Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,044,628 A→C K323Q (AAG→CAG)  bglA → 6‑phospho‑beta‑glucosidase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,044,6280AC97.9% 834.1 / 4.4 240K323Q (AAG→CAG) bglA6‑phospho‑beta‑glucosidase A
Reads supporting (aligned to +/- strand):  ref base A (2/3);  new base C (126/108);  total (128/112)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01

Only 100 of 249 total aligned reads displayed.

CGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATC  >  NC_000913/3044531‑3044724
                                                                                                 |                                                                                                
 ggATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAgg                                                                                               <  2:1202960/100‑1 (MQ=255)
    tttAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCg                                                                                            >  1:3762125/1‑100 (MQ=255)
    tttAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCg                                                                                            >  1:3898897/1‑100 (MQ=255)
     ttAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGa                                                                                           >  2:4792238/1‑100 (MQ=255)
     ttAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGa                                                                                           >  2:2688713/1‑100 (MQ=255)
       aaCATCAAAATGGAAGACGGAGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAg                                                                                         <  1:2798302/100‑1 (MQ=255)
         cATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAggc                                                                                       <  1:5409640/100‑1 (MQ=255)
          ctCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGGACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAggcg                                                                                      <  1:4715456/99‑1 (MQ=255)
          aTCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAggcg                                                                                      >  1:594420/1‑100 (MQ=255)
          aTCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAggcg                                                                                      >  2:1440066/1‑100 (MQ=255)
           tCAAAATGGAAGACAGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGCTTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAggcgg                                                                                     >  1:4889375/1‑100 (MQ=255)
           ctaaaaTGTAAGCCAGCGATCTTGATGTGCTGGGTGAAGGCTCCTGCGATTATCTTGGTTCCAGCTCTTACATTACCAATGCAGTGCAGGCCGAAggcgg                                                                                     <  2:632822/98‑1 (MQ=255)
            cAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAggcggc                                                                                    >  2:868807/1‑100 (MQ=255)
            cAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAggcggc                                                                                    <  1:4543904/100‑1 (MQ=255)
             aaaaTGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAggcggag                                                                                   >  1:4304569/1‑98 (MQ=255)
              aaaTGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCAc                                                                                  <  2:1653549/100‑1 (MQ=255)
                 tGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCgg                                                                               <  2:1610986/100‑1 (MQ=255)
                   gAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGgcg                                                                             >  2:3423041/1‑100 (MQ=255)
                     aGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGgcgat                                                                           <  2:4636464/100‑1 (MQ=255)
                      gACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGgcgatg                                                                          >  2:4193623/1‑100 (MQ=255)
                      gACGGCGATCTGGATGTGCCGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACAGGACCATTGCAGTGCAGGCCGAAGGCGGCACCGgcgatg                                                                          <  1:4954625/100‑1 (MQ=255)
                       cttccGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGgcgatgc                                                                         <  1:1451157/96‑1 (MQ=255)
                       aCGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGgcgatgc                                                                         >  2:4888304/1‑100 (MQ=255)
                       aCGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGgcgatgc                                                                         <  1:847126/100‑1 (MQ=255)
                        cGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGgcgatgcg                                                                        >  1:1039681/1‑100 (MQ=255)
                        cGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGgcgatgcg                                                                        >  2:2183512/1‑100 (MQ=255)
                        cGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGgcgatgcg                                                                        >  1:1184115/1‑100 (MQ=255)
                        cGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGgcgatgcg                                                                        <  1:288975/100‑1 (MQ=255)
                          gCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGgcgatgcgat                                                                      <  1:3440232/100‑1 (MQ=255)
                          gCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGgcgatgcgat                                                                      <  2:2908489/100‑1 (MQ=255)
                           cGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGAtc                                                                     >  2:1852393/1‑100 (MQ=255)
                           cGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGAtc                                                                     >  1:3668706/1‑100 (MQ=255)
                             aTCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGAtctc                                                                   <  1:928062/100‑1 (MQ=255)
                                tGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTgg                                                                >  1:4085337/1‑100 (MQ=255)
                                tGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTgg                                                                <  2:274033/100‑1 (MQ=255)
                                  gATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGtt                                                              >  1:746305/1‑100 (MQ=255)
                                   aTGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGttt                                                             <  1:1873245/100‑1 (MQ=255)
                                    tgtgCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGtttt                                                            <  1:3609548/100‑1 (MQ=255)
                                    tctgctgcGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACGTGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTACGGtttt                                                            >  2:5105271/3‑100 (MQ=255)
                                      tgctgcGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGa                                                          >  1:2092340/1‑100 (MQ=255)
                                       gctgcGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGaa                                                         >  1:1061381/1‑100 (MQ=255)
                                       gctgcGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGaa                                                         <  1:1030380/100‑1 (MQ=255)
                                       gctgcGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGaa                                                         >  1:4500639/1‑100 (MQ=255)
                                       gctgcGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGaa                                                         <  1:2703549/100‑1 (MQ=255)
                                       gctgcGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGaa                                                         >  1:4126031/1‑100 (MQ=255)
                                        ctgcGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAg                                                        >  2:3786308/1‑100 (MQ=255)
                                        ctgcGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAg                                                        >  2:2267827/1‑100 (MQ=255)
                                         tgcGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAgg                                                       >  2:1835123/1‑100 (MQ=255)
                                         tgcGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAgg                                                       >  2:920559/1‑100 (MQ=255)
