Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,174,035 T→G E454D (GAA→GAC)  parE ← DNA topoisomerase IV, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,174,0350TG97.1% 827.5 / 0.5 241E454D (GAA→GAC) parEDNA topoisomerase IV, subunit B
Reads supporting (aligned to +/- strand):  ref base T (3/4);  new base G (103/130);  total (107/134)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 252 total aligned reads displayed.

TACAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCC  >  NC_000913/3173938‑3174130
                                                                                                 |                                                                                               
  cAGGTTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCAcgtcgt                                                                                             >  2:5055034/1‑100 (MQ=255)
  cAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCAcgtcgt                                                                                             >  2:3803052/1‑100 (MQ=255)
  cAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAAGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCAcgtcgt                                                                                             >  1:4735070/1‑100 (MQ=255)
  cAGATTTTGCCATAACGAAGCTGGCGCAGATTGCCGCCTTCAGGACCGATACCAATCGCTACCGCAATACCCTGCACATCCCGCTAACCGATCAcgtcgt                                                                                             >  2:3537610/1‑100 (MQ=255)
    gATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGtcgtcg                                                                                           >  1:3048130/1‑100 (MQ=255)
    gATTTTGCCATAACGAAGCTGGCTCAGATAGTCGCTGTAAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGACGCCAGCAcgtcgtgg                                                                                           >  2:1770495/1‑98 (MQ=255)
       tttGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGaa                                                                                        <  2:4065937/100‑1 (MQ=255)
        ttGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAag                                                                                       <  1:5241785/100‑1 (MQ=255)
          gCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGACATCTCGTGCACTTCCTTCGAAGCCAGCACGTCGTCGGAagtc                                                                                     <  2:1278193/100‑3 (MQ=255)
          gCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAagag                                                                                     >  1:3778505/1‑100 (MQ=255)
           ccATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAgaga                                                                                    >  1:4574008/1‑100 (MQ=255)
           ccATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAgaga                                                                                    >  1:4461823/1‑100 (MQ=255)
            cATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGAc                                                                                   <  1:3538454/100‑1 (MQ=255)
            cATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGAc                                                                                   >  2:420670/1‑100 (MQ=255)
            cATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGAc                                                                                   >  1:5071501/1‑100 (MQ=255)
              tAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACtt                                                                                 <  2:2354278/100‑1 (MQ=255)
               aaCGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTc                                                                                >  2:1156670/1‑100 (MQ=255)
                aCGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTcc                                                                               >  2:2008618/1‑100 (MQ=255)
                 cGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTccc                                                                              <  2:3213907/100‑1 (MQ=255)
                   aaGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAg                                                                            <  1:2135090/100‑1 (MQ=255)
                    aGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAgg                                                                           <  1:2534418/100‑1 (MQ=255)
                      cTGGGTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGgtg                                                                         >  2:4655792/1‑100 (MQ=255)
                        ggCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTCCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGtt                                                                       <  2:5475108/100‑1 (MQ=255)
                         gCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGATGAGACTTCCCACGTGTTa                                                                      <  1:4649489/100‑1 (MQ=255)
                         gCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTa                                                                      <  2:5337797/100‑1 (MQ=255)
                          cTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTaa                                                                     >  1:4591891/1‑100 (MQ=255)
                           tCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAg                                                                    <  2:3040665/100‑1 (MQ=255)
                            cAGATCGTCGCTGTCAGGATCGATACCGATCGCTCCCGAAATATCGTGCACTTGCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAgg                                                                   >  1:5165582/1‑100 (MQ=255)
                              gATCGTCGCTGTCAGGATCGCTACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAGGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGAt                                                                 >  1:4343626/1‑100 (MQ=255)
                              gATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAgggt                                                                 >  2:683938/1‑98 (MQ=255)
                              gATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGAt                                                                 >  2:3149683/1‑100 (MQ=255)
                              gATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGAt                                                                 >  2:3391085/1‑100 (MQ=255)
                              gATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGAt                                                                 >  1:1469342/1‑100 (MQ=255)
                               aTCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATc                                                                <  2:1123657/100‑1 (MQ=255)
                               aTCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATc                                                                <  2:3729687/100‑1 (MQ=255)
                               aTCGTCGCTGACAGGAACGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACCTCCCAGGTGTTAAGGATc                                                                >  1:4874423/1‑100 (MQ=255)
                                tcgtcgCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCt                                                               <  1:4193287/100‑1 (MQ=255)
                                 cgtcgCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCtt                                                              >  2:4333253/1‑100 (MQ=255)
                                  gtcgCTGTCAGGATCGATACCGATCGCTACCGGAATATAGTGCACTGCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTACCAGGTGTTAAGGaagatc                                                             >  1:4954909/1‑95 (MQ=255)
                                  gtcgCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTa                                                             >  1:2620285/1‑100 (MQ=255)
                                    cgCTGTCAGGATCGATCCCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTAcc                                                           <  2:5494045/100‑1 (MQ=255)
                                      cTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCtt                                                         >  2:3561302/1‑100 (MQ=255)
                                      cTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCtt                                                         <  2:4418992/100‑1 (MQ=255)
                                       tGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCttt                                                        >  1:3657713/1‑100 (MQ=255)
                                        gTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTc                                                       >  2:52925/1‑100 (MQ=255)
                                          cAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAg                                                     >  2:2696941/1‑100 (MQ=255)
                                          cAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAg                                                     <  2:1448995/100‑1 (MQ=255)
                                          cAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAg                                                     <  2:1450409/100‑1 (MQ=255)
