Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I52 R1	239	220.4	11079197	93.2%	10325811	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,769,648	(C)_6→7	intergenic (+162/‑55)	yfjQ → / → yfjR	CP4‑57 prophage; uncharacterized protein/CP4‑57 prophage; putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,769,642	1	.	C	95.6%	612.3 / 21.1	183	intergenic (+156/‑61)	yfjQ/yfjR	CP4‑57 prophage; uncharacterized protein/CP4‑57 prophage; putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand): ref base . (5/3); new base C (89/86); total (94/89)
Fisher's exact test for biased strand distribution p-value = 7.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.02e-01

Only 100 of 212 total aligned reads displayed.

CTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑CC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGG  >  NC_000913/2769544‑2769736

cTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑c                                                                                                   <  1:5401794/100‑1 (MQ=255)

    tgtgCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑cc‑ccc                                                                                              <  2:778635/100‑1 (MQ=255)

     gtgCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑cc‑ccc‑‑‑c                                                                                          <  1:555061/100‑1 (MQ=255)

     gtgCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCATGTCCG‑cc‑ccc‑‑‑c                                                                                          >  1:136532/1‑100 (MQ=255)

      tgCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑cc‑ccc‑‑‑cc                                                                                         <  1:4699746/100‑2 (MQ=255)

      tgCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAAGAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑cc‑ccc‑‑‑cc                                                                                         >  1:1551618/1‑99 (MQ=255)

       gCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑cc‑ccc‑‑‑cct                                                                                        >  2:1638213/1‑98 (MQ=255)

       gCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑cc‑ccc‑‑‑cct                                                                                        >  1:2015230/1‑98 (MQ=255)

        ccTCAATCCCCCGGTTATAGCCTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTCCGTTGTCCATATCCTGTCCG‑cc‑ccc‑‑‑ccta                                                                                       >  1:684998/1‑97 (MQ=25)

         cTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑cc‑ccc‑‑‑cctct                                                                                      <  2:1807733/100‑5 (MQ=255)

          tCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑cc‑ccc‑‑‑cctctt                                                                                     <  1:3590431/100‑6 (MQ=255)

          tCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑cc‑ccc‑‑‑cctctt                                                                                     <  2:782290/100‑6 (MQ=255)

          tCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑CC‑CCC‑‑‑CTCttt                                                                                     <  2:4402322/100‑1 (MQ=255)

           cAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCttt                                                                                     <  1:4277976/100‑1 (MQ=255)

           cAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCttt                                                                                     <  2:1024902/100‑1 (MQ=255)

            aaTCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTa                                                                                    <  2:273439/100‑1 (MQ=255)

            aaTCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTa                                                                                    <  2:3258421/100‑1 (MQ=255)

             aTCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTaa                                                                                   <  2:354356/100‑1 (MQ=255)

             aTCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTaa                                                                                   <  1:1338669/100‑1 (MQ=255)

              tCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTaaa                                                                                  <  1:1500436/100‑1 (MQ=255)

               cccccGGTTTTAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAg                                                                                 <  2:4766408/100‑1 (MQ=255)

                ccccGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGt                                                                                <  2:1850819/100‑1 (MQ=255)

                ccccGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGt                                                                                <  2:3639695/100‑1 (MQ=255)

                 cccGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTa                                                                               >  1:2597833/1‑100 (MQ=255)

                  ccGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTaa                                                                              <  2:5094422/100‑1 (MQ=255)

                   cGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAAt                                                                             <  2:2153741/100‑1 (MQ=255)

                    ggTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATc                                                                            <  1:2779692/100‑1 (MQ=255)

                    ggTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATc                                                                            <  1:1861383/100‑1 (MQ=255)

                     gTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATca                                                                           >  2:4145499/1‑100 (MQ=255)

                         taGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCAcatc                                                                       >  2:5430801/1‑100 (MQ=255)

                          aGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCAcatca                                                                      >  2:3974652/1‑100 (MQ=255)

                          aGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCAcatca                                                                      >  1:970003/1‑100 (MQ=255)

                             ttttAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCAttt                                                                   >  2:2385589/1‑100 (MQ=255)

                             ttttAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCAttt                                                                   >  2:3883776/1‑100 (MQ=255)

                              tttAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCAtttt                                                                  >  1:5026320/1‑100 (MQ=255)

                               ttAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTtc                                                                 <  2:3381786/100‑1 (MQ=255)

                               ttAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTtc                                                                 <  2:1315528/100‑1 (MQ=255)

                               ttAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTtc                                                                 <  2:4526389/100‑1 (MQ=255)

                               ttAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTCTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTtc                                                                 <  1:4980503/100‑1 (MQ=255)

                                 aaCCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTtcag                                                               <  1:462775/100‑1 (MQ=255)

                                   cccccGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTtcagtc                                                             >  1:3200778/1‑100 (MQ=255)

                                   cccccGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTtcagtc                                                             >  1:4131306/1‑100 (MQ=255)

                                     cccGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTtcagtcag                                                           >  1:3469479/1‑100 (MQ=255)

                                        gTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTa                                                        >  2:3527953/1‑100 (MQ=255)

                                        gTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTa                                                        <  2:718756/100‑1 (MQ=255)

                                          tACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAAc                                                      <  1:3818624/100‑1 (MQ=255)

                                          tACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAAc                                                      <  1:754451/100‑1 (MQ=255)

                                          tACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAAc                                                      <  2:3779433/100‑1 (MQ=255)

                                            naTCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCGGCCC‑CCC‑‑‑CTCTGTAAAGTAATGACATCATATTCAGTCAGTTAACtt                                                    >  1:4455262/2‑100 (MQ=14)