                                           cGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCa                                                     <  1:3512311/100‑1 (MQ=255)
                                            gTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAg                                                    >  1:242062/1‑100 (MQ=255)
                                             tGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGc                                                   >  1:2995680/1‑100 (MQ=255)
                                                 ggCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTAc                                               <  2:239684/100‑1 (MQ=255)
                                                 ggCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTAc                                               <  1:1634262/100‑1 (MQ=255)
                                                  gCAGCTGCTATTAACTTGGTTTCAGCGACGACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTAcc                                              <  1:2212209/100‑1 (MQ=255)
                                                   cACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCa                                             <  1:5139377/100‑1 (MQ=255)
                                                        gCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAAccc                                        <  2:4001329/100‑1 (MQ=255)
                                                        gCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAAccc                                        <  1:418343/100‑1 (MQ=255)
                                                        gCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAAccc                                        >  2:4593930/1‑100 (MQ=255)
                                                         cGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCg                                       <  2:2462508/100‑1 (MQ=255)
                                                          gATTATCTTGGTTGCAGCTATTACATGACCATTGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGt                                      <  2:4316730/100‑1 (MQ=255)
                                                           aTTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTa                                     <  1:5518747/100‑1 (MQ=255)
                                                           aTTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTa                                     <  2:3195931/100‑1 (MQ=255)
                                                           aTTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTa                                     <  1:791506/100‑1 (MQ=255)
                                                            ttATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTAt                                    <  2:1795182/100‑1 (MQ=255)
                                                            ttATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTAt                                    <  1:3711096/100‑1 (MQ=255)
                                                             tctCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATg                                   <  2:123927/98‑1 (MQ=255)
                                                             tATCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATg                                   <  1:5364511/100‑1 (MQ=255)
                                                              aTCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGt                                  <  2:1121351/100‑1 (MQ=255)
                                                              aTCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGt                                  >  1:3317575/1‑100 (MQ=255)
                                                               tCTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGTCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGtt                                 >  2:1966134/1‑100 (MQ=255)
                                                                cTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATgaga                                >  1:125201/1‑97 (MQ=255)
                                                                cTTGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCATATGTTa                                <  2:4745562/100‑1 (MQ=255)
                                                                  tGGTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTaaa                              >  1:1948536/1‑100 (MQ=255)
                                                                   ggTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCCCCGGCGCTGCGATCTCTGGTTTTGAAGCCAGCGTACCAACCCCGTATGTTAAAg                             <  2:5136268/100‑1 (MQ=255)
                                                                   ggTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAg                             <  2:1472667/100‑1 (MQ=255)
                                                                   ggTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAg                             >  1:243987/1‑100 (MQ=255)
                                                                    gTTTCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGc                            >  2:2372699/1‑100 (MQ=255)
                                                                       tCAGCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATc                         <  1:3306781/100‑1 (MQ=255)
                                                                         ngCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTg                       >  1:4410596/2‑100 (MQ=255)
                                                                          gCTATTACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGa                      >  1:2255185/1‑100 (MQ=255)
                                                                               tACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTggg                 <  1:5323759/100‑1 (MQ=255)
                                                                               tACATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTggg                 <  1:2773581/100‑1 (MQ=255)
                                                                                aCATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTgggg                >  2:284732/1‑100 (MQ=255)
                                                                                 cATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGc               >  2:377393/1‑100 (MQ=255)
                                                                                 cATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGc               >  2:1568603/1‑100 (MQ=255)
                                                                                 cATGACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGc               >  2:169660/1‑100 (MQ=255)
                                                                                    gACCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTgg            >  1:1408818/1‑100 (MQ=255)
                                                                                     aCCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGc           <  1:3957791/100‑1 (MQ=255)
                                                                                     aCCAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGc           <  2:5475649/100‑1 (MQ=255)
                                                                                      ccAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCa          >  1:3066612/1‑100 (MQ=255)
                                                                                      ccAATGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCa          >  2:5247310/1‑100 (MQ=255)
                                                                                       cAATGCAGTGCAGGCCGAAGGCGGCACGGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAg         <  2:5258826/100‑1 (MQ=255)
                                                                                        aaTGCAGTGCAGGCCGAAGGCGGCACCGGCGATGCGCTCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGa        >  2:3390501/1‑100 (MQ=255)
                                                                                          tgcagtgcagGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGAtt      <  1:4950659/100‑1 (MQ=255)
                                                                                          tgcagtgcagGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGAtt      >  2:3708151/1‑100 (MQ=255)
                                                                                          tgcagtgcagGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTGGGAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGAtt      <  2:3299428/100‑1 (MQ=255)
                                                                                             aGTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGAt   <  1:3815205/100‑1 (MQ=255)
                                                                                              gTGCAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATc  >  2:2452425/1‑100 (MQ=255)
                                                                                              gTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATc  <  2:4928343/100‑1 (MQ=255)
                                                                                                 |                                                                                                
CGGATTTAACATCAAAATGGAAGACGGCGATCTGGATGTGCTGCGTGAAGGCACCTGCGATTATCTTGGTTTCAGCTATTACATGACCAATGCAGTGAAGGCCGAAGGCGGCACCGGCGATGCGATCTCTGGTTTTGAAGGCAGCGTACCAAACCCGTATGTTAAAGCATCTGACTGGGGCTGGCAGATTGATC  >  NC_000913/3044531‑3044724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A