                                          cAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAg                                                     >  2:1803917/1‑100 (MQ=255)
                                          cAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAg                                                     <  2:3268224/100‑1 (MQ=255)
                                           atcttCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGt                                                    <  1:10184/96‑1 (MQ=255)
                                            ggATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCCCGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTg                                                   >  1:2403611/1‑100 (MQ=255)
                                            ggATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTg                                                   >  2:237714/1‑100 (MQ=255)
                                                cGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCAt                                               <  2:1726724/100‑1 (MQ=255)
                                                 gATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATg                                              >  2:5034727/1‑100 (MQ=255)
                                                 gATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATg                                              >  2:4627624/1‑100 (MQ=255)
                                                     ccGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCg                                          <  1:2100148/100‑1 (MQ=255)
                                                      cGATCGCTACCGAAATAGCGTGCACTTCGTGCGAAACCAGCACGTTGTCGGAAGAGACTTCCCAGGTGTTAAGGAGCTTACCTTTCAGTGTCATGATCGc                                         >  2:1296827/1‑100 (MQ=255)
                                                       gATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGcc                                        >  1:674762/1‑100 (MQ=255)
                                                       gATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGcc                                        <  1:2696553/100‑1 (MQ=255)
                                                       gATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGcc                                        <  1:4331348/100‑1 (MQ=255)
                                                       gATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGcc                                        <  1:138525/100‑1 (MQ=255)
                                                        aTCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCt                                       <  2:601922/100‑1 (MQ=255)
                                                         tCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTg                                      <  2:1002443/100‑1 (MQ=255)
                                                         tCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTg                                      <  2:2198970/100‑1 (MQ=255)
                                                         tCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTg                                      <  2:26063/100‑1 (MQ=255)
                                                          cGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGa                                     <  1:4685762/100‑1 (MQ=255)
                                                            cTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGata                                   <  1:177185/100‑1 (MQ=255)
                                                            cTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGACCGCCTGata                                   >  1:225077/1‑100 (MQ=255)
                                                              aCCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAGAGACGTCCCAGTTGTGAAGGAGCGTACCGTTCAGTGTCATGATCGGCTGatatg                                 >  2:1625432/1‑99 (MQ=255)
                                                              aCCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATAtt                                 <  1:3850218/100‑1 (MQ=255)
                                                              aCCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATAtt                                 >  2:3457792/1‑100 (MQ=255)
                                                               ccGAAATATCGTGCAGTTCCTGCGAAGCCAGAACGTCGTCGGAAGAGACTTCCCAGGGGCTAAGGAACTTATCTTTCAGTGGCATGACCGCCTGATATTc                                >  2:4172739/1‑100 (MQ=255)
                                                               ccGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTc                                >  1:4100180/1‑100 (MQ=255)
                                                               ccGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTc                                >  1:2480394/1‑100 (MQ=255)
                                                                cGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTcg                               >  1:5548349/1‑100 (MQ=255)
                                                                 gAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTcgc                              >  1:1722148/1‑100 (MQ=255)
                                                                  taaTATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTcgcg                             <  2:82711/99‑1 (MQ=255)
                                                                  aaaTATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTcgcg                             <  1:2015866/100‑1 (MQ=255)
                                                                     tatCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATc                          <  2:3466911/100‑1 (MQ=255)
                                                                     tatCGCGTACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGCTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATc                          <  2:5114408/100‑1 (MQ=255)
                                                                       tCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATcgc                        <  2:4896436/100‑1 (MQ=255)
                                                                         gTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATcgcgc                      >  1:639379/1‑100 (MQ=255)
                                                                            cACTTCCTGAGAAGCCATCTCTTCGTTGGCAGAGTCTTCCCAGGTGTTAAGATTCTTTCCTTTCAGTGGCATGATCGCCTGATATTCGTGATCGCGCGcc                   <  2:666585/100‑1 (MQ=255)
                                                                              cTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTg                 >  2:3835116/1‑100 (MQ=255)
                                                                                 ccTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCtt              <  1:2777089/100‑1 (MQ=255)
                                                                                  cTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTg             >  1:2005549/1‑100 (MQ=255)
                                                                                  cTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTg             >  1:2680571/1‑100 (MQ=255)
                                                                                  cTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTg             <  1:3091082/100‑1 (MQ=255)
                                                                                  cTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTg             >  1:1604463/1‑100 (MQ=255)
                                                                                  cTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTg             <  1:3422871/100‑1 (MQ=255)
                                                                                   tGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTgg            <  2:3654999/100‑1 (MQ=255)
                                                                                   tGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTAgg            >  2:735803/1‑100 (MQ=255)
                                                                                    gCGAAGCCAGCACGTCGTCGGAATAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGc           <  1:5563165/100‑1 (MQ=255)
                                                                                    gCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGc           <  1:1208480/100‑1 (MQ=255)
                                                                                    gCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGc           >  1:5454427/1‑100 (MQ=255)
                                                                                      gAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAg         >  2:2912708/1‑100 (MQ=255)
                                                                                       aaGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGa        <  1:3163650/100‑1 (MQ=255)
                                                                                           cAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCg    <  1:3003116/100‑1 (MQ=255)
                                                                                             gCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGcc  <  1:1877512/100‑1 (MQ=255)
                                                                                                 |                                                                                               
TACAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCC  >  NC_000913/3173938‑3174130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A