Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I52 R1	239	220.4	11079197	93.2%	10325811	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,752,058	A→C	I23L (ATC→CTC)	yiaU →	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,752,058	0	A	C	99.6%	816.6 / ‑5.9	234	I23L (ATC→CTC)	yiaU	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (83/150); total (84/150)
Fisher's exact test for biased strand distribution p-value = 3.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 236 total aligned reads displayed.

ATATAAAATTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACC  >  NC_000913/3751961‑3752154

 tataAATTTATATATAATTGGGGAGTTTTAATGATATAATTACATCTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAGTCTCa                                                                                                <  2:2605695/100‑1 (MQ=255)

 tataAAATTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCa                                                                                                <  1:2797386/100‑1 (MQ=255)

 tataAAATTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCa                                                                                                <  1:1733827/100‑1 (MQ=255)

   taAAATTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGc                                                                                              <  1:2992935/100‑1 (MQ=255)

   taAAATTATATATAATTAGGCTGTTGCGTTGAAAAAATTACAAATTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGc                                                                                              <  2:3516422/100‑1 (MQ=255)

    aaaaTTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGcc                                                                                             <  1:541297/100‑1 (MQ=255)

    aaaaTTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGcc                                                                                             <  1:4242669/100‑1 (MQ=255)

    aaaaTTATATATAATTGGGCTGTTGCGATGAAAAAATTACAACTTAAGTAAAAAAAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGcc                                                                                             <  2:3138523/100‑1 (MQ=255)

     aaaTTATATAGAATTGGGCTGTTGCTATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCa                                                                                            <  2:4813826/100‑1 (MQ=255)

      caTTACATATAAGTAGGCTGTTGCGATGAAAAAAATACAAATTAATTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCACTGAAAATCTCAGCCAt                                                                                           <  2:5124835/99‑1 (MQ=255)

      aaTTATATATAATTGGGATGTTGAGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCAt                                                                                           <  2:3368810/100‑1 (MQ=255)

       aTTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATg                                                                                          >  1:2192791/1‑100 (MQ=255)

            atataATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCcgcg                                                                                     <  1:874108/100‑1 (MQ=255)

            atataATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCcgcg                                                                                     >  1:2609832/1‑100 (MQ=255)

            atataATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCcgcg                                                                                     >  1:274609/1‑100 (MQ=255)

             tataATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGa                                                                                    >  2:1744495/1‑100 (MQ=255)

              ataATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGAc                                                                                   <  1:929946/100‑1 (MQ=255)

               aaaTTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACt                                                                                  <  2:1352655/99‑1 (MQ=255)

                aaTTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTg                                                                                 <  1:3275153/100‑1 (MQ=255)

                aaTTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTg                                                                                 >  1:1071840/1‑100 (MQ=255)

                 aTTGGGCTGTTGCAATGACAAAAATACAACTTAAATACAGAAAATTAAAAATTATCTCGGTAATCGCTGCCAGTAAAAATCTAAGACATGACGCGACTGt                                                                                <  2:204446/100‑1 (MQ=255)

                  ttGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTa                                                                               <  1:767912/100‑1 (MQ=255)

                   tGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTAc                                                                              >  2:4098440/1‑100 (MQ=255)

                   tGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTAc                                                                              <  2:2725485/100‑1 (MQ=255)

                   tGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACCGTAc                                                                              <  1:3874212/100‑1 (MQ=255)

                     ggCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACtt                                                                            >  1:1119933/1‑100 (MQ=255)

                      gCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTg                                                                           >  2:2558114/1‑100 (MQ=255)

                       cTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTgg                                                                          <  1:1299130/100‑1 (MQ=255)

                         gTTCCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCa                                                                        >  2:5054576/1‑100 (MQ=255)

                          ttGCGATGACAAAAATACAACTTACGTACCGGGAGATAAATATTATCTCGGTAACCGCCGCCAGTGAAAACCTCAGCCATGCCGCGACTGTACTTGGCAt                                                                       <  2:3485219/100‑1 (MQ=255)

