Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I53 R1 229 158.0 8090706 91.1% 7370633 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,174,035 T→G E454D (GAA→GAC)  parE ← DNA topoisomerase IV, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,174,0350TG98.9% 645.0 / ‑2.3 182E454D (GAA→GAC) parEDNA topoisomerase IV, subunit B
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base G (91/89);  total (93/89)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01

Only 100 of 188 total aligned reads displayed.

TACAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCT  >  NC_000913/3173938‑3174131
                                                                                                 |                                                                                                
tACAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGtc                                                                                                <  1:1343946/100‑1 (MQ=255)
  cAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCAcgtcgt                                                                                              <  1:1841162/100‑1 (MQ=255)
   aGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACgtcgtc                                                                                             >  1:2878151/1‑100 (MQ=255)
    gATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGtcgtcg                                                                                            >  1:3153636/1‑100 (MQ=255)
           ccATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAgaga                                                                                     <  1:1028140/100‑1 (MQ=255)
           ccATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAgaga                                                                                     <  1:1948396/100‑1 (MQ=255)
            cATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGAc                                                                                    >  2:2534949/1‑100 (MQ=255)
            cATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGAc                                                                                    >  1:2941127/1‑100 (MQ=255)
             aTAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACt                                                                                   >  2:2685986/1‑100 (MQ=255)
             aTAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACt                                                                                   >  2:2881750/1‑100 (MQ=255)
             aTAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCCTCGGAAGAGACt                                                                                   >  2:2146694/1‑100 (MQ=255)
              tAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACtt                                                                                  >  1:2930351/1‑100 (MQ=255)
               aaCGAAGCTGGCTCAGATCGTCTCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTc                                                                                 >  2:1307350/1‑100 (MQ=255)
               aaCGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTc                                                                                 >  2:3632068/1‑100 (MQ=255)
                aCGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTcc                                                                                >  2:3947465/1‑100 (MQ=255)
                 cGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGCAGCCAGCACGTCGTCGGAAGAGACTTacc                                                                               >  2:2269549/1‑100 (MQ=255)
                   aaGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAg                                                                             <  1:1438914/100‑1 (MQ=255)
                   aaGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAg                                                                             <  2:2082418/100‑1 (MQ=255)
                    aGCTGGCTCAGAGCGTCGCTGTCAGGATCGATACCGATCGCTACCTAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAgg                                                                            <  2:2692832/100‑1 (MQ=255)
                     gCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGgt                                                                           >  2:2124717/1‑100 (MQ=255)
                        ggCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGtt                                                                        >  1:207941/1‑100 (MQ=255)
                           tCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAg                                                                     <  2:1080527/100‑1 (MQ=255)
                             aGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGTACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGa                                                                   <  2:3975161/100‑1 (MQ=255)
                             aGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGa                                                                   <  2:3054511/100‑1 (MQ=255)
                              gATCGTAGCTGTCAGGATCGATACCGATCGCTACCGAAATATAGTGCACTTCCTGCGAAGCCAGCAAGTCGTAGAAAGAGACTTCCCAGGTGTTAAGGAt                                                                  >  2:1191737/1‑100 (MQ=255)
                                  gtcgCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTa                                                              <  2:1644962/100‑1 (MQ=255)
                                   tcgCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGAAAAACCAAGAGTTAAGAATCTTAc                                                             <  2:807698/100‑1 (MQ=255)
                                    cgCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTAcc                                                            <  1:1984390/100‑1 (MQ=255)
                                    cgCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTAcc                                                            <  2:1390260/100‑1 (MQ=255)
                                     gCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCt                                                           <  2:2937723/100‑1 (MQ=255)
                                      cTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCtt                                                          >  2:298291/1‑100 (MQ=255)
                                       tGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCttt                                                         >  1:1596556/1‑100 (MQ=255)
                                        gTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTc                                                        <  1:3123137/100‑1 (MQ=255)
                                         tCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCa                                                       <  2:1274671/100‑1 (MQ=255)
                                           aGGATGGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGt                                                     <  2:1685926/100‑1 (MQ=255)
                                           aGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCATGTGTTAAGGATCTTACCTTTCAGt                                                     <  2:648900/100‑1 (MQ=255)
                                            ggATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTg                                                    >  2:2675576/1‑100 (MQ=255)
                                            ggATCGATACCGAGCGCTACCGAAATAACGTGCACTTCCTGCGAAGCCAGCACGTCGTCAGAAGAGACTTCCCAGGTGTTAAGGATCTAACCTTTCAGTg                                                    >  1:3599506/1‑100 (MQ=255)
                                             gATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTgg                                                   <  1:3267687/100‑1 (MQ=255)
                                             gATCGATACCGATCGCTACCGAAATATCGGGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTgg                                                   <  2:3960865/100‑1 (MQ=255)
                                              aTCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGc                                                  <  1:2727415/100‑1 (MQ=255)
                                              aTCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGc                                                  <  1:2157947/100‑1 (MQ=255)
                                               tCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCa                                                 <  2:3085477/100‑1 (MQ=255)
                                               tCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCa                                                 <  2:1560639/100‑1 (MQ=255)
                                                 gttACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATg                                               <  1:2454533/98‑1 (MQ=255)
                                                 gATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATg                                               >  1:1828644/1‑100 (MQ=255)
                                                 gATACCGATCGCTACCGAAATATCGTGCACTTCCTGCAAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGGGTTAAGGATCTTACCTTTCAGTGGCATg                                               >  2:900288/1‑100 (MQ=255)
                                                  aTACCGATCGCTACCGAAATATCGTGCACTTCATGCAAAGCCAGCACGTCGTCGTAAGCGACTTACCAGGTGTTAAGGCTCTTACCTTCCAGTGGCATGa                                              >  2:1997165/1‑100 (MQ=255)
                                                        aTCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCt                                        >  1:2103839/1‑100 (MQ=255)