                                            cATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACtt                                                    >  1:4061265/1‑100 (MQ=255)

                                            cATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACtt                                                    <  1:2333960/100‑1 (MQ=255)

                                              tCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTc                                                  >  2:4625932/1‑100 (MQ=255)

                                              tCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTc                                                  >  1:5230952/1‑100 (MQ=255)

                                               cTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTcc                                                 <  2:3707292/100‑1 (MQ=255)

                                               cTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTcc                                                 >  1:1593945/1‑100 (MQ=255)

                                               cTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTcc                                                 <  1:5004787/100‑1 (MQ=255)

                                                   cTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGga                                             >  2:844475/1‑100 (MQ=255)

                                                    ttttGCAGAAATAAAACATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGgag                                            <  2:939989/100‑1 (MQ=17)

                                                    ttttGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGgag                                            <  1:2426256/100‑1 (MQ=255)

                                                    ttttGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGgag                                            <  2:4024921/100‑1 (MQ=255)

                                                      ttGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGaa                                          <  1:3917363/100‑1 (MQ=255)

                                                       tGCACAAATAAAAAATAGTTTCTTCCTTCTACATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAATTAATCACATCATTTTCAGTCAGTTAACTTTCCTTGAGAAt                                         <  1:4826817/100‑1 (MQ=17)

                                                        gCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAAtc                                        >  1:402955/1‑100 (MQ=255)

                                                         cAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAAtct                                       <  2:4900141/100‑1 (MQ=255)

                                                          aGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGCAtctc                                      >  1:2234509/1‑100 (MQ=255)

                                                            aaaTAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAAtctctc                                    <  1:1657954/100‑1 (MQ=255)

                                                            aaaTAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAAtctctc                                    <  2:3318100/100‑1 (MQ=255)

                                                             aaTAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCa                                   <  1:2155940/100‑1 (MQ=255)

                                                             aaTAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCa                                   >  2:1960419/1‑100 (MQ=255)

                                                              aTAAAAAATAGTTTCTGCGTTGTCCATACCCTGTGCGCCC‑CCC‑‑‑CTCTTTAAAGAAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCAt                                  >  1:5171059/1‑100 (MQ=21)

                                                              aTAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑CCACCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCAt                                  >  2:4226150/1‑100 (MQ=255)

                                                                 aaaaaTAGTTTCTGCGTTATCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGac                               >  1:70271/1‑100 (MQ=255)

                                                                  aaaaTAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCGTTTGCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGaca                              <  2:5159506/100‑1 (MQ=18)

                                                                    aaTAGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGAcaca                            <  2:4093962/100‑1 (MQ=255)

                                                                       aGTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGc                         <  2:1442675/100‑1 (MQ=255)

                                                                        ntttCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCa                        >  1:271769/2‑100 (MQ=255)

                                                                        gTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCa                        >  1:2960399/1‑100 (MQ=255)

                                                                        gTTTCTGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCa                        <  1:4520581/100‑1 (MQ=255)

                                                                            ccacGTTGTCCATACCCGGTCCGCCC‑CCC‑‑‑CTCTTAAAATTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAAc                    <  2:2523403/97‑1 (MQ=17)

                                                                             tGCGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAAcg                   <  1:3382082/100‑1 (MQ=255)

                                                                             tGCGTTGTCCATACCCTGTCCG‑CC‑CCCCCTCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGaa                     >  2:4854395/1‑100 (MQ=18)

                                                                               cGTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAAcgcc                 >  2:3155110/1‑100 (MQ=255)

                                                                                gTTGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAAcgccg                >  2:851524/1‑100 (MQ=255)

                                                                                  tGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACCCAGGCAGAACgccgcc              >  2:2747963/1‑100 (MQ=255)

                                                                                  tGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACgccgcc              >  1:3705234/1‑100 (MQ=255)

                                                                                  tGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACgccgcc              <  1:2097062/100‑1 (MQ=255)

                                                                                  tGTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACgccgcc              <  2:3071686/100‑1 (MQ=255)

                                                                                   gTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCa             >  2:2988808/1‑100 (MQ=255)

                                                                                   gTCCATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCa             >  2:555973/1‑100 (MQ=255)

                                                                                    tCCATACCCTGTCCG‑CC‑CCCA‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCAt            >  1:4346874/1‑100 (MQ=255)

                                                                                     ccttgcccTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTGAGTTTACTTTCCTGGAGAAGCTCTCATGACACAGGCAGAACGCGGCCATg           >  2:5176639/6‑100 (MQ=17)

                                                                                     ccATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATg           >  2:2845233/1‑100 (MQ=255)

                                                                                      cATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGa          <  1:2310023/100‑1 (MQ=255)

                                                                                      cATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGa          >  1:1031872/1‑100 (MQ=255)

                                                                                      cATACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGa          >  2:4062060/1‑100 (MQ=255)

                                                                                       aTACCCTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGAc         >  2:5033787/1‑100 (MQ=255)

                                                                                          cccTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCgg      >  2:2125575/1‑100 (MQ=255)

                                                                                            cTGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCggct    <  1:3068128/100‑1 (MQ=255)

                                                                                             tGTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCggctg   <  2:2595116/100‑1 (MQ=255)

                                                                                              gTCCGCCC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCggctgg  >  1:3310036/1‑100 (MQ=255)

CTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCG‑CC‑CCC‑‑‑CTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGG  >  NC_000913/2769544‑2769736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A