                           tGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATc                                                                      <  1:3423061/100‑1 (MQ=255)

                           tGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATc                                                                      <  2:4310397/100‑1 (MQ=255)

                            gCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCg                                                                     >  1:381364/1‑100 (MQ=255)

                              gATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCACTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGca                                                                   >  2:5402925/1‑100 (MQ=255)

                                tGAGAAAATTACAACTTAAGTACCGGGAGTAAAAAATTATCTCGGCAATCTCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCACCCcaca                                                                 <  2:5181688/100‑1 (MQ=255)

                                   cAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGc                                                              >  1:1106232/1‑100 (MQ=255)

                                   cAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGc                                                              >  2:2274917/1‑100 (MQ=255)

                                    aaaaTTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGcc                                                             <  1:2487705/100‑1 (MQ=255)

                                        ttACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACg                                                         <  2:2151786/100‑1 (MQ=255)

                                         tACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGt                                                        >  1:2847632/1‑100 (MQ=255)

                                           cAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCa                                                      <  1:1886549/100‑1 (MQ=255)

                                           cAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAATGAAAATCTCAGCCAGGCCGCGACTGTACTTAGCATCGCACAGGCCAACGTCa                                                      >  1:5185805/1‑100 (MQ=255)

                                             aCTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGc                                                    >  2:3688378/1‑100 (MQ=255)

                                             aCTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGc                                                    >  1:4387736/1‑100 (MQ=255)

                                             aCTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGc                                                    >  2:3005964/1‑100 (MQ=255)

                                             aCTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGc                                                    <  1:2920243/100‑1 (MQ=255)

                                              cTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCTATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCa                                                   >  1:3086432/1‑100 (MQ=255)

                                              cTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCa                                                   >  2:1214471/1‑100 (MQ=255)

                                                tAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCaa‑a                                                >  1:4778940/1‑100 (MQ=255)

                                                  aGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ata                                              <  1:4525180/100‑1 (MQ=255)

                                                     aCCGGGATTTAAAAAGTATCACGGTAATCGCTGCTAGTGAAAATCTTAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCt                                           <  2:2317775/100‑1 (MQ=255)

                                                      ccGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCtt                                          >  1:5400458/1‑100 (MQ=255)

                                                      ccGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCtt                                          <  1:5125215/100‑1 (MQ=255)

                                                      ccGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCtt                                          >  1:1218862/1‑100 (MQ=255)

                                                      ccGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCtt                                          <  1:4697372/100‑1 (MQ=255)

                                                       cGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTg                                         <  2:5827/100‑1 (MQ=255)

                                                       cGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTg                                         <  1:461611/100‑1 (MQ=255)

                                                       cGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTg                                         <  2:4004567/100‑1 (MQ=255)

                                                       cGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACTTGGCAACGCACAGGCCAACGTAAGCAA‑ATAACtta                                         >  2:3269078/1‑99 (MQ=255)

                                                        gggAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGc                                        <  2:5293647/100‑1 (MQ=255)

                                                        gggAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGc                                        <  1:2006030/100‑1 (MQ=255)

                                                        gggAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGc                                        <  1:3918000/100‑1 (MQ=255)

                                                            gTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAGATAT‑TTGCTGac                                    <  1:1552292/100‑2 (MQ=255)

                                                            gTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGAt                                    >  2:1552596/1‑100 (MQ=255)

                                                            gTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGAt                                    >  1:1655934/1‑100 (MQ=255)

                                                             ttAAAAATTATCTCGGTAATCGCCGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGAtt                                   <  1:4685973/100‑1 (MQ=255)

                                                              tAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAGATAT‑TTGCTGacgt                                  <  1:2036709/100‑4 (MQ=255)

                                                              tAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGAttt                                  >  1:4791956/1‑100 (MQ=255)