                                                         tCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTg                                       <  1:3444156/100‑1 (MQ=255)
                                                           gCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGat                                     >  2:2695415/1‑100 (MQ=255)
                                                            cTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGata                                    <  1:2661240/100‑1 (MQ=255)
                                                            cTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGata                                    >  1:590958/1‑100 (MQ=255)
                                                            cTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGata                                    >  2:3611757/1‑100 (MQ=255)
                                                             tACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTCCCTTTCCGTGGCATGATCGCCTGatct                                   >  1:3321413/1‑98 (MQ=255)
                                                             tACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGatat                                   <  1:441563/100‑1 (MQ=255)
                                                              aCCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATAtt                                  >  1:857207/1‑100 (MQ=255)
                                                              aCCGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATAtt                                  >  1:1997154/1‑100 (MQ=255)
                                                               ccGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTc                                 >  2:2717812/1‑100 (MQ=255)
                                                               ccGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTc                                 >  1:149329/1‑100 (MQ=255)
                                                               ccGAAATATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTc                                 >  1:605802/1‑100 (MQ=255)
                                                                  aaaTATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTcgcg                              <  2:933854/100‑1 (MQ=255)
                                                                  aaaTATCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTcgcg                              <  2:2310889/100‑1 (MQ=255)
                                                                    atatCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGAt                            >  2:3377481/1‑100 (MQ=255)
                                                                      atCGTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATcg                          <  1:1639287/100‑1 (MQ=255)
                                                                         gTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATcgcgc                       <  1:2919047/100‑1 (MQ=255)
                                                                         gTGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATcgcgc                       >  1:1045226/1‑100 (MQ=255)
                                                                          tGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATcgcgcg                      <  1:3690430/100‑1 (MQ=255)
                                                                          tGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATcgcgcg                      >  1:760400/1‑100 (MQ=255)
                                                                          tGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATcgcgcg                      <  1:2671715/100‑1 (MQ=255)
                                                                          tGCACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGCGTTAAGGATCTCACCTTTCAGTGGCATGATCCCCTGATATTCGCCATcgcgcg                      >  2:1602293/1‑100 (MQ=255)
                                                                            cACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGcc                    <  1:3966404/100‑1 (MQ=255)
                                                                            cACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGcc                    >  1:112730/1‑100 (MQ=255)
                                                                            cACTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGcc                    <  2:2834829/100‑1 (MQ=255)
                                                                             aCTTCCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCt                   >  1:1094761/1‑100 (MQ=255)
                                                                             aCTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCt                   >  2:3684522/1‑100 (MQ=255)
                                                                              cTTCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTg                  >  2:832406/1‑100 (MQ=255)
                                                                               ttCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGc                 <  1:102233/100‑1 (MQ=255)
                                                                                tCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCt                <  2:1908264/100‑1 (MQ=255)
                                                                                tCCTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCt                >  1:905826/1‑100 (MQ=255)
                                                                                  cTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTg              >  2:709542/1‑100 (MQ=255)
                                                                                  cTGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCCCCTGCTTg              <  2:3832896/100‑1 (MQ=255)
                                                                                   tGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTgg             <  2:3194992/100‑1 (MQ=255)
                                                                                   tGCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTgg             >  2:686221/1‑100 (MQ=255)
                                                                                    gCGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGc            <  2:3256820/100‑1 (MQ=255)
                                                                                     cGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCa           >  2:1606570/1‑100 (MQ=255)
                                                                                     cGAAGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCa           >  1:3699841/1‑100 (MQ=255)
                                                                                     cGAAGCCAGCACGTCGTCGGAAGAGACCTCCCAGGTGTTAAGGATCTTACCTTTCAGTGCCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCa           <  1:3535504/100‑1 (MQ=255)
                                                                                     cGAAGCCAGCACGCCGGCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCa           <  2:1942633/100‑1 (MQ=255)
                                                                                       aaGCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGa         <  2:1718643/100‑1 (MQ=255)
                                                                                         gCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCCGTCGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATc       >  2:2172297/1‑100 (MQ=255)
                                                                                         gCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATc       >  1:2332104/1‑100 (MQ=255)
                                                                                         gCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATc       >  1:3000302/1‑100 (MQ=255)
                                                                                         gCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATc       >  1:3303931/1‑100 (MQ=255)
                                                                                         gCCAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATc       >  1:528483/1‑100 (MQ=255)
                                                                                          ccAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATcc      <  1:2801291/100‑1 (MQ=255)
                                                                                          ccAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATcc      <  2:1725259/100‑1 (MQ=255)
                                                                                           cAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCg     >  2:2238428/1‑100 (MQ=255)
                                                                                           cAGCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCg     <  2:1822495/100‑1 (MQ=255)
                                                                                             gCACGTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGcc   >  2:1162561/1‑100 (MQ=255)
                                                                                                 |                                                                                                
TACAGATTTTGCCATAACGAAGCTGGCTCAGATCGTCGCTGTCAGGATCGATACCGATCGCTACCGAAATATCGTGCACTTCCTGCGAAGCCAGCACTTCGTCGGAAGAGACTTCCCAGGTGTTAAGGATCTTACCTTTCAGTGGCATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCT  >  NC_000913/3173938‑3174131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A