dual regulator AsnC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,926,6960CT100.0% 173.7 / NA 44G103D (GGC→GAC) asnCDNA‑binding transcriptional dual regulator AsnC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/14);  total (30/14)

AGCGTCGATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCT  >  NC_000913/3926650‑3926741
                                              |                                             
agCGTCGATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCg                                             >  1:1061585/1‑49 (MQ=255)
  cGTCGATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGt                                           >  1:2076530/1‑49 (MQ=255)
   gTCGATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGtt                                          <  1:824364/49‑1 (MQ=255)
   gTCGATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGtt                                          >  1:2209550/1‑49 (MQ=255)
   gTCGATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGtt                                          <  1:662817/49‑1 (MQ=255)
    tcgatcgaACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTtg                                         <  1:705409/49‑1 (MQ=255)
    tcgatcgaACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTtg                                         <  1:1658732/49‑1 (MQ=255)
       atcgaACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTtgtg                                       >  1:2477532/1‑48 (MQ=255)
        tcgaACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTgtgt                                      >  1:32877/1‑48 (MQ=255)
          gaACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTaa                                    >  1:472905/1‑48 (MQ=255)
           aaCGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTaa                                    >  1:2339377/1‑47 (MQ=255)
           aaCGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATa                                  <  1:2193312/49‑1 (MQ=255)
            aCGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAg                                 >  1:2572703/1‑49 (MQ=255)
             cGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAgg                                <  1:1717440/49‑1 (MQ=255)
              ggCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGc                               >  1:1167325/1‑49 (MQ=255)
                cacaTCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCtt                             >  1:2657705/1‑49 (MQ=255)
                cacaTCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCtt                             >  1:1016816/1‑49 (MQ=255)
                 acaTCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCtt                             >  1:2724289/1‑48 (MQ=255)
                 acaTCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCtt                             >  1:2368450/1‑48 (MQ=255)
                 acaTCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTc                            <  1:2014012/49‑1 (MQ=255)
                  caTCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCa                           <  1:1225005/49‑1 (MQ=255)
                      aCTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTg                        <  1:2341881/48‑1 (MQ=255)
                       cTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGAc                      >  1:1440556/1‑49 (MQ=255)
                        ttttATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACt                     >  1:798829/1‑49 (MQ=255)
                          ttATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACtt                    >  1:622546/1‑48 (MQ=255)
                           tataAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTtca                  >  1:2005813/1‑49 (MQ=255)
                           tataAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTtca                  >  1:204362/1‑49 (MQ=255)
                            ataAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTtca                  <  1:1724410/48‑1 (MQ=255)
                              aaaGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTtcatc                >  1:2233081/1‑48 (MQ=255)
                               aaGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTtcatc                >  1:2390101/1‑47 (MQ=255)
                                aGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAg             <  1:495207/49‑1 (MQ=255)
                                 gATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAgg            >  1:2514088/1‑49 (MQ=255)
                                  aTGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGc           >  1:782144/1‑49 (MQ=255)
                                   tGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCt          <  1:1519725/49‑1 (MQ=255)
                                    gCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCtt         >  1:2274691/1‑49 (MQ=255)
                                    gCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCtt         >  1:1754231/1‑49 (MQ=255)
                                    gCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCtt         >  1:1361694/1‑49 (MQ=255)
                                       gTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTc       >  1:1773604/1‑48 (MQ=255)
                                        tAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCa     <  1:1229358/49‑1 (MQ=255)
                                         aGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAg    >  1:1913112/1‑49 (MQ=255)
                                          gTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGc   <  1:2734647/49‑1 (MQ=255)
                                          gTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGc   >  1:987973/1‑49 (MQ=255)
                                           tGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCt  >  1:781562/1‑49 (MQ=255)
                                           tGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCt  >  1:1654818/1‑49 (MQ=255)
                                              |                                             
AGCGTCGATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCT  >  NC_000913/3926650‑3926741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A