                                                              tAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGAttt                                  >  2:1436862/1‑100 (MQ=255)

                                                               aaaaaTTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGAtttt                                 <  2:785149/100‑1 (MQ=255)

                                                                aaaaTTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGATTTTg                                <  2:2820537/100‑1 (MQ=255)

                                                                aaaaTTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGATTTTg                                >  1:3752881/1‑100 (MQ=255)

                                                                aaaaTTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTCCTGATTTTg                                <  1:5148464/100‑1 (MQ=255)

                                                                 aaaTTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGATTTTGa                               >  2:4697541/1‑100 (MQ=255)

                                                                   aTTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGATTTTGAAt                             <  1:3196200/100‑1 (MQ=255)

                                                                   aTTATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGATTTTGAAt                             <  2:4505649/100‑1 (MQ=255)

                                                                    ttATCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGATTTTGAATc                            <  1:1404336/100‑1 (MQ=255)

                                                                    ttATCTCGGTAATCGCTGCCAGTAAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGATTTTGAATc                            >  2:4599886/1‑100 (MQ=255)

                                                                      aTCTCGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGATTTTGAATCaa                          >  1:4468527/1‑100 (MQ=255)

                                                                       tctcGGTAATCGCTGCCAGTGAAAATCTGAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGATTTTGAATCaaa                         <  1:5055311/100‑1 (MQ=255)

                                                                       tctcGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGATTTTGAATCaaa                         <  2:662966/100‑1 (MQ=255)

                                                                       tctcGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGATTTTGAATCaaa                         <  2:1144463/100‑1 (MQ=255)

                                                                        ctcGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGATTTTGAATCaaaa                        <  1:4181505/100‑1 (MQ=255)

                                                                         tcGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGATTTTGAATCaaacg                       >  2:5077887/1‑98 (MQ=255)

                                                                         tcGGTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGATTTTGAATCAAAAg                       >  2:1983367/1‑100 (MQ=255)

                                                                           ggTAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGATTTTGAATCAAAAGTg                     <  2:2489324/100‑1 (MQ=255)

                                                                             tAATCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGATTTTGAATCAAAAGTggg                   <  2:508802/100‑1 (MQ=255)

                                                                              aaTCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGATTTTGAATCAAAAGTGGGt                  <  1:3532057/100‑1 (MQ=255)

                                                                               aTCGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGATTTTGAATCAAAAGTGGGtt                 <  2:1003713/100‑1 (MQ=255)

                                                                                 cGCTGCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGATTTTGAATCAAAAGTGGGTTTa               >  1:4921011/1‑100 (MQ=255)

                                                                                  gCTGCAAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGATTTTGAATCAAAAGTGGGTTTaa              >  2:3891746/1‑100 (MQ=255)

                                                                                     gCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAg           <  1:1014835/100‑1 (MQ=255)

                                                                                     gCCAGTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAg           <  1:1678438/100‑1 (MQ=255)

                                                                                         gTGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCtt       >  2:2985287/1‑100 (MQ=255)

                                                                                          tGAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCttt      <  1:685065/100‑1 (MQ=255)

                                                                                           gAAAATCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTg     <  2:3654895/100‑1 (MQ=255)

                                                                                              aaTCTCAGCCATGCCGCGACTGTACTTGGCATCGCATAGGCCAACGTAAGCAA‑ATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGAcc  <  2:3871574/100‑1 (MQ=255)

                                                                                              aaTCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCTA‑ATATCTTGCTGATTTTGAAGCAAAAGTGGGTTTAAAAGTCTTTGAcc  <  2:5473579/100‑1 (MQ=255)

                                                                                              aaTCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGAcc  <  2:2794578/100‑1 (MQ=255)

                                                                                              aaTCTCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAAAGTCAGAAA‑ATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGAcc  <  2:4163778/100‑1 (MQ=255)

ATATAAAATTATATATAATTGGGCTGTTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAA‑ATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACC  >  NC_000913/3751961‑3752154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A